公开(公告)号：US20170227444A1

公开(公告)日：2017-08-10

申请号：US15263419

申请日：2016-09-13

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Bahram Jalai ,  Eric Diebold ,  Brandon Buckley

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/6486 ,  G01N33/537 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2021/6421 ,  G01N2201/067

Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.