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    Nucleic acid molecule having binding affinity to a target molecule and a method for generating the same
    1.
    发明授权
    Nucleic acid molecule having binding affinity to a target molecule and a method for generating the same 有权

    公开(公告)号：US09976145B2

    公开(公告)日：2018-05-22

    申请号：US13978804

    申请日：2012-01-10

    申请人： Florian Jarosch ,  Sven Klussmann ,  Simone Sell ,  Werner Purschke ,  Christian Maasch ,  Axel Vater ,  Kai Hohlig

    发明人： Florian Jarosch ,  Sven Klussmann ,  Simone Sell ,  Werner Purschke ,  Christian Maasch ,  Axel Vater ,  Kai Hohlig

    IPC分类号： C12N15/115 ,  C12N15/11 ,  G01N33/53 ,  C12N15/10

    CPC分类号： C12N15/115 ,  C12N15/102 ,  C12N15/111 ,  C12N2310/16 ,  C12N2310/32 ,  C12N2320/13 ,  C12N2330/31 ,  G01N33/5308 ,  G01N2400/10 ,  G01N2500/04

    摘要： The present invention is related to a method for generating a nucleic acid molecule capable of binding to a target molecule comprising the following steps: a) providing a reference nucleic acid molecule, wherein the reference nucleic acid molecule is capable of binding to the target molecule and wherein the reference nucleic acid molecule comprises a sequence of nucleotides, wherein the sequence of nucleotides comprises n nucleotides; b) preparing a first level derivative of the reference nucleic acid molecule, wherein the first level derivative of the reference nucleic acid molecule differs from the reference nucleic acid molecule at one nucleotide position, wherein the first level derivative is prepared by replacing the ribonucleotide at the one nucleotide position by a 2′-deoxyribonucleotide in case the reference nucleic acid has a ribonucleotide at the nucleotide position and wherein the first level derivative is prepared by replacing the 2′-deoxyribonucleotide at the one nucleotide position by a ribonucleotide in case the reference nucleic acid has a 2′-deoxyribonucleotide at the nucleotide position and wherein the nucleotide position at which the replacement is made is the modified nucleotide position; and c) repeating step b) for each nucleotide position of the reference nucleic acid molecule, thus preparing a group of first level derivatives of the reference nucleic acid molecule, wherein the group of first level derivatives of the reference nucleic acid molecule consists of n first level derivatives, wherein each of the first level derivatives of the reference nucleic acid molecule differs from the reference nucleic acid molecule by a single nucleotide replacement and wherein each of the first level derivatives of the reference nucleic acid molecule has a single modified nucleotide position which is different from the single modified nucleotide of all of the single modified nucleotide positions of the other first level derivatives of the group of first level derivatives of the reference nucleic acid molecule.

    Nucleic acid molecule having binding affinity to a target molecule and a method for generating the same
    2.
    发明申请
    Nucleic acid molecule having binding affinity to a target molecule and a method for generating the same 有权

    公开(公告)号：US20140350088A1

    公开(公告)日：2014-11-27

    申请号：US13978804

    申请日：2012-01-10

    申请人： Florian Jarosch ,  Sven Klussmann ,  Simone Sell ,  Werner Purschke ,  Christian Maasch ,  Axel Vater ,  Kai Hohlig

    发明人： Florian Jarosch ,  Sven Klussmann ,  Simone Sell ,  Werner Purschke ,  Christian Maasch ,  Axel Vater ,  Kai Hohlig

    IPC分类号： C12N15/115 ,  G01N33/53 ,  C12N15/10

    CPC分类号： C12N15/115 ,  C12N15/102 ,  C12N15/111 ,  C12N2310/16 ,  C12N2310/32 ,  C12N2320/13 ,  C12N2330/31 ,  G01N33/5308 ,  G01N2400/10 ,  G01N2500/04

