公开(公告)号：US07417211B2

公开(公告)日：2008-08-26

申请号：US11252564

申请日：2005-10-18

申请人： Tatsuo Nakata ,  Masahiro Oba

发明人： Tatsuo Nakata ,  Masahiro Oba

IPC分类号： G02B7/04 ,  G02B21/00 ,  F21V9/16

CPC分类号： G02B21/0064 ,  G02B21/0076 ,  G02B21/0084

摘要： A laser scanning microscope which focuses light beams from a laser beam source to a sample by means of an objective lens and detects transmission light from the sample, reflection light, or fluorescence generated from the sample, includes an observation laser scanning optical system which irradiates coherent light from one side of the sample and which carries out scanning the sample, a stimulation laser scanning optical system which irradiates coherent light from an opposite side across the sample and which carries out scanning the sample, an observation light detector provided to be branched from the observation laser scanning optical system, and a light invasion preventing section which prevents the coherent light irradiated from the stimulation laser scanning optical system from invading the observation light detector.

摘要翻译： 一种激光扫描显微镜，其将来自激光束源的光束通过物镜聚焦到样品，并检测来自样品的透射光，反射光或从样品产生的荧光，其包括观察激光扫描光学系统，其照射相干 来自样品一侧的光并进行扫描样品的激光激光扫描光学系统，其对来自相反侧的相干光照射到样品上并执行扫描样品;观察光检测器，其从 观察激光扫描光学系统和防止从刺激激光扫描光学系统照射的相干光侵入观察光检测器的光入射防止部。

公开(公告)号：US20060086887A1

公开(公告)日：2006-04-27

申请号：US11252564

申请日：2005-10-18

申请人： Tatsuo Nakata ,  Masahiro Oba

发明人： Tatsuo Nakata ,  Masahiro Oba

IPC分类号： G02B27/40 ,  H01J40/14 ,  G02B27/64

CPC分类号： G02B21/0064 ,  G02B21/0076 ,  G02B21/0084

摘要： A laser scanning microscope which focuses light beams from a laser beam source to a sample by means of an objective lens and detects transmission light from the sample, reflection light, or fluorescence generated from the sample, includes an observation laser scanning optical system which irradiates coherent light from one side of the sample and which carries out scanning the sample, a stimulation laser scanning optical system which irradiates coherent light from an opposite side across the sample and which carries out scanning the sample, an observation light detector provided to be branched from the observation laser scanning optical system, and a light invasion preventing section which prevents the coherent light irradiated from the stimulation laser scanning optical system from invading the observation light detector.

摘要翻译： 一种激光扫描显微镜，其将来自激光束源的光束通过物镜聚焦到样品，并检测来自样品的透射光，反射光或从样品产生的荧光，其包括观察激光扫描光学系统，其照射相干 来自样品一侧的光并进行扫描样品的激光激光扫描光学系统，其对来自相反侧的相干光照射到样品上并执行扫描样品;观察光检测器，其从 观察激光扫描光学系统和防止从刺激激光扫描光学系统照射的相干光侵入观察光检测器的光入射防止部。

公开(公告)号：US07804642B2

公开(公告)日：2010-09-28

申请号：US11913833

申请日：2006-05-16

申请人： Nobuyuki Nagasawa ,  Masahiro Oba ,  Tetsuya Koike ,  Yoshihisa Tanikawa ,  Yujin Arai

发明人： Nobuyuki Nagasawa ,  Masahiro Oba ,  Tetsuya Koike ,  Yoshihisa Tanikawa ,  Yujin Arai

IPC分类号： G02B21/06

CPC分类号： G02B21/002 ,  G02B21/008 ,  G02B21/365

摘要： A scanning examination apparatus 1 is provided, the apparatus including a detachable objective lens 6 or 6′, a scanner 3 for two-dimensionally scanning light F from a specimen 100 focused by the objective lens 6 or 6′, a scanner control device 13 for controlling the operation of the scanner 3, and an optical detector 9 for detecting light scanned by the scanner 3, wherein the scanner control device 13 changes the scanning direction of the light F depending on an image formation mode of the light F at the objective lens 6 or 6′ which is attached.

摘要翻译： 提供了一种扫描检查装置1，该装置包括一个可拆卸的物镜6或6'，用于从由物镜6或6'聚焦的样本100二维地扫描光F的扫描器3，用于 控制扫描仪3的操作，以及用于检测由扫描器3扫描的光的光学检测器9，其中扫描仪控制装置13根据物镜上的光F的图像形成模式改变光F的扫描方向 6或6'。

公开(公告)号：US08786720B2

公开(公告)日：2014-07-22

申请号：US12332540

申请日：2008-12-11

申请人： Masahiro Oba ,  Nobuyuki Nagasawa

发明人： Masahiro Oba ,  Nobuyuki Nagasawa

IPC分类号： H04N5/235

CPC分类号： G06K9/20 ,  G06T5/50 ,  G06T7/0012 ,  G06T2207/20221 ,  H04N5/23232 ,  H04N5/2352

摘要： When a biological specimen, such as living cells, biological tissue, or a small animal, is quantitatively observed, the specimen is observed for a long time or over a long period, while maintaining the quantitative properties. Provided is an observation method of observing luminescence or fluorescence emitted from a biological specimen. The observation method includes a referring step of referring to image data previously acquired; an intensity determining step of determining whether the intensity of the image data previously acquired is saturated; an exposure dividing step of dividing the exposure of the image data previously acquired one time into a plurality of exposures when the intensity is determined as being saturated in the intensity determining step; an image acquiring step of acquiring images the same number of times as the number of divided exposures; and an integrating step of integrating the images separately acquired multiple times into a single image.

