公开(公告)号：US20210268465A1

公开(公告)日：2021-09-02

申请号：US17268954

申请日：2019-08-16

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Joseph de Rutte ,  Robert Dimatteo

IPC: B01J13/00 ,  C09B67/02 ,  C09D11/037 ,  C09D11/023 ,  H01F1/44

Abstract: Systems and methods are described herein that create discrete volumes associated with solid-phase particles (e.g., drop-carrier particles) suspended in an immiscible phase (e.g., dropicles). One embodiment of the system includes a plurality of hydrogel-based drop-carrier particles containing a microscale voids or cavities that hold an aqueous phase droplet of fluid within each drop-carrier particle. The plurality of hydrogel drop-carrier particles associated with aqueous drops are suspended as individual elements in an immiscible oil phase. The microscale hydrogel drop-carrier particles containing the voids or cavities may be manufactured using microfluidic droplet generators. The dropicles may be used to analyze single-entities (e.g., single-molecules and single-cells) and analytes.

公开(公告)号：US20250128229A1

公开(公告)日：2025-04-24

申请号：US18959449

申请日：2024-11-25

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Joseph de Rutte ,  Robert Dimatteo

IPC: B01J13/00 ,  C08J3/12 ,  C08J3/24 ,  C09B67/02 ,  H01F1/44

Abstract: Systems and methods are described herein that create discrete volumes associated with solid-phase particles (e.g., drop-carrier particles) suspended in an immiscible phase (e.g., dropicles). One embodiment of the system includes a plurality of hydrogel-based drop-carrier particles containing a microscale voids or cavities that hold an aqueous phase droplet of fluid within each drop-carrier particle. The plurality of hydrogel drop-carrier particles associated with aqueous drops are suspended as individual elements in an immiscible oil phase. The microscale hydrogel drop-carrier particles containing the voids or cavities may be manufactured using microfluidic droplet generators. The dropicles may be used to analyze single-entities (e.g., single-molecules and single-cells) and analytes.

公开(公告)号：US20240302264A1

公开(公告)日：2024-09-12

申请号：US18560106

申请日：2022-05-05

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Joseph de Rutte ,  Robert Dimatteo

IPC: G01N15/149 ,  G01N15/14

CPC classification number: G01N15/149 ,  G01N15/1459

Abstract: A method of analyzing shaped particles using a flow cytometer or a fluorescence activated cell sorter (FACS) includes flowing a population of shaped particles with at least some of the population of shaped particles having cells loaded therein through the flow cytometer or FACS and optically interrogating the shaped particles in the flow cytometer or FACS to measure scattered light for each shaped particle. A target shaped particle having a cell loaded therein is detected based at least in part on a measurement of forward scattered light, side scattered light, or back scattered light. The target shaped particle may also be identified with a measured fluorescence signal level. Sorting of target shaped particles may be optimized by adjusting one or more of a drop delay or a sorting mask configuration for the flow cytometer or FACS.