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公开(公告)号：US20100041127A1
公开(公告)日：2010-02-18
申请号：US12581431
申请日：2009-10-19
申请人： TANJA JARHEDE , Per Kjellin , Anita Larsson , Hans Sjöbom
发明人： TANJA JARHEDE , Per Kjellin , Anita Larsson , Hans Sjöbom
CPC分类号： G01N33/548 , B82Y30/00 , G01N2800/52 , Y10S436/809
摘要： A method of preparing a protein-resistant reactive solid support surface is disclosed. The method comprises the steps of providing a solid support having a hydrogel coating with a plurality of binding elements, coupling a protein resistant compound to the hydrogel via a first fraction of the binding elements, and coupling at least one binding agent to the hydrogel via a second fraction of the binding elements, whereby the protein resistant compound and the at least one binding agent are co-immobilized to the hydrogel. Also the use of the reactive surface in analysis, such as immunogenicity assays, is disclosed.
摘要翻译： 公开了一种制备蛋白质抗性活性固体支持物表面的方法。 该方法包括以下步骤：提供具有多个结合元件的水凝胶涂层的固体支持物，通过结合元件的第一部分将蛋白质抗性化合物偶联至水凝胶，以及通过经由 结合元件的第二部分，其中蛋白质抗性化合物和至少一种结合剂共同固定在水凝胶上。 还公开了分析中的反应性表面的使用，例如免疫原性测定法。
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公开(公告)号：US20060094060A1
公开(公告)日：2006-05-04
申请号：US11249038
申请日：2005-10-11
申请人： Tanja Jarhede , Per Kjellin , Anita Larsson , Hans Sjobom
发明人： Tanja Jarhede , Per Kjellin , Anita Larsson , Hans Sjobom
CPC分类号： G01N33/548 , B82Y30/00 , G01N2800/52 , Y10S436/809
摘要： A method of preparing a protein-resistant reactive solid support surface is disclosed. The method comprises the steps of providing a solid support having a hydrogel coating with a plurality of binding elements, coupling a protein resistant compound to the hydrogel via a first fraction of the binding elements, and coupling at least one binding agent to the hydrogel via a second fraction of the binding elements, whereby the protein resistant compound and the at least one binding agent are co-immobilized to the hydrogel. Also the use of the reactive surface in analysis, such as immunogenicity assays, is disclosed.
摘要翻译： 公开了一种制备蛋白质抗性活性固体支持物表面的方法。 该方法包括以下步骤：提供具有多个结合元件的水凝胶涂层的固体支持物，通过结合元件的第一部分将蛋白质抗性化合物偶联至水凝胶，以及通过经由 结合元件的第二部分，其中蛋白质抗性化合物和至少一种结合剂共同固定在水凝胶上。 还公开了分析中的反应性表面的使用，例如免疫原性测定法。
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公开(公告)号：US08093005B2
公开(公告)日：2012-01-10
申请号：US12581431
申请日：2009-10-19
申请人： Tanja Jarhede , Per Kjellin , Anita Larsson , Hans Sjobom
发明人： Tanja Jarhede , Per Kjellin , Anita Larsson , Hans Sjobom
CPC分类号： G01N33/548 , B82Y30/00 , G01N2800/52 , Y10S436/809
摘要： A method of preparing a protein-resistant reactive solid support surface is disclosed. The method comprises the steps of providing a solid support having a hydrogel coating with a plurality of binding elements, coupling a protein resistant compound to the hydrogel via a first fraction of the binding elements, and coupling at least one binding agent to the hydrogel via a second fraction of the binding elements, whereby the protein resistant compound and the at least one binding agent are co-immobilized to the hydrogel. Also the use of the reactive surface in analysis, such as immunogenicity assays, is disclosed.
