公开(公告)号：US11975069B2

公开(公告)日：2024-05-07

申请号：US17143871

申请日：2021-01-07

申请人： Massachusetts Institute of Technology ,  Tokitae LLC

发明人： Ana Jaklenec ,  William Gates ,  Philip A. Welkhoff ,  Boris Nikolic ,  Lowell L. Wood, Jr. ,  Robert S. Langer ,  Thanh Duc Nguyen ,  Stephany Yi Tzeng ,  James J. Norman ,  Kevin McHugh

IPC分类号： A61K39/39 ,  A61K39/00 ,  A61K39/12

CPC分类号： A61K39/39 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/545 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/6093 ,  C12N2770/32634 ,  Y02A50/30

摘要： Emulsion-based and micromolded (“MM”) or three dimensional printed (“3DP”) polymeric formulations for single injection of antigen, preferably releasing at two or more time periods, have been developed. Formulations are preferably formed of biocompatible, biodegradable polymers. Discrete regions encapsulating antigen, alone or in combination with other antigens, adjuvants, stabilizers, and release modifiers, are present in the formulations. Antigen is preferably present in excipient at the time of administration, or on the surface of the formulation, for immediate release, and incorporated within the formulation for release at ten to 45 days after initial release of antigen, optionally at ten to 90 day intervals for release of antigen in one or more additional time periods. Antigen may be stabilized through the use of stabilizing agents such as trehalose glass. In a preferred embodiment for immunization against polio, antigen is released at the time of administration, and two, four and six months thereafter.

公开(公告)号：US11608519B2

公开(公告)日：2023-03-21

申请号：US16049460

申请日：2018-07-30

申请人： TOKITAE LLC

发明人： Ted A. Baughman ,  Damian Madan ,  Eric Nalefski ,  Anne-Laure M. Le Ny

IPC分类号： C12Q1/6832 ,  C12N9/22 ,  C12N15/11

摘要： Embodiments disclosed herein include devices, methods, and systems for direct, selective, and sensitive detection of single-stranded and double-stranded target DNA sequences from various sources using a Cas12a protein. When activated by binding a target DNA sequence, the Cas12a cleaves a tether releasing a reporter molecule that may then be detected. In some embodiments, the systems, methods, and devices may include a filter or membrane that may help to separate the tethered and untethered reporter molecules. These devices, systems, and techniques allow a user to rapidly process samples that may contain the target DNA, without needing to amplify the target sequences. These devices and methods may be used to assay a wide variety of samples and target DNA sources, for the presence or absence of target DNA sequences. Compositions and kits, useful in practicing these methods, for example detecting a target DNA in a biological sample, are also described.

公开(公告)号：US11541017B2

公开(公告)日：2023-01-03

申请号：US15484892

申请日：2017-04-11

申请人： Massachusetts Institute of Technology ,  Tokitae LLC

发明人： Ana Jaklenec ,  Xian Xu ,  Lowell L. Wood, Jr. ,  Philip A. Welkhoff ,  William Gates ,  Boris Nikolic ,  Robert S. Langer

IPC分类号： A61K9/50 ,  A23L33/15 ,  A23L33/155 ,  A23L33/16 ,  A23P10/30 ,  A61K31/375 ,  A61K31/593 ,  A61K31/714 ,  A61K31/18 ,  A61K31/26 ,  A61K31/30 ,  A61K31/519 ,  A61K47/26 ,  A61K47/36 ,  A61K31/295 ,  A61K33/30 ,  A61K31/07 ,  A61K33/18 ,  A23L27/00 ,  A23L33/10 ,  A61K9/48 ,  A61K31/23 ,  A61K33/26 ,  A61K45/06

摘要： Salt formulations, which are resistant to moisture and cooking conditions, are described herein. The formulations provide particles of micronutrients and vitamins encapsulated within heat resistant pH-sensitive water-insoluble polymers, which are packaged within a salt shell. The pH-sensitive, water-insoluble, thermally stable materials stabilize the micronutrients, particularly at high temperatures, such as during food preparation and cooking, and release the micronutrients at the desired locations such as the stomach, small intestine, etc. Preferred pH-sensitive polymers release at a low pH, less than the pH present in the stomach. The particles can be used to deliver daily-recommended doses of micronutrients simultaneously with salt, eliminating the need for vitamin pills. This is particularly important in populations suffering from severe malnutrition.

