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    Methods of and apparatus for washing high-density microplates
    1.
    发明授权
    Methods of and apparatus for washing high-density microplates 失效
    洗涤高密度微孔板的方法和设备

    公开(公告)号：US07516749B2

    公开(公告)日：2009-04-14

    申请号：US10408108

    申请日：2003-04-08

    申请人： Stephen Felder ,  Richard Kris ,  Brian McGraw

    发明人： Stephen Felder ,  Richard Kris ,  Brian McGraw

    IPC分类号： B08B6/00

    CPC分类号： B08B9/093 ,  B01L3/5085 ,  B01L99/00 ,  B01L2300/0829 ,  B08B3/02 ,  B08B9/00 ,  B08B9/0826 ,  Y10T436/11 ,  Y10T436/114998

    摘要： Methods of and apparatus for washing an array of sites in high-density microplates or similar assay plates wherein the microplates or assay plates are washed in an inverted or nearly inverted position, rather than in an upright position. Preferably, the wash liquid is dispensed upwardly in the form of a sheet from a nozzle mounted on a spray bar as the spray bar moves relative to the microplate or assay plate. After washing, the microplate or assay plate is dried with a stream of gas such as air, also preferably blown upwardly in the form of a sheet.

    摘要翻译： 在高密度微孔板或类似测定板中洗涤位点阵列的方法和装置，其中微孔板或测定板以颠倒或接近倒置的位置而不是直立位置洗涤。 优选地，当喷雾杆相对于微板或测定板移动时，洗涤液体以安装在喷雾杆上的喷嘴从片材的形式向上分配。 洗涤后，用气流例如空气干燥微板或测定板，最好以片状向上吹。

    High throughput assay system
    5.
    发明授权
    High throughput assay system 有权

    公开(公告)号：US06759198B2

    公开(公告)日：2004-07-06

    申请号：US09888413

    申请日：2001-06-26

    申请人： Richard M. Kris ,  Stephen Felder

    发明人： Richard M. Kris ,  Stephen Felder

    IPC分类号： C12Q168

    摘要： The present invention relates to compositions, apparatus and methods useful for concurrently performing multiple, high throughput, biological or chemical assays, using repeated arrays of probes. A combination of the invention comprises a surface, which comprises a plurality of test regions, at least two of which, and in a preferred embodiment, at least twenty of which, are substantially identical, wherein each of the test regions comprises an array of generic anchor molecules. The anchors are associated with bifunctional linker molecules, each containing a portion which is specific for at least one of the anchors and a portion which is a probe specific for a target of interest. The resulting array of probes is used to analyze the presence or test the activity of one or more target molecules which specifically interact with the probes. In a preferred embodiment, a sample to be tested is subjected to a nuclease protection procedure before it is contacted with a combination of the invention.

    HIGH THROUGHPUT ASSAY SYSTEM
    6.
    发明申请
    HIGH THROUGHPUT ASSAY SYSTEM 审中-公开
    高速测试系统

    公开(公告)号：US20100105572A1

    公开(公告)日：2010-04-29

    申请号：US12325699

    申请日：2008-12-01

    申请人： Richard M. KRIS ,  Stephen Felder

    发明人： Richard M. KRIS ,  Stephen Felder

    IPC分类号： C40B30/06

    CPC分类号： C12Q1/6816 ,  B01J2219/00315 ,  B01J2219/00527 ,  B01J2219/00547 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00702 ,  B01J2219/00722 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  C40B70/00 ,  C12Q2565/519 ,  C12Q2521/307 ,  C12Q2565/513 ,  C12Q2537/125

    摘要： The present invention relates to compositions, apparatus and methods useful for concurrently performing multiple, high throughput, biological or chemical assays, using repeated arrays of probes. A combination of the invention comprises a surface, which comprises a plurality of test regions, at least two of which, and in a preferred embodiment, at least twenty of which, are substantially identical, wherein each of the test regions comprises an array of generic anchor molecules. The anchors are associated with bifunctional linker molecules, each containing a portion which is specific for at least one of the anchors and a portion which is a probe specific for a target of interest. The resulting array of probes is used to analyze the presence or test the activity of one or more target molecules which specifically interact with the probes. In a preferred embodiment, a sample to be tested is subjected to a nuclease protection procedure before it is contacted with a combination of the invention.

