-
公开(公告)号：US07932077B2
公开(公告)日：2011-04-26
申请号：US11265761
申请日：2005-11-02
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于25％二十碳五烯酸（EPA，ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株包括表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使EPA能够合成和高度积累。 要求生产宿主细胞，以及在所述宿主细胞内生产EPA的方法。
-
公开(公告)号：US20090233346A1
公开(公告)日：2009-09-17
申请号：US12469467
申请日：2009-05-20
CPC分类号： C12N15/815 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of Yarrowia for synthesis of ARA and EPA.
-
公开(公告)号：US07550286B2
公开(公告)日：2009-06-23
申请号：US11264737
申请日：2005-11-01
申请人： Howard Glenn Damude , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。 该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。 要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。
-
公开(公告)号：US08518674B2
公开(公告)日：2013-08-27
申请号：US12893469
申请日：2010-09-29
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于25％二十碳五烯酸（EPA，ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株包括表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使EPA能够合成和高度积累。 要求生产宿主细胞，以及在所述宿主细胞内生产EPA的方法。
-
公开(公告)号：US07588931B2
公开(公告)日：2009-09-15
申请号：US11264784
申请日：2005-11-01
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 10% arachidonic acid (ARA, an ω-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ARA. Production host cells are claimed, as are methods for producing ARA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于10％花生四烯酸（ARA，ω-6多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株包含表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使ARA能够合成和高度积累。 要求生产宿主细胞，以及在所述宿主细胞内产生ARA的方法。
-
公开(公告)号：US07553628B2
公开(公告)日：2009-06-30
申请号：US11714377
申请日：2007-03-05
CPC分类号： C12N15/815 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of Yarrowia for synthesis of ARA and EPA.
摘要翻译： 本发明涉及在含油酵母中生产ω-3和/或ω-6脂肪酸的方法。 因此，能够催化亚油酸（LA）转化成γ-亚麻酸（GLA）的去饱和酶和延伸酶; α-亚油酸（ALA）至十八碳四烯酸（STA）; GLA至二氢-γ-亚油酸（DGLA）; STA至二十碳四烯酸（ETA）; DGLA对花生四烯酸（ARA）; ETA至二十碳五烯酸（EPA）; DGLA到ETA; EPA至二十二碳五烯酸（DPA）; 并且ARA至EPA已被引入耶氏酵母的基因组合成ARA和EPA。
-
公开(公告)号：US20090311380A1
公开(公告)日：2009-12-17
申请号：US12466072
申请日：2009-05-14
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。 该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。 要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。
-
公开(公告)号：US07238482B2
公开(公告)日：2007-07-03
申请号：US10840579
申请日：2004-05-06
CPC分类号： C12N15/815 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of Yarrowia for synthesis of ARA and EPA.
-
公开(公告)号：US08399242B2
公开(公告)日：2013-03-19
申请号：US12469467
申请日：2009-05-20
CPC分类号： C12N15/815 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of Yarrowia for synthesis of ARA and EPA.
-
公开(公告)号：US20110086919A1
公开(公告)日：2011-04-14
申请号：US12893469
申请日：2010-09-29
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , Dana M. Walters Pollak , James John Ragghianti , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
IPC分类号： A61K31/202 , C12N1/15 , C12P7/64 , C07C57/03 , A61P9/00 , A61P3/10 , A61P19/02 , A61P1/00 , A61P17/02 , A61P19/10
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于25％二十碳五烯酸（EPA，ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株包括表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使EPA能够合成和高度积累。 要求生产宿主细胞，以及在所述宿主细胞内生产EPA的方法。
-
-
-
-
-
-
-
-
-
搜索结果
77 条记录 (耗时 0.046 秒)