公开(公告)号：US20220386993A1

公开(公告)日：2022-12-08

申请号：US17819103

申请日：2022-08-11

申请人： TOKITAE LLC

发明人： Courosh Mehanian ,  Sebastian Wachsmann-Hogiu ,  Benjamin K. Wilson ,  Xinliang Zheng

IPC分类号： A61B8/00 ,  A61B8/08 ,  G01S7/52 ,  G01S15/89

摘要： Embodiments disclosed herein are directed to systems and methods for determining if a fluid is present in a body region. The systems and methods include using ultrasound systems having operational parameters that provide ultrasound echo maps having high resolution B-line artefacts.

公开(公告)号：US11446004B2

公开(公告)日：2022-09-20

申请号：US15985808

申请日：2018-05-22

申请人： Tokitae LLC

发明人： Courosh Mehanian ,  Sebastian Wachsmann-Hogiu ,  Benjamin K. Wilson ,  Xinliang Zheng

IPC分类号： A61B8/00 ,  A61B8/08 ,  G01S7/52 ,  G01S15/89 ,  A61B8/14 ,  A61B5/00

摘要： Embodiments disclosed herein are directed to systems and methods for determining if a fluid is present in a body region. The systems and methods include using ultrasound systems having operational parameters that provide ultrasound echo maps having high resolution B-line artefacts.

公开(公告)号：US20220106647A1

公开(公告)日：2022-04-07

申请号：US17430235

申请日：2020-02-07

申请人： Tokitae LLC

发明人： Ted Baughman ,  Anne-Laure M. Le Ny ,  Philip Leung ,  Damian Madan ,  Eric Nalefski

IPC分类号： C12Q1/6897 ,  C12N9/22 ,  C12N15/11

摘要： Embodiments disclosed herein include devices, methods, and systems for direct, selective, and sensitive detection of single-stranded and double-stranded target nucleic acid sequences from various sources in a solution-based system. When activated by binding a target nucleic acid sequence, the Cas protein cleaves a tether separating a reporter molecule from a capture moiety. The capture moiety can then be used to remove, localize, or sequester uncleaved molecule containing intact tethers. In some embodiments, the systems, methods, and devices may include a filter, a membrane, or other molecules that may help to separate the tethered and untethered reporter molecules and/or capture the reporter molecules. These devices, systems, and techniques allow a user to rapidly process samples that may contain the target nucleic acid, in some cases, without needing to amplify the target sequences, and without the need for sophisticated or expensive laboratory equipment. These devices and methods may be used to assay a wide variety of samples and target nucleic acid sources, for the presence or absence of a specific target sequences. Compositions and kits, useful in practicing these methods, for example detecting a target RNA or DNA in a biological sample, are also described.

公开(公告)号：US10707683B2

公开(公告)日：2020-07-07

申请号：US15715491

申请日：2017-09-26

申请人： TOKITAE LLC

发明人： Brian L. Pal ,  Roderick T. Hinman ,  Shieng Liu ,  Jennifer Ezu Hu ,  Fridrik Larusson ,  Fong-Li Chou ,  Nels R. Peterson ,  Lowell L. Wood, Jr.

IPC分类号： G05D3/00 ,  H02J3/38 ,  H02S50/10 ,  H02J1/14 ,  G06Q50/06 ,  H02S40/38 ,  F25B49/00 ,  G05F1/67 ,  H02J3/14 ,  H02S50/00 ,  H02J7/35 ,  G05B19/04

摘要： Generally, this disclosure relates to methods of prioritizing or directing available power to a main device, such as a temperature-stabilized and/or temperature-controlled storage container. In an embodiment, the method may include measuring electrical power available from a solar photovoltaic module array that is electrically coupled to the main device, and modulating the electrical power drawn by the main device based on the available electrical power. Available power unused by the main device may be diverted to one or more secondary devices.

