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公开(公告)号：US20110102888A1
公开(公告)日：2011-05-05
申请号：US12907196
申请日：2010-10-19
申请人： Susumu Honda , Tatsuo Nakata
发明人： Susumu Honda , Tatsuo Nakata
CPC分类号： G02B21/088 , G02B21/06 , G02B21/16 , G02B21/32
摘要： Provided is a microscope that allows irradiation with uniform illumination light without decreasing the amount of light. Employed is a microscope 1 including an incoherent light source 31 that emits incoherent light I; an optical fiber 35 on which the incoherent light I is incident and which guides the incident incoherent light I by repeated total reflection; a DMD 37 having an array of movable micromirrors each reflecting or transmitting the guided incoherent light I; an objective lens 18 that irradiates a specimen 19 with the incoherent light I reflected or transmitted by the DMD 37 and that collects fluorescence F coming from the specimen 19; a dichroic mirror 17 that splits off the collected fluorescence F coming from the specimen 19 from the incoherent light I; and a CCD camera 13 that is disposed at a position conjugate to the position of the DMD 37 and that detects the fluorescence F coming from the specimen 19 and split off by the dichroic mirror 17.
摘要翻译： 本发明提供一种能够在不减少光量的情况下照射均匀的照明光的显微镜。 使用的显微镜1包括发出非相干光I的非相干光源31; 其中非相干光I入射的光纤35并且通过重复的全反射来引导入射的非相干光I; 具有可移动微镜阵列的DMD37，每个反射或透射被引导的非相干光I; 对由样品19照射由DMD37反射或透射的非相干光I并收集来自样品19的荧光F的物镜18; 分离反射镜17，其分离来自样品19的不相干光I的所收集的荧光F; 以及CCD摄像机13，其设置在与DMD37的位置共轭的位置，并且检测来自检体19的荧光F，并由分色镜17分离。
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公开(公告)号：US07483131B2
公开(公告)日：2009-01-27
申请号：US11799196
申请日：2007-05-01
申请人： Nobuhiro Takamizawa , Tatsuo Nakata
发明人： Nobuhiro Takamizawa , Tatsuo Nakata
IPC分类号： G01J3/30
CPC分类号： G02B21/0076 , G02B21/0032 , G02B21/006
摘要： An observation laser beam for observing a specimen and a manipulation laser beam for manipulating the specimen are multiplexed; the multiplexed beams irradiate the specimen, which is mounted on a stage, via an objective lens; and fluorescence emitted from inside the specimen in the observation optical axis direction is detected. In a preparation mode, a focal-position adjusting unit is controlled so that the focal position of the observation laser beam and the focal position of the manipulation laser beam are coincident, independent of the movement of the objective lens or the stage by a focusing mechanism; and in an observation mode, the focal-position adjusting unit is controlled so as to cancel out the shift of the focal position of the manipulation laser beam according to the movement of the objective lens or the stage.
摘要翻译： 用于观察样本的观察激光束和用于操纵样本的操纵激光束被多路复用; 复用光束经由物镜照射安装在台架上的试样; 并且在观察光轴方向检测从样本内部发出的荧光。 在准备模式中，控制焦点位置调节单元，使得观察激光束的焦点位置和操纵激光束的焦点位置重合，而与物镜或舞台的移动无关，由聚焦机构 ; 并且在观察模式中，控制焦点位置调整单元，以便根据物镜或舞台的移动抵消操纵激光束的焦点位置的偏移。
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3.发明授权Laser scanning microscope having a mechanism which prevents stimulation light from reaching a light detector 有权具有防止刺激光到达光检测器的机构的激光扫描显微镜公开(公告)号：US07417211B2
公开(公告)日：2008-08-26
申请号：US11252564
申请日：2005-10-18
申请人： Tatsuo Nakata , Masahiro Oba
发明人： Tatsuo Nakata , Masahiro Oba
CPC分类号： G02B21/0064 , G02B21/0076 , G02B21/0084
摘要： A laser scanning microscope which focuses light beams from a laser beam source to a sample by means of an objective lens and detects transmission light from the sample, reflection light, or fluorescence generated from the sample, includes an observation laser scanning optical system which irradiates coherent light from one side of the sample and which carries out scanning the sample, a stimulation laser scanning optical system which irradiates coherent light from an opposite side across the sample and which carries out scanning the sample, an observation light detector provided to be branched from the observation laser scanning optical system, and a light invasion preventing section which prevents the coherent light irradiated from the stimulation laser scanning optical system from invading the observation light detector.
