公开(公告)号：US20230158483A1

公开(公告)日：2023-05-25

申请号：US17920135

申请日：2021-04-23

申请人： TOYOBO CO., LTD.

发明人： Ayano Higaki ,  Hirofumi Hakuba

IPC分类号： B01J41/16 ,  B01J41/04 ,  C08J5/22 ,  B01D69/02 ,  B01J47/014

CPC分类号： B01J41/16 ,  B01J41/04 ,  C08J5/2212 ,  B01D69/02 ,  B01J47/014 ,  B01D2325/02833 ,  B01D2325/02834 ,  B01D2325/022 ,  B01D2325/04 ,  B01D2325/16 ,  B01D2325/42 ,  C08J2301/08

摘要： An object of the present invention is to provide an ion-exchange membrane for simply and inexpensively separating and purifying exosomes present in a biological sample such as serum. The invention relates to a cellulose-based ion-exchange membrane containing a cellulose-based polymer having at least one hydroxyl group or acetyl group at the 2-, 3-, or 6-position being replaced with a positively charged compound. The invention also relates to a method for purifying exosomes, including subjecting a sample containing exosomes to membrane permeation by using the cellulose-based ion-exchange membrane to allow for adsorption of the exosomes, bringing the membrane into contact with a washing liquid to remove impurities, and bringing the membrane into contact with an eluent to allow for desorption of the exosomes.