    摘要翻译： 本发明涉及使用重复的探针阵列同时进行多次，高通量，生物或化学测定的组合物，装置和方法。 本发明的组合包括表面，其包括多个测试区域，其中至少两个，在优选实施例中，其至少二十个基本相同，其中每个测试区域包括通用的阵列 锚分子。 锚固体与双功能连接子分子缔合，每个含有对于至少一个锚的特异性的部分和作为感兴趣靶标的探针的部分。 所得到的探针阵列用于分析存在或测试与探针特异性相互作用的一种或多种靶分子的活性。 在优选的实施方案中，在与本发明的组合物接触之前，待测试样品经受核酸酶保护程序。

    High throughput assay system
    8.
    发明授权
    High throughput assay system 失效

    公开(公告)号：US06232066B1

    公开(公告)日：2001-05-15

    申请号：US09109076

    申请日：1998-07-02

    申请人： Stephen Felder ,  Richard M. Kris

    发明人： Stephen Felder ,  Richard M. Kris

    IPC分类号： C12Q168

    CPC分类号： C40B30/04 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  C40B40/06

    摘要： The present invention relates to compositions, apparatus and methods useful for concurrently performing multiple, high throughput, biological or chemical assays, using repeated arrays of probes. A combination of the invention comprises a surface, which comprises a plurality of test regions, at least two of which, and in a preferred embodiment, at least twenty of which, are substantially identical, wherein each of the test regions comprises an array of generic anchor molecules. The anchors are associated with bifunctional linker molecules, each containing a portion which is specific for at least one of the anchors and a portion which is a probe specific for a target of interest. The resulting array of probes is used to analyze the presence or test the activity of one or more target molecules which specifically interact with the probes. In one embodiment of the invention, the test regions (which can be wells) are further subdivided into smaller subregions (indentations, or dimples).

    High throughput assay system for monitoring ESTs
    9.
    发明授权
    High throughput assay system for monitoring ESTs 失效
    用于监测ESTs的高通量测定系统

    公开(公告)号：US06458533B1

    公开(公告)日：2002-10-01

    申请号：US09218089

    申请日：1998-12-22

    申请人： Stephen Felder ,  Bruce Seligmann ,  Richard M. Kris

    发明人： Stephen Felder ,  Bruce Seligmann ,  Richard M. Kris

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6827 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  C12Q1/6837 ,  C12Q1/6874 ,  C40B30/04 ,  C40B40/06 ,  C12Q2563/131 ,  C12Q2525/197 ,  C12Q2565/627 ,  C12Q2565/514 ,  C12Q2521/301

    摘要： The present invention relates to compositions, apparatus and methods useful for concurrently performing multiple, high throughput, biological or chemical assays, using repeated arrays of probes. A combination of the invention comprises a surface, which comprises a plurality of test regions, at least two of which, and in a preferred embodiment, at least twenty of which, are substantially identical, wherein each of the test regions comprises an array of generic anchor molecules. The anchors are associated with bifunctional linker molecules, each containing a portion which is specific for at least one of the anchors and a portion which is a probe specific for a target of interest. The resulting array of probes is used to analyze the presence or test the activity of one or more target molecules which specifically interact with the probes. In one embodiment of the invention, the test regions (which can be wells) are further subdivided into smaller subregions (indentations, or dimples). In one embodiment of the invention, ESTs are mapped. In another embodiment, the presence of a target nucleic acid is detected by protecting the target against nuclease digestion with a polynucleotide fragment, and analyzing the protected polynucleotide by mass spectrometry.

    摘要翻译： 本发明涉及使用重复的探针阵列同时进行多次，高通量，生物或化学测定的组合物，装置和方法。 本发明的组合包括表面，其包括多个测试区域，其中至少两个，在优选实施例中，其至少二十个基本相同，其中每个测试区域包括通用的阵列 锚分子。 锚固体与双功能连接子分子缔合，每个含有对于至少一个锚的特异性的部分和作为感兴趣靶标的探针的部分。 所得到的探针阵列用于分析存在或测试与探针特异性相互作用的一种或多种靶分子的活性。 在本发明的一个实施例中，测试区域（其可以是孔）进一步细分为更小的子区域（凹陷或凹坑）。在本发明的一个实施例中，映射了EST。 在另一个实施方案中，通过用多核苷酸片段保护靶标免于核酸酶消化来检测靶核酸的存在，并通过质谱分析受保护的多核苷酸。