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    Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
    1.
    发明授权
    Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites 有权
    选择锌指蛋白靶向位点和设计锌指蛋白结合预选位点的方法

    公开(公告)号：US06785613B2

    公开(公告)日：2004-08-31

    申请号：US10113424

    申请日：2002-03-28

    申请人： Stephen P. Eisenberg ,  Casey C. Case ,  George N. Cox, III ,  Andrew Jamieson ,  Edward J. Rebar

    发明人： Stephen P. Eisenberg ,  Casey C. Case ,  George N. Cox, III ,  Andrew Jamieson ,  Edward J. Rebar

    IPC分类号： G06F1900

    CPC分类号： C12N15/1089 ,  C07K14/47 ,  C07K14/4702 ,  C12Q1/6811 ,  G01N33/5308 ,  G01N33/6875 ,  G06F19/18

    摘要： The invention provides criteria and methods for selecting optimum subsequence(s) from a target gene for targeting by a zinc finger protein. Some of the methods of target site selection seek to identify one or more target segments having a DNA motif containing one or more so-called D-able subsites having the sequence 5′NNGK3′. Other methods of the invention are directed to selection of target segments within target genes using a correspondence regime between different triplets of three bases and the three possible positions of a triplet within a nine-base site. In another aspect, the invention provides methods of designing zinc finger proteins that bind to a preselected target site. These methods can be used following the preselection of target sites according to the procedures and criteria described above. The methods of design use a database containing information about previously characterized zinc finger proteins.

    摘要翻译： 本发明提供了从靶基因中选择锌指蛋白靶向的最佳亚序列的标准和方法。 靶位点选择的一些方法寻求鉴定具有含有一个或多个具有序列5'NNGK3'的一个或多个所谓的D能子集的DNA基序的一个或多个靶区段。 本发明的其它方法涉及使用三个碱基的不同三联体之间的对应方式和九个碱基位点内三联体的三个可能位置来选择靶基因内的靶区段。 另一方面，本发明提供了设计结合预选靶位点的锌指蛋白的方法。 这些方法可以根据上述程序和标准在预选目标位点之后使用。 设计方法使用包含以前特征的锌指蛋白的信息的数据库。

    Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
    2.
    发明授权
    Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites 有权
    选择锌指蛋白靶向位点和设计锌指蛋白结合预选位点的方法

    公开(公告)号：US06453242B1

    公开(公告)日：2002-09-17

    申请号：US09229007

    申请日：1999-01-12

    申请人： Stephen P. Eisenberg ,  Casey C. Case ,  George N. Cox, III ,  Andrew Jamieson ,  Edward J. Rebar

    发明人： Stephen P. Eisenberg ,  Casey C. Case ,  George N. Cox, III ,  Andrew Jamieson ,  Edward J. Rebar

    IPC分类号： G06F1900

    CPC分类号： C12N15/1089 ,  C07K14/47 ,  C07K14/4702 ,  C12Q1/6811 ,  G01N33/5308 ,  G01N33/6875 ,  G06F19/18

    摘要： The invention provides criteria and methods for selecting optimum subsequence(s) from a target gene for targeting by a zinc finger protein. Some of the methods of target site selection seek to identify one or more target segments having a DNA motif containing one or more so-called D-able subsites having the sequence 5′NNGK3′. Other methods of the invention are directed to selection of target segments within target genes using a correspondence regime between different triplets of three bases and the three possible positions of a triplet within a nine-base site. In another aspect, the invention provides methods of designing zinc finger proteins that bind to a preselected target site. These methods can be used following the preselection of target sites according to the procedures and criteria described above. The methods of design use a database containing information about previously characterized zinc finger proteins.

