公开(公告)号：US20230365954A1

公开(公告)日：2023-11-16

申请号：US18201392

申请日：2023-05-24

申请人： TAKEDA PHARMACEUTICAL COMPANY LIMITED

发明人： Brian Dwyer ,  Bohong Zhang ,  Jun Hu ,  Muthuraman Meiyappan ,  Thomas Miller ,  Paul McLean ,  Clark Pan

IPC分类号： C12N9/40 ,  B01J20/286 ,  B01J20/289 ,  B01J20/32

CPC分类号： C12N9/2465 ,  C12Y302/01022 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3253 ,  B01J20/3255

摘要： The invention relates to an affinity resin functionalized with small molecule inhibitors of glycoside-cleaving enzymes, e.g., α-galactosidase A (α-Gal A), glucocerebrosidase (GCB), β-galactosidase, and acid alpha-glucosidase (GAA), and a method for purifying glycoside-cleaving enzymes produced in a cell line using the small molecule inhibitor-functionalized affinity resin.

公开(公告)号：US11685912B2

公开(公告)日：2023-06-27

申请号：US16978417

申请日：2019-03-05

申请人： TAKEDA PHARMACEUTICAL COMPANY LIMITED

发明人： Brian Dwyer ,  Bohong Zhang ,  Jun Hu ,  Muthuraman Meiyappan ,  Thomas Miller ,  Paul McLean ,  Clark Pan

IPC分类号： C12N9/40 ,  B01J20/286 ,  B01J20/289 ,  B01J20/32

CPC分类号： C12N9/2465 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3253 ,  B01J20/3255 ,  C12Y302/01022

摘要： The invention relates to an affinity resin functionalized with small molecule inhibitors of glycoside-cleaving enzymes, e.g., α-galactosidase A (α-Gal A), glucocerebrosidase (GCB), β-galactosidase, and acid alpha-glucosidase (GAA), and a method for purifying glycoside-cleaving enzymes produced in a cell line using the small molecule inhibitor-functionalized affinity resin.