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公开(公告)号：US08093458B2
公开(公告)日：2012-01-10
申请号：US11631719
申请日：2005-07-07
申请人： Sylvestre Marillonnet , Carola Engler , Stefan Mühlbauer , Stefan Herz , Stefan Werner , Victor Klimyuk , Yuri Gleba
发明人： Sylvestre Marillonnet , Carola Engler , Stefan Mühlbauer , Stefan Herz , Stefan Werner , Victor Klimyuk , Yuri Gleba
CPC分类号： C12N15/8203 , C12N15/8205 , C12N15/8216 , C12N15/8257
摘要： A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: (a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an Agrobacterium strain in the presence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterologous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, (b) optionally isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.
摘要翻译： 通过从植物或植物叶中感兴趣的序列表达所述目标蛋白质来产生感兴趣的蛋白质的方法，包括：（a）通过用所述植物或所述植物叶子渗透所述植物或所述植物叶来转染所述植物或所述植物叶 农杆菌菌株在补充因子存在下，所述含有T-DNA的土壤杆菌菌株具有编码复制子的序列部分的异源DNA序列，其中编码复制子的所述序列含有所述复制子的复制子功能所必需的序列，所述序列得自 从所述复制子表达的所述感兴趣的序列，（b）任选地从所述植物或所述在步骤（a）中渗透的所述植物叶分离所述目的蛋白质，其中所述农杆菌菌株被提供有第一遗传 在没有所述补充因子的情况下，使所述农杆菌菌株对所述T-DNA转染生物有缺陷。
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公开(公告)号：US20080057563A1
公开(公告)日：2008-03-06
申请号：US11631719
申请日：2005-07-07
申请人： Sylvestre Marillonnet , Carola Engler , Stefan Muhlbauer , Stefan Herz , Stefan Werner , Victor Klimyuk , Yuri Gleba
发明人： Sylvestre Marillonnet , Carola Engler , Stefan Muhlbauer , Stefan Herz , Stefan Werner , Victor Klimyuk , Yuri Gleba
CPC分类号： C12N15/8203 , C12N15/8205 , C12N15/8216 , C12N15/8257
摘要： A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: (a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an Agrobacterium strain in the presence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterologous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, (b) isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.
摘要翻译： 通过从植物或植物叶中感兴趣的序列表达所述目标蛋白质来产生感兴趣的蛋白质的方法，包括：（a）通过用所述植物或所述植物叶子渗透所述植物或所述植物叶来转染所述植物或所述植物叶 农杆菌菌株在补充因子存在下，所述含有T-DNA的土壤杆菌菌株具有编码复制子的序列部分的异源DNA序列，其中编码复制子的所述序列含有所述复制子的复制子功能所必需的序列，所述序列得自 （b）从所述植物或所述在步骤（a）中渗透的所述植物叶分离所述感兴趣的蛋白质，其中所述农杆菌菌株被提供有第一种遗传修饰物 使得所述农杆菌菌株在缺乏所述补体因子的情况下缺陷以用所述T-DNA转染生物体。
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公开(公告)号：US20070300330A1
公开(公告)日：2007-12-27
申请号：US10586998
申请日：2005-01-19
CPC分类号： C12N15/8216
摘要： A process for replicating or for replicating and expressing a sequence of interest in a plant, comprising: (i) an RNA replicon or a precursor thereof, said RNA replicon being derived from a plus-sense single stranded RNA virus and comprising at least one sequence of interest; and (ii) a helper replicon, or a precursor thereof, wherein said helper replicon is (a) incapable of systemic movement in said plant both in the presence and in the absence of said RNA replicon (i) and (b) capable of expressing in a plant one or more proteins necessary for systemic movement of said RNA replicon (i), whereby said RNA replicon (i) is capable of replicating or replicating and expressing said sequence of interest in said plant, but unable to move systemically in said plant in the absence of said one or more proteins expressed by said helper replicon (ii).
摘要翻译： 一种在植物中复制或表达感兴趣的序列的方法，包括：（i）RNA复制子或其前体，所述RNA复制子衍生自正义单链RNA病毒，并且包含至少一个序列 出于兴趣; 辅助复制子或其前体，其中所述辅助复制子是（a）在所述RNA复制子（i）和（b）能够表达的存在和不存在下，在所述植物中不能全身运动 在植物中，所述RNA复制子（i）的系统运动所必需的一种或多种蛋白质，其中所述RNA复制子（i）能够在所述植物中复制或复制和表达所述感兴趣的序列，但不能在所述植物中系统地移动 在不存在由所述辅助复制子（ii）表达的所述一种或多种蛋白质的情况下。
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公开(公告)号：US09267143B2
公开(公告)日：2016-02-23
申请号：US10578962
申请日：2004-11-10
CPC分类号： C12N15/8203 , C12N15/8216
摘要： A process of expressing a sequence of interest in a plant, plant part, or plant cell culture, comprising: (a) providing a plant, plant part, or plant cell culture containing in cell nuclei a heterologous DNA having a sequence encoding an RNA replicon operably linked or linkable to a transcription promoter, wherein said sequence encoding an RNA replicon contains (i) sequences for replicon function of said RNA replicon, said sequences being derived from a sequence of a plant RNA virus, (ii) a sequence of interest, whereby said sequences for replicon function exhibit at selected localities of said sequences of said plant RNA virus function conservative differences from said sequence of said plant RNA virus, said differences causing an increased frequency of replicon formation compared to an RNA replicon not exhibiting said differences; and (b) causing expression of said sequence of interest.