    摘要翻译： 本发明提供了从靶基因中选择锌指蛋白靶向的最佳亚序列的标准和方法。 靶位点选择的一些方法寻求鉴定具有含有一个或多个具有序列5'NNGK3'的一个或多个所谓的D能子集的DNA基序的一个或多个靶区段。 本发明的其它方法涉及使用三个碱基的不同三联体之间的对应方式和九个碱基位点内三联体的三个可能位置来选择靶基因内的靶区段。 另一方面，本发明提供了设计结合预选靶位点的锌指蛋白的方法。 这些方法可以根据上述程序和标准在预选目标位点之后使用。 设计方法使用包含以前特征的锌指蛋白的信息的数据库。

    Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
    3.
    发明授权
    Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites 有权
    选择锌指蛋白靶向位点和设计锌指蛋白结合预选位点的方法

    公开(公告)号：US07788044B2

    公开(公告)日：2010-08-31

    申请号：US11707284

    申请日：2007-02-12

    申请人： Stephen P. Eisenberg ,  Casey C. Case ,  George N. Cox, III ,  Andrew Jamieson ,  Edward J. Rebar

    发明人： Stephen P. Eisenberg ,  Casey C. Case ,  George N. Cox, III ,  Andrew Jamieson ,  Edward J. Rebar

    IPC分类号： G06F19/00 ,  C12Q1/68

    CPC分类号： C12N15/1089 ,  C07K14/47 ,  C07K14/4702 ,  C12Q1/6811 ,  G01N33/5308 ,  G01N33/6875 ,  G06F19/18

    摘要： The invention provides criteria and methods for selecting optimum subsequence(s) from a target gene for targeting by a zinc finger protein. Some of the methods of target site selection seek to identify one or more target segments having a DNA motif containing one or more so-called D-able subsites having the sequence 5′NNGK3′. Other methods of the invention are directed to selection of target segments within target genes using a correspondence regime between different triplets of three bases and the three possible positions of a triplet within a nine-base site. In another aspect, the invention provides methods of designing zinc finger proteins that bind to a preselected target site. These methods can be used following the preselection of target sites according to the procedures and criteria described above. The methods of design use a database containing information about previously characterized zinc finger proteins.

    摘要翻译： 本发明提供了从靶基因中选择锌指蛋白靶向的最佳亚序列的标准和方法。 靶位点选择的一些方法寻求鉴定具有含有一个或多个具有序列5'NNGK3'的一个或多个所谓的D能子集的DNA基序的一个或多个靶区段。 本发明的其它方法涉及使用三个碱基的不同三联体之间的对应方式和九个碱基位点内三联体的三个可能位置来选择靶基因内的靶区段。 另一方面，本发明提供了设计结合预选靶位点的锌指蛋白的方法。 这些方法可以根据上述程序和标准在预选目标位点之后使用。 设计方法使用包含以前特征的锌指蛋白的信息的数据库。

    Regulation of angiogenesis with zinc finger proteins
    6.
    发明授权
    Regulation of angiogenesis with zinc finger proteins 失效
    用锌指蛋白调节血管生成

    公开(公告)号：US08071564B2

    公开(公告)日：2011-12-06

    申请号：US12553826

    申请日：2009-09-03

    申请人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

    发明人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

    IPC分类号： A61K48/00 ,  C07H21/02

    CPC分类号： A61K48/005 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/52 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2799/021 ,  C12N2810/85 ,  C12N2830/008

    摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

    摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。

    Regulation of angiogenesis with zinc finger proteins
    8.
    发明授权
    Regulation of angiogenesis with zinc finger proteins 失效
    用锌指蛋白调节血管生成

    公开(公告)号：US07560440B2

    公开(公告)日：2009-07-14

    申请号：US11486994

    申请日：2006-07-14

    申请人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

    发明人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

    IPC分类号： A61K48/00

    CPC分类号： A61K48/005 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/52 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2799/021 ,  C12N2810/85 ,  C12N2830/008

    摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

    摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。

    REGULATION OF ANGIOGENESIS WITH ZINC FINGER PROTEINS
    9.
    发明申请
    REGULATION OF ANGIOGENESIS WITH ZINC FINGER PROTEINS 失效
    用锌指纹蛋白调节血管生成

    公开(公告)号：US20100093838A1

    公开(公告)日：2010-04-15

    申请号：US12553826

    申请日：2009-09-03

    申请人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

    发明人： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

    IPC分类号： A61K31/7088 ,  A61P9/00

    CPC分类号： A61K48/005 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/52 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2799/021 ,  C12N2810/85 ,  C12N2830/008

    摘要： Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

    摘要翻译： 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。