摘要翻译： 当定量观察诸如活细胞，生物组织或小动物的生物样本时，长时间或长时间观察样本，同时保持定量性质。 提供了观察从生物样本发出的发光或荧光的观察方法。 观察方法包括参照先前获取的图像数据的参考步骤; 强度确定步骤，确定先前获取的图像数据的强度是否饱和; 曝光分割步骤，当在所述强度确定步骤中确定所述强度被确定为饱和时，将先前获取的一次的图像数据的曝光除以多个曝光; 图像获取步骤，获取与分割曝光数相同次数的图像; 以及将多次分开获取的图像集成到单个图像中的整合步骤。

公开(公告)号：US20100309547A1

公开(公告)日：2010-12-09

申请号：US12790112

申请日：2010-05-28

申请人： Tadashi HIRATA ,  Masahiro Oba

发明人： Tadashi HIRATA ,  Masahiro Oba

IPC分类号： G02B21/02

CPC分类号： G02B21/0012 ,  A61B1/00154 ,  A61B1/313 ,  A61B1/32 ,  A61B2503/40 ,  G01N21/01 ,  G02B21/02 ,  G02B21/248

摘要： An objective-optical-system positioning apparatus includes a positioning unit interposed between an objective optical system having a small-diameter end section and an examination optical system for examining light collected by the objective optical system; and a substantially cylindrical support unit, one end of which is secured to an organism, and which internally supports the small-diameter end section in a detachable manner. The positioning unit includes a holding part for holding the objective optical system and a moving mechanism supporting the holding part such that the holding part is freely movable in a direction intersecting with a direction of an optical axis of the objective optical system, and the other end of the support unit has a tapered inner surface whose diameter gradually increases toward the tip.

摘要翻译： 一种物镜光学系统定位装置，包括：位于具有小直径端部的物镜光学系统和用于检查物镜光学系统收集的光的检查光学系统之间的定位单元; 以及一个基本上圆柱形的支撑单元，其一端固定到生物体上，并以可拆卸的方式内部支撑该小直径端部。 定位单元包括用于保持物镜光学系统的保持部件和支撑保持部件的移动机构，使得保持部件能够在与物镜光学系统的光轴方向相交的方向上自由移动，另一端 的支撑单元具有锥形内表面，其直径朝着尖端逐渐增加。

公开(公告)号：US08405903B2

公开(公告)日：2013-03-26

申请号：US12790112

申请日：2010-05-28

申请人： Tadashi Hirata ,  Masahiro Oba

发明人： Tadashi Hirata ,  Masahiro Oba

IPC分类号： G02B21/00 ,  G02B21/02

CPC分类号： G02B21/0012 ,  A61B1/00154 ,  A61B1/313 ,  A61B1/32 ,  A61B2503/40 ,  G01N21/01 ,  G02B21/02 ,  G02B21/248

摘要： An objective-optical-system positioning apparatus includes a positioning unit interposed between an objective optical system having a small-diameter end section and an examination optical system for examining light collected by the objective optical system; and a substantially cylindrical support unit, one end of which is secured to an organism, and which internally supports the small-diameter end section in a detachable manner. The positioning unit includes a holding part for holding the objective optical system and a moving mechanism supporting the holding part such that the holding part is freely movable in a direction intersecting with a direction of an optical axis of the objective optical system, and the other end of the support unit has a tapered inner surface whose diameter gradually increases toward the tip.

摘要翻译： 一种物镜光学系统定位装置，包括：位于具有小直径端部的物镜光学系统和用于检查物镜光学系统收集的光的检查光学系统之间的定位单元; 以及一个基本上圆柱形的支撑单元，其一端固定到生物体上，并以可拆卸的方式内部支撑该小直径端部。 定位单元包括用于保持物镜光学系统的保持部件和支撑保持部件的移动机构，使得保持部件能够在与物镜光学系统的光轴方向相交的方向上自由移动，另一端 的支撑单元具有锥形内表面，其直径朝着尖端逐渐增加。

公开(公告)号：US20090073554A1

公开(公告)日：2009-03-19

申请号：US11913833

申请日：2006-05-16

申请人： Nobuyuki Nagasawa ,  Masahiro Oba ,  Tetsuya Koike ,  Yoshihisa Tanikawa ,  Yujin Arai

发明人： Nobuyuki Nagasawa ,  Masahiro Oba ,  Tetsuya Koike ,  Yoshihisa Tanikawa ,  Yujin Arai

IPC分类号： G02B21/06

CPC分类号： G02B21/002 ,  G02B21/008 ,  G02B21/365

摘要： A scanning examination apparatus 1 is provided, the apparatus including a detachable objective lens 6 or 6′, a scanner 3 for two-dimensionally scanning light F from a specimen 100 focused by the objective lens 6 or 6′, a scanner control device 13 for controlling the operation of the scanner 3, and an optical detector 9 for detecting light scanned by the scanner 3, wherein the scanner control device 13 changes the scanning direction of the light F depending on an image formation mode of the light F at the objective lens 6 or 6′ which is attached.

