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13 条记录 (耗时 0.017 秒)

    METHOD AND REAGENTS TO IMPROVE NONANIMAL OCULAR TOXICITY TESTS
    1.
    发明申请
    METHOD AND REAGENTS TO IMPROVE NONANIMAL OCULAR TOXICITY TESTS 有权

    公开(公告)号：US20230081986A1

    公开(公告)日：2023-03-16

    申请号：US17889026

    申请日：2022-08-16

    申请人： Stewart Lebrun ,  Linda Nguyen

    发明人： Stewart Lebrun ,  Linda Nguyen

    IPC分类号： G01N33/50

    摘要： Disclosed are formulations and procedures to improve the accuracy of nonanimal tests. Disclosed procedures involve both the direct application of the substance to be tested to the excised eye or other suitable test matrix, such as a differentiated tissue, and the application of an aqueous layer to the apical surface and then the addition of the substance to be tested as an overlay to the aqueous layer for a period of time so as to allow metabolism of the substance to be tested by the eye or test matrix, but not so long as to result in nonirritant or nontoxic test substance resulting in a FP result.

    MATERIALS AND METHODS TO IMPROVE IN VITRO TOXICITY PREDICTIONS
    2.
    发明公开
    MATERIALS AND METHODS TO IMPROVE IN VITRO TOXICITY PREDICTIONS 审中-公开

    公开(公告)号：US20240118265A1

    公开(公告)日：2024-04-11

    申请号：US18481774

    申请日：2023-10-05

    申请人： Stewart Lebrun ,  Linda Nguyen

    发明人： Stewart Lebrun ,  Linda Nguyen

    IPC分类号： G01N33/50

    CPC分类号： G01N33/5014

    摘要： The disclosure relates to materials and methods for the in vitro testing of irritants. It was discovered that the accuracy of nonanimal toxicity tests is improved when the distance migrated on a synthetic substrate is used as a correction factor for macromolecular, cell, and specific organotypic eye toxicity test systems. The method involves applying the test substance to a solid support test material, measuring the migration distance and then using the migration distance measurement to improve on an existing nonanimal toxicity test; where the existing toxicity test prediction can be: (1) a macromolecular test, (2) a cultured epithelium-based (3) and/or an organotypic test; measuring a test system response; and multiplying, adding, or otherwise using as a correction factor, the measured migration distance, and using this to have an improved prediction of the toxicity of the test substance based on the combined response.

    Microarray-based analysis of rheumatoid arthritis markers
    3.
    发明申请
    Microarray-based analysis of rheumatoid arthritis markers 审中-公开
    类风湿关节炎标记物的基于微阵列的分析

    公开(公告)号：US20060127963A1

    公开(公告)日：2006-06-15

    申请号：US10719770

    申请日：2003-11-21

    申请人： Stewart Lebrun

    发明人： Stewart Lebrun

    IPC分类号： G01N33/537 ,  C12M1/34 ,  G01N33/53 ,  G01N33/543

    CPC分类号： C07K14/4713 ,  G01N33/564 ,  G01N2800/102

    摘要： A microarray and method of screening for rheumatoid arthritis (RA) in a mammal is disclosed. The method comprises contacting a sample from the mammal to an immobilized polypeptide or fragment thereof homologous to at least a portion of an RA marker protein, and detecting binding of an antibody from the sample to the immobilized polypeptide or fragment thereof.

    摘要翻译： 公开了一种在哺乳动物中筛选类风湿性关节炎（RA）的微阵列和方法。 该方法包括使来自哺乳动物的样品与至少一部分RA标记蛋白同源的固定化多肽或其片段接触，并检测抗体与样品与固定化多肽或其片段的结合。

    FORMULATIONS AND METHODS RELATED TO EYE IRRITATION
    4.
    发明申请
    FORMULATIONS AND METHODS RELATED TO EYE IRRITATION 有权

    公开(公告)号：US20220000838A1

    公开(公告)日：2022-01-06

    申请号：US17203467

    申请日：2021-03-16

    申请人： Stewart Lebrun ,  Roxanne Chan ,  Sara Chavez

    发明人： Stewart Lebrun ,  Roxanne Chan ,  Sara Chavez

    IPC分类号： A61K31/375 ,  A61K47/42 ,  A61K47/02 ,  A61K47/36 ,  A61P27/02 ,  A61P39/06 ,  G01N33/50

    摘要： The disclosure relates to formulations and methods for the in vitro testing of ocular irritants. It was discovered that adding an antioxidant formulation to in vitro ocular irritation tests, including for example, a biochemical ocular irritation test, a reconstituted human corneal epithelium (RhCE) ocular irritation test and an excised eye depth of injury (DoI) test, substantially reduces the rate of false positives without diminishing test sensitivity, resulting in more accurately predicting ocular irritancy of test substances. More particularly, the disclosed method employs relatively high physiologic concentrations of one or more antioxidants that are normally present in tears. In a variation, much higher concentrations of one or more antioxidants may provide protection against in vivo exposure to ocular irritants.