摘要翻译： 一种激光扫描显微镜，其将来自激光束源的光束通过物镜聚焦到样品，并检测来自样品的透射光，反射光或从样品产生的荧光，其包括观察激光扫描光学系统，其照射相干 来自样品一侧的光并进行扫描样品的激光激光扫描光学系统，其对来自相反侧的相干光照射到样品上并执行扫描样品;观察光检测器，其从 观察激光扫描光学系统和防止从刺激激光扫描光学系统照射的相干光侵入观察光检测器的光入射防止部。
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公开(公告)号：US07196843B2
公开(公告)日：2007-03-27
申请号：US10393721
申请日：2003-03-21
申请人： Tatsuo Nakata
发明人： Tatsuo Nakata
CPC分类号： G02B21/06 , G02B21/0052 , G02B21/0072 , G02B21/0076 , G02B21/008 , G02B21/16 , G02B21/24 , G02B21/32
摘要： A confocal microscope apparatus comprises a first optical scanning system which obtains a scan image of a sample using a laser beam from a first laser light source, a second optical scanning system which scans specific regions of a sample with a laser beam from a second laser light source that is different from the first laser light source, thereby causing a particular phenomenon, and a beam diameter varying mechanism which can change the beam diameter of the laser beam of at least one of the first optical scanning system and the second optical scanning system. With this configuration, the apparatus further comprises an excitation light intensity distribution calculator which calculates and stores the excitation light intensity distribution along a depth direction on the sample surface from the beam diameter of the laser beam output from the beam diameter varying mechanism.
摘要翻译： 共聚焦显微镜装置包括：第一光学扫描系统，其使用来自第一激光光源的激光束获得样品的扫描图像;第二光学扫描系统，其利用来自第二激光的激光束扫描样品的特定区域 源，其不同于第一激光光源，从而引起特定现象，以及光束直径改变机构，其可以改变第一光学扫描系统和第二光学扫描系统中的至少一个的激光束的光束直径。 利用该结构，该装置还包括：激发光强度分布计算器，其根据从光束直径变化机构输出的激光束的光束直径，沿着深度方向在样品表面上计算和存储激发光强度分布。
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公开(公告)号：US07176428B2
公开(公告)日：2007-02-13
申请号：US11070191
申请日：2005-03-03
CPC分类号： G01N21/6486 , G01N21/6456
摘要： The invention provides a multiphoton-excitation-type examination apparatus that efficiently generates a multiphoton-excitation effect, that makes the measurement head compact, and that can be easily adjusted when the measurement head is replaced. The multiphoton-excitation-type examination apparatus comprises a laser light source that oscillates ultrashort pulsed laser light; an optical fiber that transmits the ultrashort pulsed laser light from the laser light source; a support member; a measurement head supported on the support member so as to be movable upwards and downwards and at an angle, and having an optical system that irradiates a specimen with the ultrashort pulsed laser light transmitted by the optical fiber that measures fluorescence or reflected light coming from the specimen; and a dispersion-compensating member, in the measurement head, that compensates for group velocity dispersion of the ultrashort pulsed laser light irradiated onto the specimen.
摘要翻译： 本发明提供了一种多光子激发型检查装置，其有效地产生使得测量头紧凑，并且在更换测量头时可以容易地调节的多光子激发效果。 多光子激发型检查装置包括：振荡超短脉冲激光的激光光源; 从激光光源发射超短脉冲激光的光纤; 支撑构件; 测量头支撑在支撑构件上，以便可上下移动一定角度，并且具有光学系统，该光学系统用由光纤透射的超短脉冲激光照射样本，该光纤测量荧光或来自 标本; 以及在测量头中补偿照射到样本上的超短脉冲激光的组速度色散的色散补偿部件。
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6.发明授权Laser scanning confocal microscope apparatus, image recording method, and recording medium 有权激光扫描共聚焦显微镜装置，图像记录方法和记录介质公开(公告)号：US07158294B2
公开(公告)日：2007-01-02
申请号：US10310657
申请日：2002-12-05
申请人： Shinji Motomura , Tatsuo Nakata
发明人： Shinji Motomura , Tatsuo Nakata
IPC分类号： G02B21/06
CPC分类号： G02B21/0076 , G02B21/0084
摘要： An image recording method for use in a confocal laser scanning microscope apparatus is configured to scan a specimen with each of a plurality of laser lights at least having different wavelengths as spotlight, to detect the light from the specimen based on the spotlight, and to partition and record obtained image information.
摘要翻译： 在共焦激光扫描显微镜装置中使用的图像记录方法被配置为使用至少具有不同波长的多个激光中的每一个作为聚光灯来扫描样本，以基于聚光灯检测来自样本的光，并且分割 并记录获得的图像信息。
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公开(公告)号：US08531762B2
公开(公告)日：2013-09-10
申请号：US13283025
申请日：2011-10-27
申请人： Tatsuo Nakata
发明人： Tatsuo Nakata
CPC分类号： G02B21/06 , G02B21/0052 , G02B21/0072 , G02B21/0076 , G02B21/008 , G02B21/16 , G02B21/24 , G02B21/32
摘要： A confocal microscope apparatus comprises a first optical scanning system which obtains a scan image of a sample using a laser beam from a first laser light source, a second optical scanning system which scans specific regions of a sample with a laser beam from a second laser light source that is different from the first laser light source, thereby causing a particular phenomenon, and a beam diameter varying mechanism which can change the beam diameter of the laser beam of at least one of the first optical scanning system and the second optical scanning system. With this configuration, the apparatus further comprises an excitation light intensity distribution calculator which calculates and stores the excitation light intensity distribution along a depth direction on the sample surface from the beam diameter of the laser beam output from the beam diameter varying mechanism.
