公开(公告)号：US08962262B2

公开(公告)日：2015-02-24

申请号：US11747830

申请日：2007-05-11

申请人： Stephen Lovell ,  Lydia Blank ,  Virginia Crews ,  Nancy Hasse ,  Peter Lu ,  Johannes Schweizer ,  John Mantlo

发明人： Stephen Lovell ,  Lydia Blank ,  Virginia Crews ,  Nancy Hasse ,  Peter Lu ,  Johannes Schweizer ,  John Mantlo

IPC分类号： C07K16/18 ,  C07K14/47 ,  A61K45/06 ,  G01N33/569

CPC分类号： C07K14/005 ,  C07K1/145 ,  C07K14/82 ,  C12N7/00 ,  C12N2710/20051 ,  G01N33/56983 ,  G01N2333/025

摘要： Methods for producing a protein extract from cells, such as cells containing viral proteins, are provided. In general terms, the methods involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent, neutralizing the pH of the intermediate composition to produce the protein extract. Kits and compositions for practicing the subject methods are also provided.

摘要翻译： 提供了从细胞如含有病毒蛋白的细胞产生蛋白质提取物的方法。 一般来说，所述方法包括：将细胞的pH增加至至少约pH 10.0的pH以产生中间体组合物，然后在非离子型洗涤剂的存在下将中间体组合物的pH中和至 产生蛋白质提取物。 还提供了用于实践主题方法的工具包和作品。

公开(公告)号：US09207240B2

公开(公告)日：2015-12-08

申请号：US11985547

申请日：2007-11-14

申请人： Adrien P. Malick ,  Virginia M. Crews ,  Julie L. Rosales ,  Carrie S. Ferguson ,  Jeff H. Bruton ,  Robert J. Beadenkopf ,  John Mantlo

发明人： Adrien P. Malick ,  Virginia M. Crews ,  Julie L. Rosales ,  Carrie S. Ferguson ,  Jeff H. Bruton ,  Robert J. Beadenkopf ,  John Mantlo

IPC分类号： C12N15/52 ,  C07K1/14 ,  G01N33/569

CPC分类号： G01N33/54393 ,  C07K1/145 ,  C12N15/52 ,  C12N2710/20022 ,  G01N1/4044 ,  G01N33/56983 ,  G01N2333/025 ,  G01N2333/16 ,  G01N2469/10

摘要： Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.