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    Specific detection of deoxyribonucleic acid sequences using novel CRISPR enzyme-mediated detection strategies
    7.
    发明授权
    Specific detection of deoxyribonucleic acid sequences using novel CRISPR enzyme-mediated detection strategies 有权

    公开(公告)号：US11608519B2

    公开(公告)日：2023-03-21

    申请号：US16049460

    申请日：2018-07-30

    申请人： TOKITAE LLC

    发明人： Ted A. Baughman ,  Damian Madan ,  Eric Nalefski ,  Anne-Laure M. Le Ny

    IPC分类号： C12Q1/6832 ,  C12N9/22 ,  C12N15/11

    摘要： Embodiments disclosed herein include devices, methods, and systems for direct, selective, and sensitive detection of single-stranded and double-stranded target DNA sequences from various sources using a Cas12a protein. When activated by binding a target DNA sequence, the Cas12a cleaves a tether releasing a reporter molecule that may then be detected. In some embodiments, the systems, methods, and devices may include a filter or membrane that may help to separate the tethered and untethered reporter molecules. These devices, systems, and techniques allow a user to rapidly process samples that may contain the target DNA, without needing to amplify the target sequences. These devices and methods may be used to assay a wide variety of samples and target DNA sources, for the presence or absence of target DNA sequences. Compositions and kits, useful in practicing these methods, for example detecting a target DNA in a biological sample, are also described.

    SOLUTION-PHASE, TRANS-ACTIVATED REPORTER SYSTEMS FOR USE IN CRISPR-BASED NUCLEIC ACID SEQUENCE DETECTIONS
    8.
    发明申请
    SOLUTION-PHASE, TRANS-ACTIVATED REPORTER SYSTEMS FOR USE IN CRISPR-BASED NUCLEIC ACID SEQUENCE DETECTIONS 有权

    公开(公告)号：US20220106647A1

    公开(公告)日：2022-04-07

    申请号：US17430235

    申请日：2020-02-07

    申请人： Tokitae LLC

    发明人： Ted Baughman ,  Anne-Laure M. Le Ny ,  Philip Leung ,  Damian Madan ,  Eric Nalefski

    IPC分类号： C12Q1/6897 ,  C12N9/22 ,  C12N15/11

    摘要： Embodiments disclosed herein include devices, methods, and systems for direct, selective, and sensitive detection of single-stranded and double-stranded target nucleic acid sequences from various sources in a solution-based system. When activated by binding a target nucleic acid sequence, the Cas protein cleaves a tether separating a reporter molecule from a capture moiety. The capture moiety can then be used to remove, localize, or sequester uncleaved molecule containing intact tethers. In some embodiments, the systems, methods, and devices may include a filter, a membrane, or other molecules that may help to separate the tethered and untethered reporter molecules and/or capture the reporter molecules. These devices, systems, and techniques allow a user to rapidly process samples that may contain the target nucleic acid, in some cases, without needing to amplify the target sequences, and without the need for sophisticated or expensive laboratory equipment. These devices and methods may be used to assay a wide variety of samples and target nucleic acid sources, for the presence or absence of a specific target sequences. Compositions and kits, useful in practicing these methods, for example detecting a target RNA or DNA in a biological sample, are also described.

    SPECIFIC DETECTION OF DEOXYRIBONUCLEIC ACID SEQUENCES USING NOVEL CRISPR ENZYME-MEDIATED DETECTION STRATEGIES
    9.
    发明申请
    SPECIFIC DETECTION OF DEOXYRIBONUCLEIC ACID SEQUENCES USING NOVEL CRISPR ENZYME-MEDIATED DETECTION STRATEGIES 审中-公开

    公开(公告)号：US20200032325A1

    公开(公告)日：2020-01-30

    申请号：US16049460

    申请日：2018-07-30

    申请人： TOKITAE LLC

    发明人： Ted A. Baughman ,  Damian Madan ,  Eric Nalefski ,  Anne-Laure M. Le Ny

    IPC分类号： C12Q1/6832 ,  C12N9/22 ,  C12N15/11

    摘要： Embodiments disclosed herein include devices, methods, and systems for direct, selective, and sensitive detection of single-stranded and double-stranded target DNA sequences from various sources using a programmed Cas12a protein. When activated by binding a target DNA sequence, the Cas12a cleaves a tether releasing a reporter molecule that may then be detected. In some embodiments, the systems, methods, and devices may include a filter or membrane that may help to separate the tethered and untethered reporter molecules. These devices, systems, and techniques allow a user to rapidly process samples that may contain the target DNA, without needing to amplify the target sequences. These devices and methods may be used to assay a wide variety of samples and target DNA sources, for the presence or absence of a specific target DNA sequences. Compositions and kits, useful in practicing these methods, for example detecting a target DNA in a biological sample, are also described.