公开(公告)号：US20220145242A1

公开(公告)日：2022-05-12

申请号：US17430648

申请日：2021-03-25

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12N5/00 ,  C07K14/78

Abstract: The present invention relates to an extracellular matrix-containing composition containing a fragmented extracellular matrix component and a compound bound or adsorbed to the fragmented extracellular matrix component.

公开(公告)号：US20220411750A1

公开(公告)日：2022-12-29

申请号：US17777786

申请日：2020-11-17

Applicant: TOPPAN INC. ,  Osaka University

Inventor： Yasuyuki HIRAOKA ,  Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12N5/071

Abstract: The present invention relates to a cell structure including cells containing at least hepatocytes and vascular endothelial cells, and extracellular matrix component, wherein the extracellular matrix components are disposed between the cells, and a liver sinusoidal network is provided between the cells.

公开(公告)号：US20230201426A1

公开(公告)日：2023-06-29

申请号：US17926924

申请日：2021-06-16

Applicant: TOPPAN INC. ,  Osaka University

Inventor： Shiro KITANO ,  Michiya MATSUSAKI ,  Hidehiro SAKURAI ,  Yuta UETAKE

IPC: A61L27/52 ,  A61L27/04 ,  A61L27/24 ,  A61L27/36

CPC classification number: A61L27/52 ,  A61L27/047 ,  A61L27/24 ,  A61L27/3633

Abstract: The present invention relates to a gel composition containing at least one selected from the group consisting of an extracellular matrix component and a fragmented extracellular matrix component, and an ion of a metal element.

公开(公告)号：US20230110381A1

公开(公告)日：2023-04-13

申请号：US17908283

申请日：2021-03-04

Applicant: OSAKA UNIVERSITY ,  TOPPAN INC.

Inventor： Michiya MATSUSAKI ,  Naoko SASAKI ,  Shiro KITANO ,  Shinji IRIE

IPC: A61L27/50 ,  A61L27/36 ,  A61L27/24 ,  A61L27/38 ,  C12N5/00

Abstract: Disclosed is a method for controlling the Young’s modulus of a three-dimensional tissue containing cells and an extracellular matrix by adjusting the average diameter of an extracellular matrix in production of the three-dimensional tissue.

公开(公告)号：US20220341918A1

公开(公告)日：2022-10-27

申请号：US17642608

申请日：2020-08-27

Applicant: TOPPAN INC. ,  Osaka University

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI ,  Naoko SASAKI ,  Atsuo AMANO ,  Hiroki TAKEUCHI

IPC: G01N33/50 ,  C12N5/071

Abstract: Provided is a cell structure including: a connective tissue structure; and an epithelial structure placed on the connective tissue structure, in which the connective tissue structure contains a fragmented extracellular matrix component and first cells including mesenchymal cells, at least a part of the fragmented extracellular matrix component is placed between the first cells, and the epithelial structure contains epithelial cells.

公开(公告)号：US20220170911A1

公开(公告)日：2022-06-02

申请号：US17600348

申请日：2020-03-19

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY ,  Kyoto Prefectural Public University Corporation

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI ,  Fiona LOUIS ,  Yoshihiro SOWA

IPC: G01N33/50 ,  C12N5/077 ,  C12N5/0775 ,  C12N5/071 ,  A61K35/35

Abstract: Disclosed is a cell structure comprising: a fragmented extracellular matrix component; and cells, wherein the cell structure comprises an intercellular vascular network, and the cells comprise at least adipocytes and vascular endothelial cells.

公开(公告)号：US20240301347A1

公开(公告)日：2024-09-12

申请号：US18578247

申请日：2022-06-29

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY

Inventor： Asuka YAMADA ,  Shiro KITANO ,  Michiya MATSUSAKI

IPC: C12N5/00

CPC classification number: C12N5/0068 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2537/00

Abstract: One aspect of the present invention relates to a production method of a fragmented extracellular matrix component, the method including: a step of neutralizing a solution in which an extracellular matrix component is dissolved; a step of obtaining a mass containing the extracellular matrix component by removing the solvent through freeze-drying treatment after the neutralization step; a step of obtaining a liquid containing the fragmented extracellular matrix component by dispersing the mass in a solution containing a polar organic solvent and fragmenting the extracellular matrix component in the solution; and a step of removing a liquid component from the liquid containing the fragmented extracellular matrix component.

公开(公告)号：US20240191202A1

公开(公告)日：2024-06-13

申请号：US18552007

申请日：2022-03-02

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY

Inventor： Yasuyuki HIRAOKA ,  Michiya MATSUSAKI

IPC: C12N5/071

CPC classification number: C12N5/0671 ,  C12N2501/91 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2533/56

Abstract: Disclosed is a method for producing a cell aggregate having bile canaliculi, including a contact step of bringing cells including hepatocytes into contact with heparin in an aqueous medium and a culture step of culturing the cells brought in contact with the heparin to form the cell aggregate.

公开(公告)号：US20240117313A1

公开(公告)日：2024-04-11

申请号：US18276912

申请日：2022-02-15

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY ,  Kyoto Prefectural Public University Corporation

Inventor： Shiro KITANO ,  Michiya MATSUSAKI ,  Fiona LOUIS ,  Yoshihiro SOWA

IPC: C12N5/077

CPC classification number: C12N5/0653 ,  C12N2500/36 ,  C12N2501/998 ,  C12N2506/1384 ,  C12N2513/00

Abstract: A method for producing a three-dimensional tissue construct comprising mature adipocytes, comprising incubating cells comprising at least fat-derived stein cells in the presence of one or more fatty acids selected from the group consisting of erucic acid, elaidic acid, oleic acid, palmitoleic acid, myristoleic acid, phytanic acid, and pristanic acid.

公开(公告)号：US20230389540A1

公开(公告)日：2023-12-07

申请号：US18250814

申请日：2021-10-15

Applicant: OSAKA UNIVERSITY ,  TOPPAN INC. ,  Kyoto Prefectural Public University Corporation

Inventor： Michiya MATSUSAKI ,  Fiona LOUIS ,  Shiro KITANO ,  Yoshihiro SOWA

IPC: A01N1/02

CPC classification number: A01N1/0284

Abstract: The present invention relates to a method for freezing a cell structure including freezing a cell structure including fragmented extracellular matrix components, cells and fibrin and having a three-dimensional tissue structure.