公开(公告)号：US20200032325A1

公开(公告)日：2020-01-30

申请号：US16049460

申请日：2018-07-30

申请人： TOKITAE LLC

发明人： Ted A. Baughman ,  Damian Madan ,  Eric Nalefski ,  Anne-Laure M. Le Ny

IPC分类号： C12Q1/6832 ,  C12N9/22 ,  C12N15/11

摘要： Embodiments disclosed herein include devices, methods, and systems for direct, selective, and sensitive detection of single-stranded and double-stranded target DNA sequences from various sources using a programmed Cas12a protein. When activated by binding a target DNA sequence, the Cas12a cleaves a tether releasing a reporter molecule that may then be detected. In some embodiments, the systems, methods, and devices may include a filter or membrane that may help to separate the tethered and untethered reporter molecules. These devices, systems, and techniques allow a user to rapidly process samples that may contain the target DNA, without needing to amplify the target sequences. These devices and methods may be used to assay a wide variety of samples and target DNA sources, for the presence or absence of a specific target DNA sequences. Compositions and kits, useful in practicing these methods, for example detecting a target DNA in a biological sample, are also described.

公开(公告)号：US20200032324A1

公开(公告)日：2020-01-30

申请号：US16049457

申请日：2018-07-30

申请人： TOKITAE LLC ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Ted A. Baughman ,  James Cate ,  Jennifer Doudna ,  Gavin John Knott ,  Damian Madan ,  Eric Nalefski ,  Anne-Laure M. Le Ny ,  Brittney Thornton

IPC分类号： C12Q1/6832 ,  C12N9/22 ,  C12N15/11

摘要： Embodiments disclosed herein include devices, methods, and systems for direct, selective, and sensitive detection of single-stranded target RNA sequences from various sources using a programmed Cas13a protein. When activated by binding a target RNA sequence, the Cas13a cleaves a tether releasing a reporter molecule that may then be detected. In some embodiments, the systems, methods, and devices may include a filter or membrane that may help to separate the tethered and untethered reporter molecules. These devices, systems, and techniques allow a user to rapidly process samples that may contain the target RNA, without needing to amplify the target sequences. These devices and methods may be used to assay a wide variety of samples and target RNA sources, for the presence or absence of a specific target RNA sequence. Compositions and kits, useful in practicing these methods, for example detecting a target RNA in a biological sample, are also described.

公开(公告)号：US20200030234A1

公开(公告)日：2020-01-30

申请号：US16528197

申请日：2019-07-31

申请人： Massachusetts Institute of Technology ,  The Brigham and Women's Hospital, Inc. ,  Tokitae LLC

发明人： Shiyi Zhang ,  Andrew Bellinger ,  Carlo Giovanni Traverso ,  Robert S. Langer ,  Dean Liang Glettig ,  Lowell L. Wood, JR. ,  Philip A. Eckhoff

IPC分类号： A61K9/00 ,  C08G18/73 ,  C08G63/08 ,  C08G18/42 ,  A61K47/58 ,  A61K47/69 ,  A61K9/48 ,  A61K31/357 ,  A61K31/65 ,  A61K31/7048 ,  C08G83/00 ,  A61K47/32 ,  C08L33/02 ,  C08L33/08 ,  C08L33/14 ,  A61K47/10 ,  A61K47/34 ,  A61K47/40 ,  A61K47/42 ,  A61M31/00

摘要： Enteric elastomers and related methods are generally provided. In some embodiments, the enteric elastomer is a polymer composite. Certain embodiments comprise a polymer composite in which hydrogen bonds within two carboxyl group-containing polymers cross-link the polymer networks into an elastic and pH-responsive polymer composite. Advantageously, this polymer composite has the capacity of being stable and elastic in an acidic environment such as that of the stomach but can be dissolved in a neutral pH environment such as that of the small and large intestines. In some embodiments, the polymer composites described herein comprise a mixture of two or more polymers with carboxyl functionality such that the two or more polymers form hydrogen bonds. In certain embodiments, the polymer composite has both enteric and elastic properties.