    摘要： The present invention is related to a method for generating a nucleic acid molecule capable of binding to a target molecule comprising the following steps: a) providing a reference nucleic acid molecule, wherein the reference nucleic acid molecule is capable of binding to the target molecule and wherein the reference nucleic acid molecule comprises a sequence of nucleotides, wherein the sequence of nucleotides comprises n nucleotides; b) preparing a first level derivative of the reference nucleic acid molecule, wherein the first level derivative of the reference nucleic acid molecule differs from the reference nucleic acid molecule at one nucleotide position, wherein the first level derivative is prepared by replacing the ribonucleotide at the one nucleotide position by a 2′-deoxyribonucleotide in case the reference nucleic acid has a ribonucleotide at the nucleotide position and wherein the first level derivative is prepared by replacing the 2′-deoxyribonucleotide at the one nucleotide position by a ribonucleotide in case the reference nucleic acid has a 2′-deoxyribonucleotide at the nucleotide position and wherein the nucleotide position at which the replacement is made is the modified nucleotide position; and c) repeating step b) for each nucleotide position of the reference nucleic acid molecule, thus preparing a group of first level derivatives of the reference nucleic acid molecule, wherein the group of first level derivatives of the reference nucleic acid molecule consists of n first level derivatives, wherein each of the first level derivatives of the reference nucleic acid molecule differs from the reference nucleic acid molecule by a single nucleotide replacement and wherein each of the first level derivatives of the reference nucleic acid molecule has a single modified nucleotide position which is different from the single modified nucleotide of all of the single modified nucleotide positions of the other first level derivatives of the group of first level derivatives of the reference nucleic acid molecule.

    Method for Synthesizing Nucleic Acids, and Application Thereof
    3.
    发明申请
    Method for Synthesizing Nucleic Acids, and Application Thereof 审中-公开
    合成核酸的方法及其应用

    公开(公告)号：US20110027794A1

    公开(公告)日：2011-02-03

    申请号：US12905910

    申请日：2010-10-15

    申请人： Sven Klussmann ,  Florian Jarosch ,  Axel Vater

    发明人： Sven Klussmann ,  Florian Jarosch ,  Axel Vater

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12N15/102 ,  C12N15/1096 ,  C12P19/34

    摘要： The invention relates to a method for synthesizing a nucleic acid containing modified nucleotides. The method encompasses the following steps: a matrix strand is provided; —a primer which at least partially hybridizes on the matrix strand is provided; —nucleoside triphosphates, at least some of which are modified nucleoside triphosphates, are provided; —a polymerase activity is supplied; and—the matrix strand, the primer, and the nucleoside triphosphates are incubated so as to synthesize a nucleic acid that is substantially complementary to the matrix strand. The polymerase activity can be a reverse transcriptase activity.

    摘要翻译： 本发明涉及一种合成含有修饰核苷酸的核酸的方法。 该方法包括以下步骤：提供矩阵线; 提供了在基质链上至少部分杂交的引物; 核苷三磷酸盐，其中至少一些是修饰的核苷三磷酸酯; - 提供聚合酶活性; 并且将基质链，引物和核苷三磷酸盐孵育以合成基本上与基质链互补的核酸。 聚合酶活性可以是逆转录酶活性。

    Method for the Synthesizing Nucleic Acids, and Application Thereof
    5.
    发明申请
    Method for the Synthesizing Nucleic Acids, and Application Thereof 审中-公开
    合成核酸的方法及其应用

    公开(公告)号：US20080070238A1

    公开(公告)日：2008-03-20

    申请号：US10572291

    申请日：2004-09-14

    申请人： Sven Klussmann ,  Florian Jarosch ,  Axel Vater

    发明人： Sven Klussmann ,  Florian Jarosch ,  Axel Vater

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12N15/102 ,  C12N15/1096 ,  C12P19/34

    摘要： The invention relates to a method for synthesizing a nucleic acid comprising modified nucleotides. Said method encompasses the following steps: a matrix strand is provided; —a primer which at least partially hybridizes on the matrix strand is provided; —nucleoside triphosphates, at least some of which are modified nucleoside triphosphates, are provided; —a polymerase activity is supplied; and —the matrix strand, the primer, and the nucleoside triphosphates are incubated so as to synthesize a nucleic acid that is substantially complementary to the matrix strand. The inventive method is characterized in that the polymerase activity represents a reverse transcriptase activity.

    摘要翻译： 本发明涉及一种合成含有修饰核苷酸的核酸的方法。 所述方法包括以下步骤：提供矩阵线; 提供了在基质链上至少部分杂交的引物; 核苷三磷酸盐，其中至少一些是修饰的核苷三磷酸酯; - 提供聚合酶活性; 并且 - 将基质链，引物和核苷三磷酸盐孵育以合成基本上与基质链互补的核酸。 本发明的方法的特征在于聚合酶活性代表逆转录酶活性。