-
公开(公告)号：US12233407B2
公开(公告)日：2025-02-25
申请号：US18161322
申请日：2023-01-30
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Chueh-Yu Wu
IPC: B01L3/02 , B01L3/00 , C12N1/04 , C12N5/071 , C12N5/0783 , C12Q1/6806 , G01N33/543
Abstract: Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and/or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and/or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.
-
公开(公告)号：US20240416008A1
公开(公告)日：2024-12-19
申请号：US18443314
申请日：2024-02-16
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Westbrook Weaver , Tatiana Segura , Jaekyung Koh , Philip Scumpia , Donald R. Griffin
Abstract: A hydrogel material for use in a human subject or other mammal includes a collection of microgel particles having one or more network cross linker components, wherein the microgel particles, when exposed to an endogenous or exogenous annealing agent, links the microgel particles together in situ to form a covalently-stabilized scaffold of microgel particles having pores formed between the microgel particles wherein the pores are substantially devoid of hydrogel.
-
公开(公告)号：US20230257732A1
公开(公告)日：2023-08-17
申请号：US18006382
申请日：2021-08-04
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Mark van Zee , Dino Di Carlo , Joseph de Rutte , Sohyung Lee , Robert Damoiseaux , Cayden Williamson
CPC classification number: C12N11/04 , C12N5/0012 , C12N5/00 , C12N1/12 , C12N11/10 , C12N2537/10 , C12N2539/10 , C12N2509/00 , C12N2533/50 , C12N2533/70
Abstract: Degradable hollow shell particles are disclosed that can encapsulate cells within the hollow inner cavity that allows for the high-throughput screening and sorting of the encapsulated cells based on their phenotypic properties. The solid-phase of the particle is porous such that solution exchange can occur between the external environment and the interior cavity. Further, the solid-phase contains degradable crosslinkers and can be degraded to release enclosed biological entities. An example embodiment consists of encapsulating a cell within the hollow shell particle, allowing the cell to accumulate biomass, selecting hollow shell particles based on accumulated biomass, and degrading the hollow shell particles to release the cells and develop hyper-producing cell lines. Exemplary cell types include microalgae, mammalian cells, bacteria, yeast, and fungi.
-
公开(公告)号：US11596909B2
公开(公告)日：2023-03-07
申请号：US16888563
申请日：2020-05-29
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Soroush Kahkeshani
IPC: B01F23/411 , A61K9/00 , B01L3/00 , B01F33/30 , B01F33/3032 , A61M37/00 , G01N35/10
Abstract: A microfluidic device for forming droplets includes at least one ferrofluid reservoir disposed in the microfluidic device and containing a ferrofluid therein. The microfluidic device includes a continuous-phase reservoir disposed in the microfluidic device and containing an oil phase therein and one or more microfluidic channels connecting between the at least one ferrofluid reservoir and the continuous-phase reservoir, the continuous-phase reservoir comprising a step region having an increased height as compared to a height of the one or more microfluidic channels. To form droplets an externally applied magnetic field is applied to the device to pull the ferrofluid into the continuous-phase reservoir, whereby droplets are formed at step region.
-
公开(公告)号：US20220233413A1
公开(公告)日：2022-07-28
申请号：US17609384
申请日：2020-05-05
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Chueh-Yu Wu , Andrea L. Bertozzi , Bao Wang , Joseph de Rutte , Kyung HA
Abstract: An emulsion system includes a plurality of monodisperse particle-drops. Each particle-drop is formed by a single elongated drop-carrier particle disposed in an oil-based continuous phase, wherein the single elongated drop-carrier particle comprises an elongate body with an opening at one end thereof. The single elongated drop-carrier particle has a hydrophilic interior region containing an aqueous droplet and a hydrophobic exterior region. The aqueous droplet and/or a surface of the hydrophilic interior region may contain one or more reagents, analytes, labels, reporter molecules, and/or cells.
-
Patent Agency Ranking
公开(公告)号：US20210354121A1
公开(公告)日：2021-11-18
申请号：US17387990
申请日：2021-07-28
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Chueh-Yu Wu , Ghulam Destgeer , Mengxing Ouyang
IPC: B01L3/02 , B01L3/00 , G01N33/543
Abstract: Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and/or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and/or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.
-
公开(公告)号：US11169078B2
公开(公告)日：2021-11-09
申请号：US15868025
申请日：2018-01-11
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Daniel R. Gossett , Henry T. K. Tse
IPC: G01N15/14 , G16B99/00 , G01N33/574 , C12Q1/02 , B01L3/00 , G01N33/487 , G01N15/00 , G01N15/10
Abstract: A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.
-
公开(公告)号：US20210268465A1
公开(公告)日：2021-09-02
申请号：US17268954
申请日：2019-08-16
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Joseph de Rutte , Robert Dimatteo
IPC: B01J13/00 , C09B67/02 , C09D11/037 , C09D11/023 , H01F1/44
Abstract: Systems and methods are described herein that create discrete volumes associated with solid-phase particles (e.g., drop-carrier particles) suspended in an immiscible phase (e.g., dropicles). One embodiment of the system includes a plurality of hydrogel-based drop-carrier particles containing a microscale voids or cavities that hold an aqueous phase droplet of fluid within each drop-carrier particle. The plurality of hydrogel drop-carrier particles associated with aqueous drops are suspended as individual elements in an immiscible oil phase. The microscale hydrogel drop-carrier particles containing the voids or cavities may be manufactured using microfluidic droplet generators. The dropicles may be used to analyze single-entities (e.g., single-molecules and single-cells) and analytes.
-
9.发明申请公开(公告)号：US20210018491A1
公开(公告)日：2021-01-21
申请号：US17033488
申请日：2020-09-25
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Peter Tseng , Ivan Pushkarsky
Abstract: A system for assaying forces applied by cells includes an optically transparent substrate comprising a soft material having a Young's modulus within the range of about 3 kPa to about 100 kPa. An array of molecular patterns is disposed on a surface of the optically transparent substrate, the molecular patterns include fluorophore-conjugated patterns adherent to cells. The system includes at least one light source configured to excite the fluorophore-conjugated patterns and an imaging device configured to capture fluorescent light emitted from the fluorophore-conjugated patterns. Dimensional changes in the size of the patterns are used to determine contractile forces imparted by cells located on the patterns.
-
公开(公告)号：US10688453B2
公开(公告)日：2020-06-23
申请号：US15767979
申请日：2016-10-07
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Soroush Kahkeshani
Abstract: A microfluidic device for forming droplets includes at least one ferrofluid reservoir disposed in the microfluidic device and containing a ferrofluid therein. The microfluidic device includes a continuous-phase reservoir disposed in the microfluidic device and containing an oil phase therein and one or more microfluidic channels connecting between the at least one ferrofluid reservoir and the continuous-phase reservoir, the continuous-phase reservoir comprising a step region having an increased height as compared to a height of the one or more microfluidic channels. To form droplets an externally applied magnetic field is applied to the device to pull the ferrofluid into the continuous-phase reservoir, whereby droplets are formed at step region.
-
-
-
-
-
-
-
-
-
Search Results
108
records
(took 0.032 seconds)