摘要翻译： 公开了一种制备蛋白质抗性活性固体支持物表面的方法。 该方法包括以下步骤：提供具有多个结合元件的水凝胶涂层的固体支持物，通过结合元件的第一部分将蛋白质抗性化合物偶联至水凝胶，以及通过经由 结合元件的第二部分，其中蛋白质抗性化合物和至少一种结合剂共同固定在水凝胶上。 还公开了分析中的反应性表面的使用，例如免疫原性测定法。
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公开(公告)号：US08263415B2
公开(公告)日：2012-09-11
申请号：US12377021
申请日：2007-09-12
申请人： Henrik Berling , Tanja Jarhede , Anita Larsson , Hakan Roos
发明人： Henrik Berling , Tanja Jarhede , Anita Larsson , Hakan Roos
IPC分类号： G01N33/566
CPC分类号： G01N33/54306 , G01N15/1459 , G01N15/1484 , G01N33/54366 , G01N33/54393
摘要： A method of determining the total concentration of an analyte in a fluid sample, wherein at least part of the analyte is present as a complex with an analyte-binding species. The methods includes the steps of: a) subjecting the sample to conditions that reduce the binding affinity between analyte and analyte-binding species sufficiently to dissociate substantially any analyte complex and provide substantially all analyte in free form, b) subjecting the sample to conditions that restore the binding affinity between analyte and analyte-binding species, and c) immediately after the binding affinity has been restored, and before any substantial re-complexing of the analyte has taken place, determining the concentration of free analyte in the sample. A method of determining the concentration of complex-bound analyte in a sample is also disclosed.
摘要翻译： 确定流体样品中分析物的总浓度的方法，其中至少部分分析物与分析物结合物质作为复合物存在。 所述方法包括以下步骤：a）使样品经受降低分析物和分析物结合物质之间的结合亲和力的条件，足以使基本上解析任何分析物复合物并基本上提供游离形式的所有分析物，b）使样品经受 恢复分析物和分析物结合物质之间的结合亲和力，以及c）在结合亲和力恢复后立即，并且在分析物发生任何显着的重新络合之前，确定样品中游离分析物的浓度。 还公开了测定样品中复合物结合分析物浓度的方法。
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公开(公告)号：US20100167422A1
公开(公告)日：2010-07-01
申请号：US12377021
申请日：2007-09-12
申请人： Henrik Berling , Tanja Jarhede , Anita Larsson , Hakan Roos
发明人： Henrik Berling , Tanja Jarhede , Anita Larsson , Hakan Roos
IPC分类号： G01N33/543
CPC分类号： G01N33/54306 , G01N15/1459 , G01N15/1484 , G01N33/54366 , G01N33/54393
摘要： A method of determining the total concentration of an analyte in a fluid sample, wherein at least part of the analyte is present as a complex with an analyte-binding species. The methods includes the steps of: a) subjecting the sample to conditions that reduce the binding affinity between analyte and analyte-binding species sufficiently to dissociate substantially any analyte complex and provide substantially all analyte in free form, b) subjecting the sample to conditions that restore the binding affinity between analyte and analyte-binding species, and c) immediately after the binding affinity has been restored, and before any substantial re-complexing of the analyte has taken place, determining the concentration of free analyte in the sample. A method of determining the concentration of complex-bound analyte in a sample is also disclosed.
摘要翻译： 确定流体样品中分析物的总浓度的方法，其中至少部分分析物与分析物结合物质作为复合物存在。 所述方法包括以下步骤：a）使样品经受降低分析物和分析物结合物质之间的结合亲和力的条件，足以使基本上解析任何分析物复合物并基本上提供游离形式的所有分析物，b）使样品经受 恢复分析物和分析物结合物质之间的结合亲和力，以及c）在结合亲和力恢复后立即，并且在分析物发生任何显着的重新络合之前，确定样品中游离分析物的浓度。 还公开了测定样品中复合物结合分析物浓度的方法。
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公开(公告)号：US06207381B1
公开(公告)日：2001-03-27
申请号：US09165700
申请日：1998-10-02
申请人： Anita Larsson , Björn Persson
发明人： Anita Larsson , Björn Persson
IPC分类号： C12Q168
CPC分类号： C12Q1/6813 , C12Q1/6851 , C12Q1/689 , C12Q2600/156 , Y10S435/81
摘要： A method of determining the binding of an oligonucleotide probe to a test nucleic acid sequence comprises the steps of: (a) providing the test nucleic acid sequence in single-stranded form, (b) contacting the test nucleic acid sequence under hybridizing conditions with a solution containing an oligonucleotide probe which is complementary to a defined portion of a standard nucleic acid sequence, (c) immobilizing to a solid support a nucleic acid fragment at least part of which is complementary to said oligonucleotide probe, (d) contacting the solution from step (b) with said solid support, and (e) determining the amount of binding of oligonucleotide probe present in said solution to its complementary nucleic acid fragment on the second solid support, said amount being inversely related to the amount of binding of the oligonucleotide probe to the test nucleic acid sequence.
