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公开(公告)号：US11280718B2
公开(公告)日：2022-03-22
申请号：US17068573
申请日：2020-10-12
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Bahram Jalali , Eric D. Diebold , Brandon Buckley
IPC: G01N15/14 , G01N33/537 , G01N21/64 , G01N15/10
Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.
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2.发明授权公开(公告)号：US10408758B2
公开(公告)日：2019-09-10
申请号：US15961678
申请日：2018-04-24
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Eric D. Diebold , Bahram Jalali , Brandon Buckley
Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.
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3.发明申请公开(公告)号：US20170138857A1
公开(公告)日：2017-05-18
申请号：US15221065
申请日：2016-07-27
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Eric D. Diebold , Bahram Jalali , Brandon Buckley
CPC classification number: G01N21/6408 , G01N21/05 , G01N21/6458 , G01N21/6486 , G01N2021/6415 , G01N2021/6419 , G01N2201/06113 , G02B21/0076 , G02B21/0084 , G02B21/16 , G02B27/1006 , G02F1/11
Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.
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4.发明申请APPARATUS AND METHODS FOR FLUORESCENCE IMAGING USING RADIOFREQUENCY-MULTIPLEXED EXCITATION 有权使用放射性多重激发进行荧光成像的装置和方法公开(公告)号：US20160003741A1
公开(公告)日：2016-01-07
申请号：US14792282
申请日：2015-07-06
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Eric D. Diebold , Bahram Jalali , Brandon Buckley
CPC classification number: G01N21/6408 , G01N21/05 , G01N21/6458 , G01N21/6486 , G01N2021/6415 , G01N2021/6419 , G01N2201/06113 , G02B21/0076 , G02B21/0084 , G02B21/16 , G02B27/1006 , G02F1/11
Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.
Abstract translation: 使用射频复用激发进行荧光成像的装置和方法。 一个装置将激发激光束分成Mach-Zehnder干涉仪的两个臂。 第一光束中的光被声光偏转器偏移，声光偏转器由相位设计的射频梳驱动，旨在最小化峰值与平均功率比。 该RF梳产生具有一定范围的输出角和频移的多个偏转光束。 第二个波束使用声光移频器在频率上移动。 在第二分光器组合之后，使用传统的激光扫描显微镜透镜系统将两个光束聚焦到样品上的线上。 声光偏转器对整行像素的同时激励进行频率编码，这使得能够使用单个光电倍增管和数字相位相干信号恢复技术来检测和解复用荧光图像。
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公开(公告)号：US20230280259A1
公开(公告)日：2023-09-07
申请号：US18117708
申请日：2023-03-06
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Bahram Jalali , Eric D. Diebold , Brandon Buckley
IPC: G01N15/14 , G01N33/537 , G01N21/64
CPC classification number: G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N21/64 , G01N33/537 , G01N2015/1006
Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.
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Patent Agency Ranking
公开(公告)号：US11630053B2
公开(公告)日：2023-04-18
申请号：US17666841
申请日：2022-02-08
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Bahram Jalali , Eric D. Diebold , Brandon Buckley
IPC: G01N15/14 , G01N33/537 , G01N21/64 , G01N15/10
Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.
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7.发明申请公开(公告)号：US20230052995A1
公开(公告)日：2023-02-16
申请号：US17745206
申请日：2022-05-16
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Eric D. Diebold , Bahram Jalali , Brandon Buckley
Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.
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8.发明授权公开(公告)号：US11371937B2
公开(公告)日：2022-06-28
申请号：US16528463
申请日：2019-07-31
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Eric D. Diebold , Bahram Jalali , Brandon Buckley
Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.
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9.发明授权公开(公告)号：US11327016B2
公开(公告)日：2022-05-10
申请号：US16528436
申请日：2019-07-31
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Eric D. Diebold , Bahram Jalali , Brandon Buckley
Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.
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公开(公告)号：US20170350803A1
公开(公告)日：2017-12-07
申请号：US15672051
申请日：2017-08-08
Applicant: The Regents of the University of California
Inventor： Bahram Jalali , Eric Diebold , Brandon Buckley
CPC classification number: G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N21/64 , G01N21/6428 , G01N21/6458 , G01N21/6486 , G01N33/537 , G01N2015/1006 , G01N2015/1477 , G01N2021/6421 , G01N2201/067
Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.
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