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公开(公告)号：US08445865B2
公开(公告)日：2013-05-21
申请号：US12784643
申请日：2010-05-21
申请人： Hiroshi Sasaki , Tatsuo Nakata
发明人： Hiroshi Sasaki , Tatsuo Nakata
IPC分类号： G01J1/58
CPC分类号： G02B21/0076
摘要： A scanning microscope device includes a light source that emits laser light; an X-Y galvanometer mirror that scans the laser light on a sample; an objective lens that irradiates the sample with the scanned laser light and collects fluorescence generated at an irradiated position; a non-descan-detection excitation DM that is disposed between the X-Y galvanometer mirror and the objective lens and separates the laser light and the fluorescence from each other; a fiber that receives the separated fluorescence through an entrance end thereof and emits the fluorescence from an exit end thereof that is formed in a substantially linear shape; a diffraction grating that disperses the fluorescence emitted from the exit end of the fiber in a direction orthogonal to a longitudinal direction of the exit end; and a multi-anode PMT having plural cells arrayed in the dispersing direction of the dispersed fluorescence.
摘要翻译： 扫描显微镜装置包括发射激光的光源; 用于扫描样品上的激光的X-Y电流计镜; 物镜，用扫描的激光照射样品并收集在照射位置产生的荧光; 设置在X-Y电流计反射镜和物镜之间并将激光和荧光彼此分离的非除数检测激励DM; 通过其入口端接收分离的荧光并从其出口端发射形成大致线状的荧光的光纤; 衍射光栅，其在与所述出射端的长度方向正交的方向上分散从所述光纤的出射端发射的荧光; 以及具有沿分散荧光的分散方向排列的多个单元的多阳极PMT。
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公开(公告)号：US07876500B2
公开(公告)日：2011-01-25
申请号：US12059043
申请日：2008-03-31
IPC分类号： G02B21/00
CPC分类号： G02B21/367
摘要： It is possible to check for observation success or failure and the observation history without waiting for observation to be completely finished, thus saving time and energy required for observation, and avoiding lost opportunities for observation of precious samples etc. Provided is a microscope apparatus including an image acquisition unit for acquiring a plurality of frame images while varying a plurality of parameters; an image saving unit for successively saving the frame images acquired by the image acquisition unit; a property-information saving unit for saving property information in which identifying information of the saved frame images is associated with the parameters; and a control unit for controlling these units, wherein the control unit saves updated property information in the property-information saving unit each time the frame image is saved in the image saving unit.
摘要翻译： 可以检查观察成功或失败以及观察历史，而不等待观察完全完成，从而节省观察所需的时间和精力，并避免丢失观察珍贵样品等的机会。提供一种显微镜装置，包括 图像获取单元，用于在改变多个参数的同时获取多个帧图像; 图像保存单元，用于连续地保存由图像获取单元获取的帧图像; 属性信息保存单元，用于保存其中保存的帧图像的识别信息与参数相关联的属性信息; 以及用于控制这些单元的控制单元，其中，每当帧图像被保存在图像保存单元中时，控制单元将更新的属性信息保存在属性信息保存单元中。
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公开(公告)号：US07675676B2
公开(公告)日：2010-03-09
申请号：US11677444
申请日：2007-02-21
申请人： Tatsuo Nakata
发明人： Tatsuo Nakata
CPC分类号： G02B21/06 , G02B21/0052 , G02B21/0072 , G02B21/0076 , G02B21/008 , G02B21/16 , G02B21/24 , G02B21/32
摘要： A confocal microscope apparatus comprises a first optical scanning system which obtains a scan image of a sample using a laser beam from a first laser light source, a second optical scanning system which scans specific regions of a sample with a laser beam from a second laser light source that is different from the first laser light source, thereby causing a particular phenomenon, and a beam diameter varying mechanism which can change the beam diameter of the laser beam of at least one of the first optical scanning system and the second optical scanning system. With this configuration, the apparatus further comprises an excitation light intensity distribution calculator which calculates and stores the excitation light intensity distribution along a depth direction on the sample surface from the beam diameter of the laser beam output from the beam diameter varying mechanism.
摘要翻译： 共聚焦显微镜装置包括：第一光学扫描系统，其使用来自第一激光光源的激光束获得样品的扫描图像;第二光学扫描系统，其利用来自第二激光的激光束扫描样品的特定区域 源，其不同于第一激光光源，从而引起特定现象，以及光束直径改变机构，其可以改变第一光学扫描系统和第二光学扫描系统中的至少一个的激光束的光束直径。 利用该结构，该装置还包括：激发光强度分布计算器，其根据从光束直径变化机构输出的激光束的光束直径，沿着深度方向在样品表面上计算和存储激发光强度分布。
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