公开(公告)号：US11649434B2

公开(公告)日：2023-05-16

申请号：US16678521

申请日：2019-11-08

申请人： The Regents of the University of Michigan

发明人： Jason Spence ,  Alyssa Miller

IPC分类号： C12N5/071

CPC分类号： C12N5/0625 ,  C12N5/0688 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/27 ,  C12N2506/45

摘要： The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or cells through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting functional basal-like cells from pluripotent stem cell-derived lung bud tip progenitor organoid tissue through activation of SMAD signaling via activation of TGFβ1 (and/or the TGFβ signaling pathway) and BMP4 (and/or the BMP signaling pathway).

公开(公告)号：US11857697B2

公开(公告)日：2024-01-02

申请号：US15994749

申请日：2018-05-31

申请人： THE REGENTS OF THE UNIVERSITY OF MICHIGAN

发明人： Jason Spence ,  Alyssa Miller

IPC分类号： C12N5/071 ,  C12N5/00 ,  A61K35/42 ,  A61L27/38 ,  C12N5/074 ,  C12N5/0735

CPC分类号： A61L27/3895 ,  A61K35/42 ,  A61L27/3804 ,  A61L27/3813 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3869 ,  C12N5/0018 ,  C12N5/0062 ,  C12N5/0606 ,  C12N5/0607 ,  C12N5/0688 ,  C12N5/0689 ,  A61L2430/22 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/27 ,  C12N2506/45 ,  C12N2513/00

摘要： The invention disclosed herein generally relates to methods and systems for growing, expanding and/or obtaining 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity. In particular, the invention disclosed herein relates to methods and systems for growing human cells having SOX9 protein activity and SOX2+ protein activity in vitro, and for promoting pluripotent stem cell derived ventral-anterior foregut spheroid tissue into 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity.

公开(公告)号：US20180344901A1

公开(公告)日：2018-12-06

申请号：US15994749

申请日：2018-05-31

申请人： THE REGENTS OF THE UNIVERSITY OF MICHIGAN

发明人： Jason Spence ,  Alyssa Miller

IPC分类号： A61L27/38 ,  C12N5/071 ,  C12N5/074 ,  C12N5/0735 ,  C12N5/00

摘要： The invention disclosed herein generally relates to methods and systems for growing, expanding and/or obtaining 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity. In particular, the invention disclosed herein relates to methods and systems for growing human cells having SOX9 protein activity and SOX2+ protein activity in vitro, and for promoting pluripotent stem cell derived ventral-anterior foregut spheroid tissue into 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity.

公开(公告)号：US20230279348A1

公开(公告)日：2023-09-07

申请号：US18138313

申请日：2023-04-24

申请人： The Regents of the University of Michigan

发明人： Jason Spence ,  Alyssa Miller

IPC分类号： C12N5/071

CPC分类号： C12N5/0625 ,  C12N5/0688 ,  C12N2501/999 ,  C12N2506/27 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45

摘要： The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or cells through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting functional basal-like cells from pluripotent stem cell-derived lung bud tip progenitor organoid tissue through activation of SMAD signaling via activation of TGFβ1 (and/or the TGFβ signaling pathway) and BMP4 (and/or the BMP signaling pathway).

公开(公告)号：US20200149004A1

公开(公告)日：2020-05-14

申请号：US16678521

申请日：2019-11-08

申请人： The Regents of the University of Michigan

发明人： Jason Spence ,  Alyssa Miller

IPC分类号： C12N5/071

摘要： The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or cells through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting functional basal-like cells from pluripotent stem cell-derived lung bud tip progenitor organoid tissue through activation of SMAD signaling via activation of TGFβ1 (and/or the TGFβsignaling pathway) and BMP4 (and/or the BMP signaling pathway).