摘要翻译： 在植物，植物部分或植物细胞培养物中表达感兴趣的序列的方法，包括：（a）提供在细胞核中含有编码RNA复制子的序列的异源DNA的植物，植物部分或植物细胞培养物 其可编码RNA复制子的所述序列包含（i）所述RNA复制子的复制子功能的序列，所述序列衍生自植物RNA病毒的序列，（ii）感兴趣的序列， 其中所述复制子功能的序列在所述植物RNA病毒的所述序列的选定位置处表现出与所述植物RNA病毒的所述序列的保守差异，所述差异与不显示所述差异的RNA复制子相比导致复制子形成的频率增加; 和（b）引起所述感兴趣序列的表达。
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公开(公告)号：US08597950B2
公开(公告)日：2013-12-03
申请号：US10586998
申请日：2005-01-19
IPC分类号： C12N15/86 , A61K38/00 , C12N7/00 , C07K14/415 , C07K14/005 , C07H21/04 , C12N5/04
CPC分类号： C12N15/8216
摘要： A process for replicating or for replicating and expressing a sequence of interest in a plant, comprising: (i) an RNA replicon or a precursor thereof, said RNA replicon being derived from a plus-sense single stranded RNA virus and comprising at least one sequence of interest; and (ii) a helper replicon, or a precursor thereof, wherein said helper replicon is (a) incapable of systemic movement in said plant both in the presence and in the absence of said RNA replicon (i) and (b) capable of expressing in a plant one or more proteins necessary for systemic movement of said RNA replicon (i), whereby said RNA replicon (i) is capable of replicating or replicating and expressing said sequence of interest in said plant, but unable to move systemically in said plant in the absence of said one or more proteins expressed by said helper replicon (ii).
摘要翻译： 一种在植物中复制或表达感兴趣的序列的方法，包括：（i）RNA复制子或其前体，所述RNA复制子衍生自正义单链RNA病毒，并且包含至少一个序列 出于兴趣; 辅助复制子或其前体，其中所述辅助复制子是（a）在所述RNA复制子（i）和（b）能够表达的存在和不存在下，在所述植物中不能全身运动 在植物中，所述RNA复制子（i）的系统运动所必需的一种或多种蛋白质，其中所述RNA复制子（i）能够在所述植物中复制或复制和表达所述感兴趣的序列，但不能在所述植物中系统地移动 在不存在由所述辅助复制子（ii）表达的所述一种或多种蛋白质的情况下。
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公开(公告)号：US08415463B2
公开(公告)日：2013-04-09
申请号：US12438743
申请日：2007-09-06
CPC分类号： C12N15/8203 , C12N15/8216 , C12N2770/26011 , C12N2770/26043
摘要： Nucleic acid comprising or encoding an RNA replica comprising, in this order, the following segments (i) to (iii): i) a nucleic acid sequence encoding a potexvirus RNA-dependent RNA polymerase or a function-conservative variant thereof; ii) a nucleic acid sequence comprising: a) a potexvirus triple gene block or a function-conservative variant thereof and b) a sequence encoding a potexviral coat protein or a function-conservative variant thereof; or a sequence encoding a tobago viral movement protein; and iii) a heterologous nucleic acid sequence expressible from said replica in a plant or in plant tissue.
摘要翻译： 包含或编码RNA复制品的核酸，其依次包括以下片段（i）至（iii）：i）编码病毒RNA依赖性RNA聚合酶或其功能保守变体的核酸序列; ii）核酸序列，其包含：a）罐状病毒三重基因嵌段或其功能保守变体，和b）编码潜在外壳蛋白或其功能保守变体的序列; 或编码多巴胺病毒运动蛋白的序列; 和iii）在植物或植物组织中可从所述复制品表达的异源核酸序列。
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公开(公告)号：US20110055976A1
公开(公告)日：2011-03-03
申请号：US12920847
申请日：2009-03-03
CPC分类号： C12N15/8257 , C07K14/415
摘要： A process of producing a protease in a plant or in plant cells, comprising (a) providing a plant comprising a heterologous nucleotide sequence comprising a coding sequence encoding a fusion protein, said fusion protein comprising: an apoplast or plastid signal peptide; a SUMO protein or a derivative of a SUMO protein; and a zymogen of said protease, and (b) expressing said fusion protein.
