公开(公告)号：US20220356442A1

公开(公告)日：2022-11-10

申请号：US17812545

申请日：2022-07-14

Applicant: TOPPAN Inc.

Inventor： Yasuyuki HIRAOKA

IPC: C12N5/071

Abstract: A method of producing a three-dimensional cell structure includes producing a mixture of a cell cluster including an endothelial cell, an extracellular matrix component, and a polymer electrolyte, removing a liquid from the mixture to obtain a cell aggregate, and culturing the cell aggregate in a medium to obtain a three-dimensional cell structure with a thickness greater than 150 μm and having a vascular network. The extracellular matrix component is collagen or a collagen analog, and the polymer electrolyte is heparin or a heparin analog having a final concentration of 0.001 mg/mL or higher in the mixture.

公开(公告)号：US20240191202A1

公开(公告)日：2024-06-13

申请号：US18552007

申请日：2022-03-02

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY

Inventor： Yasuyuki HIRAOKA ,  Michiya MATSUSAKI

IPC: C12N5/071

CPC classification number: C12N5/0671 ,  C12N2501/91 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2533/56

Abstract: Disclosed is a method for producing a cell aggregate having bile canaliculi, including a contact step of bringing cells including hepatocytes into contact with heparin in an aqueous medium and a culture step of culturing the cells brought in contact with the heparin to form the cell aggregate.

公开(公告)号：US20230282008A1

公开(公告)日：2023-09-07

申请号：US18317170

申请日：2023-05-15

Applicant: TOPPAN Inc.

Inventor： Yasuyuki HIRAOKA

IPC: G06V20/69 ,  G06V10/52 ,  G06V10/24 ,  G06V10/20 ,  G06V10/26

CPC classification number: G06V20/695 ,  G06V20/693 ,  G06V20/698 ,  G06V10/52 ,  G06V10/245 ,  G06V10/255 ,  G06V10/273 ,  G06V2201/033

Abstract: An image processing method includes acquiring an image in which a cell structure having a stained vascular network structure is imaged, applying a wavelet transform to the image such that a contour image in which contours of the vascular network structure are extracted is generated, and repeatedly excluding object pixels from object boundaries recognized in the contour image such that a skeleton image in which skeletons having a line width of a predetermined number of pixels are extracted is generated.

公开(公告)号：US20220411750A1

公开(公告)日：2022-12-29

申请号：US17777786

申请日：2020-11-17

Applicant: TOPPAN INC. ,  Osaka University

Inventor： Yasuyuki HIRAOKA ,  Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12N5/071

Abstract: The present invention relates to a cell structure including cells containing at least hepatocytes and vascular endothelial cells, and extracellular matrix component, wherein the extracellular matrix components are disposed between the cells, and a liver sinusoidal network is provided between the cells.