摘要翻译： 提供了一种扫描检查装置1，该装置包括一个可拆卸的物镜6或6'，用于从由物镜6或6'聚焦的样本100二维地扫描光F的扫描器3，用于 控制扫描仪3的操作，以及用于检测由扫描器3扫描的光的光学检测器9，其中扫描仪控制装置13根据物镜上的光F的图像形成模式改变光F的扫描方向 6或6'。

公开(公告)号：US07477775B2

公开(公告)日：2009-01-13

申请号：US10883340

申请日：2004-07-01

申请人： Masahiro Oba ,  Tetsuya Koike

发明人： Masahiro Oba ,  Tetsuya Koike

IPC分类号： G06K9/00

CPC分类号： G02B21/006 ,  G02B21/0024 ,  G02B21/008 ,  G06K9/00127

摘要： A CPU executes a process for building an image, which is projected on three planes such as XY, YZ, and XZ planes mutually intersecting in three-dimensional space, of a three-dimensional image obtained by stacking in a direction of an optical axis a plurality of two-dimensional confocal images, which are obtained by changing the focal position of an objective lens against a sample in the direction of the optical axis of the objective lens. Then, a target region implemented by integrating pixels, whose points projected on the three planes are included in regions respectively specified for the three planes, among pixels configuring each of the two-dimensional confocal image.

摘要翻译： CPU执行用于构建图像的处理，该图像投影在三维空间中相互相交的三维平面（例如XY，YZ和XZ平面）上，通过沿光轴a的方向堆叠获得的三维图像 多个二维共焦图像，其通过在物镜的光轴方向上相对于样本改变物镜的焦点位置而获得。 然后，在构成每个二维共焦图像的像素中，通过对投影在三个平面上的点进行积分的像素来实现的目标区域被包括在分别为三个平面指定的区域中。

公开(公告)号：US20080253968A1

公开(公告)日：2008-10-16

申请号：US12100078

申请日：2008-04-09

申请人： Masahiro Oba ,  Nobuhiko Onda

发明人： Masahiro Oba ,  Nobuhiko Onda

IPC分类号： A61K49/00

CPC分类号： G01N21/6456 ,  G01N21/6428

摘要： It is possible to perform quantitative examination of biological tissue even with a solution composed of a near-infrared fluorescent dye and a polypeptide. An image acquired before administering a reagent is subtracted from an image acquired after administering the reagent to remove regions appearing in both images, such as autofluorescence, thus obtaining an image in which only detected fluorescence affected by administration of the reagent is extracted. Because tumors have many (leaky) blood vessels, regions having many areas of high intensity in the extracted image can be recognized as tumors.

摘要翻译： 即使用近红外荧光染料和多肽组成的溶液，也可以对生物组织进行定量检查。 从施用试剂后获取的图像中减去在施用试剂之前获取的图像以除去两个图像中出现的区域，例如自发荧光，从而获得其中仅提取检测到的受试剂施用影响的荧光的图像。 因为肿瘤有许多（泄漏）血管，所以在提取的图像中具有许多高强度区域的区域可被认为是肿瘤。

公开(公告)号：US20050013478A1

公开(公告)日：2005-01-20

申请号：US10883340

申请日：2004-07-01

申请人： Masahiro Oba ,  Tetsuya Koike

发明人： Masahiro Oba ,  Tetsuya Koike

IPC分类号： G02B21/00 ,  G06K9/00

CPC分类号： G02B21/006 ,  G02B21/0024 ,  G02B21/008 ,  G06K9/00127

摘要： A CPU executes a process for building an image, which is projected on three planes such as XY, YZ, and XZ planes mutually intersecting in three-dimensional space, of a three-dimensional image obtained by stacking in a direction of an optical axis a plurality of two-dimensional confocal images, which are obtained by changing the focal position of an objective lens against a sample in the direction of the optical axis of the objective lens. Then, a target region implemented by integrating pixels, whose points projected on the three planes are included in regions respectively specified for the three planes, among pixels configuring each of the two-dimensional confocal image.

摘要翻译： CPU执行用于构建图像的处理，该图像投影在三维空间中相互相交的三维平面（例如XY，YZ和XZ平面）上，通过沿光轴a的方向堆叠获得的三维图像 多个二维共焦图像，其通过在物镜的光轴方向上相对于样本改变物镜的焦点位置而获得。 然后，在构成每个二维共焦图像的像素中，通过对投影在三个平面上的点进行积分的像素来实现的目标区域被包括在分别为三个平面指定的区域中。