公开(公告)号：US20240199512A1

公开(公告)日：2024-06-20

申请号：US18393900

申请日：2023-12-22

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  NATIONAL TECHNOLOGY & ENGINEERING SOLUTIONS OF SANDIA, LLC

发明人： Ezinne ACHINIVU ,  Mood MOHAN ,  Hemant CHOUDHARY ,  Lalitendu DAS ,  Venkataramana R. PIDATALA ,  Harsha D. MAGURUDENIYA ,  Kaixuan HUANG ,  John M. GLADDEN ,  Blake A. SIMMONS

IPC分类号： C07C7/08

CPC分类号： C07C7/08

摘要： The present invention provides for a method to deconstruct a biomass: the method comprising: (a) introducing a solvent comprising a polyamine, or a mixture of polyamines, to a biomass to dissolve at least part of solid biomass in the solvent, wherein the polyamine is a Brønsted or Lewis base, and/or the polyamine is a hydrogen bond donor and/or acceptor; (b) optionally introducing an enzyme and/or a microbe to the solubilized biomass mixture such that the enzyme and/or microbe produces a sugar from the solubilized biomass mixture; (c) optionally separating the sugar from the solubilized biomass mixture; and (d) optionally separating the lignan from the solubilized biomass mixture.

公开(公告)号：US20240318189A1

公开(公告)日：2024-09-26

申请号：US18545629

申请日：2023-12-19

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  NATIONAL TECHNOLOGY & ENGINEERING SOLUTIONS OF SANDIA, LLC

发明人： Gina Marie GEISELMAN ,  James KIRBY ,  Taek Soon LEE ,  John M. GLADDEN ,  Blake A. SIMMONS

IPC分类号： C12N15/81 ,  C10L1/04 ,  C12N1/16 ,  C12N9/88 ,  C12P5/00

CPC分类号： C12N15/815 ,  C10L1/04 ,  C12N1/16 ,  C12N9/88 ,  C12P5/007 ,  C12Y402/03037 ,  C10L2200/043 ,  C10L2270/04 ,  C10L2290/26

摘要： The present invention provides for a genetically modified fungal host cell capable of producing prespatane and/or epi-isozizaene comprising prespatane synthase (PPS) and/or epi-isozizaene synthase (EIZS).

公开(公告)号：US20240102057A1

公开(公告)日：2024-03-28

申请号：US18466660

申请日：2023-09-13

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  NATIONAL TECHNOLOGY & ENGINEERING SOLUTIONS OF SANDIA, LLC

发明人： Alberto RODRIGUEZ ,  Jamie A. MEADOWS ,  Ning SUN ,  Blake A. SIMMONS ,  John M. GLADDEN

IPC分类号： C12P7/22 ,  C12N9/18

CPC分类号： C12P7/22 ,  C12N9/18 ,  C12Y301/01093

摘要： The present invention provides for a method for producing a 4-vinylphenol (4VP) and/or 4-vinylguaiacol (4VG), the method comprising: (a) providing a host cell capable of expressing a polypeptide having a phenolic acid decarboxylase (PAD) enzymatic activity wherein the polypeptide is capable of converting p-coumaric (CA) and/or ferulic acid (FA) into 4-vinylphenol (4VP) and/or 4-vinylguaiacol (4VG), respectively; and (b) culturing the host cell in a culture medium to express the polypeptide such that the polypeptide converts CA and/or FA into 4VP and/or 4VG, respectively; wherein the culture medium comprises an organic overlay or phase.

公开(公告)号：US20230078811A1

公开(公告)日：2023-03-16

申请号：US17800871

申请日：2021-02-18

申请人： The Regents of the University of California ,  National Technology & Engineering Solutions of Sandia, LLC

发明人： Ezinne C. ACHINIVU ,  John M. GLADDEN ,  Hemant CHOUDHARY ,  Blake A. SIMMONS

IPC分类号： C12P19/02 ,  C12P19/14

摘要： The present invention provides for a method to produce a sugar from a biomass, the method comprising: (a) providing a first mixture comprising a solubilized biomass and a distillable acid-base conjugate salt (DABCS) or deep eutectic solvent (DES), wherein (i) the DABCS is a protic ionic liquid (PIL) or a protic salt comprising a DABCS cation and a DABCS anion, and (ii) the DES is any combination of Lewis or Brønsted acid and base comprising any anionic and/or cationic species that have sufficient vapor pressure so that it can be readily distilled; and (b) distilling at least part of the DABCS from the first mixture in order to separate the at least part of the DABCS from the first mixture.

公开(公告)号：US20230220121A1

公开(公告)日：2023-07-13

申请号：US17925266

申请日：2021-04-29

申请人： National Technology and Engineering Solutions of Sandia, LLC ,  The Regents of the University of California

发明人： Ezinne ACHINIVU ,  John M. GLADDEN ,  Blake A. SIMMONS

IPC分类号： C08B37/00 ,  C12P19/14

CPC分类号： C08B37/0003 ,  C12P19/14

摘要： The present invention provides for a method to produce a sugar compound from a biomass, the method comprising: (a) providing a first mixture comprising a solubilized biomass and an alkanolamine; (b) recovering at least part of the alkanolamine from the first mixture in order to separate the at least part of the alkanolamine from the first mixture; (c) optionally introducing an enzyme and/or a microbe to the first mixture such that the enzyme and/or microbe produce a sugar from the solubilized biomass; and, (d) optionally the sugar is separated from the first mixture.