摘要翻译： 确定寡核苷酸探针与测试核酸序列的结合的方法包括以下步骤：（a）以单链形式提供测试核酸序列，（b）使测试核酸序列在杂交条件下与 包含与标准核酸序列的限定部分互补的寡核苷酸探针的溶液，（c）将至少一部分与所述寡核苷酸探针互补的核酸片段固定在固体支持物上，（d）使来自 步骤（b）与所述固体支持物，和（e）确定存在于所述溶液中的寡核苷酸探针与其在第二固体支持物上的互补核酸片段的结合量，所述量与寡核苷酸的结合量成反比 探测测试核酸序列。
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7.发明授权Characterizing macromolecules interacting with at least three ligands on a sensor 失效表征与传感器上至少三个配体相互作用的大分子
公开(公告)号：US5554541A
公开(公告)日：1996-09-10
申请号：US366443
申请日：1994-12-30
IPC分类号： G01N21/55 , G01N33/543 , G01N33/68
CPC分类号： G01N33/6878 , G01N33/54373 , G01N21/211 , G01N21/553 , Y10S436/805
摘要： A method of characterizing a macromolecule by studying its interactions with ligands comprises the determination of the mutual influence of ligand interactions by, after the macromolecule has interacted with at least one ligand, contacting the macromolecule with at least one additional ligand, either the macromolecule or the additional ligand or ligands having been bound to a sensor surface, determining interaction by detecting a consequential change in the physico-chemical properties of the sensor surface, and on the basis of the determined mutual dependence between the ligand interactions discriminating between epitopes of the macromolecule and mapping their relative positions.
摘要翻译： 通过研究其与配体的相互作用来表征大分子的方法包括测定配体相互作用的相互影响，在大分子与至少一种配体相互作用后，将大分子与至少一种另外的配体，大分子或 已经结合到传感器表面的另外的配体或配体，通过检测传感器表面的物理化学性质的相应变化来确定相互作用，并且基于所确定的配位体相互作用之间的相互依赖性，所述相互作用区分大分子的表位和 映射他们的相对位置。
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公开(公告)号：US5656504A
公开(公告)日：1997-08-12
申请号：US424368
申请日：1995-04-25
申请人： Viveca Johansson , Anita Larsson , Inger Ronnberg
发明人： Viveca Johansson , Anita Larsson , Inger Ronnberg
IPC分类号： G01N33/543
CPC分类号： G01N33/54393 , Y10S435/962 , Y10S435/97 , Y10S436/825
摘要： In assays of the type which comprise contacting the sample containing the analyte to be detected with a solid phase surface supporting a ligand capable of binding the analyte, undesired binding to the surface is prevented to a substantial degree by adding to the sample one or more components of the material forming the ligand supporting solid phase surface, which components are in at least partially soluble form and capable of interacting with constituents of the sample medium.
摘要翻译： PCT No.PCT / SE93 / 00874 Sec。 371日期1995年04月25日 102（e）日期1995年4月25日PCT提交1992年10月26日PCT公布。 公开号WO94 / 10573 日期1994年5月11日在包括将待检测分析物的样品与支持能够结合分析物的配体的固相表面接触的类型的测定中，通过向样品中添加不期望的与表面的结合被大大程度地防止 形成支持固相表面的配体的材料的一种或多种组分，该组分至少部分可溶形式并且能够与样品介质的组分相互作用。
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公开(公告)号：US20110070574A1
公开(公告)日：2011-03-24
申请号：US12994189
申请日：2009-06-01
CPC分类号： G01N21/658 , G01N21/553 , G01N33/54306 , G01N33/54373
摘要： A method of determining the concentration of a virus or antigen thereof in a sample comprises the steps of: providing a sensor surface having immobilized thereto a virus antigen or a virus antigen analogue, mixing the sample with a known amount of antibody to the virus antigen to obtain a predetermined concentration of antibody to the antigen in the sample mixture, contacting the sample mixture with the sensor surface to bind free antibody in the mixture to the sensor surface, measuring the response of the sensor surface to the binding of free antibody, and determining the concentration of the virus or antigen in the sample from a calibration curve prepared by measuring the responses obtained for mixtures containing the predetermined concentration of antibody and different concentrations of virus.
摘要翻译： 确定样品中病毒或其抗原浓度的方法包括以下步骤：提供固定有病毒抗原或病毒抗原类似物的传感器表面，将样品与已知量的抗体混合到病毒抗原上 在样品混合物中获得与抗原的预定浓度的抗体，使样品混合物与传感器表面接触，将混合物中的游离抗体结合到传感器表面，测量传感器表面对游离抗体结合的响应，并确定 来自通过测量获得的含有预定浓度的抗体和不同浓度的病毒的混合物的反应制备的校准曲线的样品中病毒或抗原的浓度。
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