摘要翻译： 一种在植物或植物细胞中产生蛋白酶的方法，包括（a）提供包含异源核苷酸序列的植物，所述异源核苷酸序列包含编码融合蛋白的编码序列，所述融合蛋白包含：质外体或质体信号肽; SUMO蛋白或SUMO蛋白的衍生物; 和所述蛋白酶的酶原，和（b）表达所述融合蛋白。
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公开(公告)号：US20100071084A1
公开(公告)日：2010-03-18
申请号：US12438743
申请日：2007-09-06
CPC分类号： C12N15/8203 , C12N15/8216 , C12N2770/26011 , C12N2770/26043
摘要： Nucleic acid comprising or encoding an RNA replica comprising, in this order, the following segments (i) to (iii): i) a nucleic acid sequence encoding a potexvirus RNA-dependent RNA polymerase or a function-conservative variant thereof; ii) a nucleic acid sequence comprising: a) a potexvirus triple gene block or a function-conservative variant thereof and b) a sequence encoding a potexviral coat protein or a function-conservative variant thereof; or a sequence encoding a tobago viral movement protein; and iii) a heterologous nucleic acid sequence expressible from said replica in a plant or in plant tissue.
摘要翻译： 包含或编码RNA复制品的核酸，其依次包括以下片段（i）至（iii）：i）编码病毒RNA依赖性RNA聚合酶或其功能保守变体的核酸序列; ii）核酸序列，其包含：a）罐状病毒三重基因嵌段或其功能保守变体，和b）编码潜在外壳蛋白或其功能保守变体的序列; 或编码多巴胺病毒运动蛋白的序列; 和iii）在植物或植物组织中可从所述复制品表达的异源核酸序列。
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公开(公告)号：US08193410B2
公开(公告)日：2012-06-05
申请号：US12621765
申请日：2009-11-19
申请人： Stefan Werner , Anatoly Giritch , Serik Eliby , Sylvestre Marillonnet , Victor Klimyuk , Yuri Gleba
发明人： Stefan Werner , Anatoly Giritch , Serik Eliby , Sylvestre Marillonnet , Victor Klimyuk , Yuri Gleba
CPC分类号： C12N15/8274 , C12N15/8213 , C12N15/8216 , C12N15/8231 , C12N15/8265 , C12N15/8289
摘要： A process of producing a transgenic multi-cellular plants or parts thereof expressing a trait of interest, said trait having a controlled distribution of said trait to progeny, wherein said process comprises (i) producing a first plant or a cell thereof having in a first locus of a nuclear chromosome a first heterologous nucleotide sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest, (ii) producing a second plant or a cell thereof having in a second locus of a nuclear chromosome homologous to said nuclear chromosome of step (i), a second heterologous nucleotide sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, and (iii) hybridising said first and said second plant or cells thereof to generate progeny exhibiting said functional trait of interest due to binding between a protein or polypeptide encoded by said first heterologous nucleotide sequence and a protein or polypeptide encoded by said second heterologous nucleotide sequence. Further, the invention provides a process of producing hybrid seeds for agriculture.
摘要翻译： 一种生产表达感兴趣性状的转基因多细胞植物或其部分的方法，所述性状具有所述性状受控于后代的分布，其中所述方法包括（i）产生第一种植物或其细胞，其具有第一种 核染色体的位点是包含编码所述目的性状的核苷酸序列的第一片段的第一异源核苷酸序列，（ii）产生第二种植物或细胞，所述第二种植物或细胞在与所述核染色体同源的核染色体的第二位点中具有 步骤（i），第二异源核苷酸序列，其包含编码所述感兴趣性状的核苷酸序列的第二片段，和（iii）将所述第一和第二植物或细胞杂交以产生由于结合而表现出所述目的功能性状的后代 在由所述第一异源核苷酸序列编码的蛋白质或多肽与由所述第二异源核苷酸编码的蛋白质或多肽之间 异源核苷酸序列。 此外，本发明提供了一种生产用于农业的杂种种子的方法。
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公开(公告)号：US08053632B2
公开(公告)日：2011-11-08
申请号：US10535780
申请日：2003-11-20
CPC分类号： C12N15/8216 , C12N15/8217 , C12N15/8237
摘要： A method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly introducing a polypeptide from a cell-free composition into cells containing said heterologous nucleic acid wherein said polypeptide and said heterologous nucleic acid are mutually adapted such that said polypeptide is capable of switching on said cellular process of interest.
摘要翻译： 一种控制遗传修饰植物的方法，包括（a）提供经遗传修饰的植物，其中所述经遗传修饰的植物的细胞含有异源核酸，由此所述遗传修饰的植物在细胞过程中是无活性的 兴趣，（b）通过将多肽从无细胞组合物直接引入到含有所述异源核酸的细胞中，其中所述多肽和所述异源核酸相互适应使得所述多肽能够导通，从而切换所述感兴趣的细胞过程 所述细胞过程是感兴趣的。
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