公开(公告)号：US09212376B2

公开(公告)日：2015-12-15

申请号：US13034098

申请日：2011-02-24

申请人： Tadashi Araki ,  Tomonori Hidesaki ,  Seiichi Watanabe ,  Keita Nishida ,  Kiyoteru Nagahara ,  Mitsuo Koito

发明人： Tadashi Araki ,  Tomonori Hidesaki ,  Seiichi Watanabe ,  Keita Nishida ,  Kiyoteru Nagahara ,  Mitsuo Koito

IPC分类号： C12P13/06 ,  C12N9/00 ,  C07H21/02 ,  C12N9/10

CPC分类号： C12P13/06 ,  C12N9/1014

摘要： This invention relates to DNA encoding a novel enzyme having activity of synthesizing D-serine from formaldehyde and glycine, recombinant DNA constructed by integrating such DNA into a vector, a transformant transformed with the recombinant DNA, and a method for producing D-serine from formaldehyde and glycine with the use of the enzyme.

摘要翻译： 本发明涉及编码具有从甲醛和甘氨酸合成D-丝氨酸的新型酶的DNA，通过将这种DNA整合到载体中构建的重组DNA，用重组DNA转化的转化体和从甲醛生产D-丝氨酸的方法 和甘氨酸与酶的使用。

公开(公告)号：US20120077233A1

公开(公告)日：2012-03-29

申请号：US13034098

申请日：2011-02-24

申请人： Tadashi Araki ,  Tomonori Hidesaki ,  Seiichi Watanabe ,  Keita Nishida ,  Kiyoteru Nagahara ,  Mitsuo Koito

发明人： Tadashi Araki ,  Tomonori Hidesaki ,  Seiichi Watanabe ,  Keita Nishida ,  Kiyoteru Nagahara ,  Mitsuo Koito

IPC分类号： C12P13/06

CPC分类号： C12P13/06 ,  C12N9/1014

摘要： This invention relates to DNA encoding a novel enzyme having activity of synthesizing D-serine from formaldehyde and glycine, recombinant DNA constructed by integrating such DNA into a vector, a transformant transformed with the recombinant DNA, and a method for producing D-serine from formaldehyde and glycine with the use of the enzyme.

摘要翻译： 本发明涉及编码具有从甲醛和甘氨酸合成D-丝氨酸的新型酶的DNA，通过将这种DNA整合到载体中构建的重组DNA，用重组DNA转化的转化体和从甲醛生产D-丝氨酸的方法 和甘氨酸与酶的使用。

公开(公告)号：US07919285B2

公开(公告)日：2011-04-05

申请号：US11665194

申请日：2005-10-07

申请人： Tadashi Araki ,  Tomonori Hidesaki ,  Seiichi Watanabe ,  Keita Nishida ,  Kiyoteru Nagahara ,  Mitsuo Koito

发明人： Tadashi Araki ,  Tomonori Hidesaki ,  Seiichi Watanabe ,  Keita Nishida ,  Kiyoteru Nagahara ,  Mitsuo Koito

IPC分类号： C12P13/06 ,  C12N9/00 ,  C07H21/04

CPC分类号： C12P13/06 ,  C12N9/1014

摘要： This invention relates to DNA encoding an enzyme having activity of synthesizing D-serine from formaldehyde and glycine, recombinant DNA constructed by integrating such DNA into a vector, a transformant transformed with the recombinant DNA, and a method for producing D-serine from formaldehyde and glycine with the use of the enzyme.

摘要翻译： 本发明涉及编码具有从甲醛和甘氨酸合成D-丝氨酸的酶的酶的DNA，通过将这种DNA整合到载体中构建的重组DNA，用重组DNA转化的转化体和从甲醛生产D-丝氨酸的方法，以及 甘氨酸与酶的使用。

公开(公告)号：US20080293098A1

公开(公告)日：2008-11-27

申请号：US11665194

申请日：2005-10-07

申请人： Tadashi Araki ,  Tomonori Hidesaki ,  Seiichi Watanabe ,  Keita Nishida ,  Kiyoteru Nagahara ,  Mitsuo Koito

发明人： Tadashi Araki ,  Tomonori Hidesaki ,  Seiichi Watanabe ,  Keita Nishida ,  Kiyoteru Nagahara ,  Mitsuo Koito

IPC分类号： C12P21/04 ,  C12N15/11 ,  C12N15/00 ,  C12P13/06 ,  C12N1/20 ,  C12N9/00

CPC分类号： C12P13/06 ,  C12N9/1014

摘要： This invention relates to DNA encoding a novel enzyme having activity of synthesizing D-serine from formaldehyde and glycine, recombinant DNA constructed by integrating such DNA into a vector, a transformant transformed with the recombinant DNA, and a method for producing D-serine from formaldehyde and glycine with the use of the enzyme.

摘要翻译： 本发明涉及编码具有从甲醛和甘氨酸合成D-丝氨酸的新型酶的DNA，通过将这种DNA整合到载体中构建的重组DNA，用重组DNA转化的转化体和从甲醛生产D-丝氨酸的方法 和甘氨酸与酶的使用。

公开(公告)号：US06333427B1

公开(公告)日：2001-12-25

申请号：US09507676

申请日：2000-02-22

申请人： Hitoki Miyake ,  Masami Osabe ,  Mitsuo Koito ,  Setsuo Yoshino ,  Nobuhiro Fukuhara

发明人： Hitoki Miyake ,  Masami Osabe ,  Mitsuo Koito ,  Setsuo Yoshino ,  Nobuhiro Fukuhara

IPC分类号： C07C22900

CPC分类号： C07C269/08 ,  C07B2200/07 ,  C07C269/04 ,  C07C271/22

摘要： A purified N-t-butoxycarbonylphenylalanine ester preparation having an enhanced optical activity can be obtained by bringing an N-t-butoxycarbonylphenylalanine ester preparation containing an optically active compound into contact with an aliphatic hydrocarbon, extracting the optically active compound with the aliphatic hydrocarbon, and recovering the optically active compound from the resulting extract.

摘要翻译： 具有增强的光学活性的纯化的N-叔丁氧基羰基苯丙氨酸酯制剂可以通过使含有光学活性化合物的N-叔丁氧基羰基苯丙氨酸酯制剂与脂族烃接触，用脂族烃萃取旋光活性化合物，并回收光学活性 从所得提取物中加入化合物。

公开(公告)号：US20050143581A1

公开(公告)日：2005-06-30

申请号：US10508117

申请日：2003-03-18

申请人： Hirokazu Awano ,  Mitsuo Koito ,  Masahiko Kusumoto

发明人： Hirokazu Awano ,  Mitsuo Koito ,  Masahiko Kusumoto

IPC分类号： C07D277/38 ,  C07D277/46 ,  C07D277/18

CPC分类号： C07D277/38 ,  C07D277/46

摘要： An alkoxybenzamide represented by the general formula (3): (wherein R1, R2, R3, R4, and R5 independently indicate a hydrogen atom, an alkyl group having one to six carbon atoms, or an alkoxy group having one to six carbon atoms; if R3 indicates a hydrogen atom or an alkyl group having one to six carbon atoms, R1 indicates an alkoxy group having one to six carbon atoms and R4 indicates an alkyl or alkoxy group having one to six carbon atoms; two adjacent substituents may be coupled with a cross-linking group to form a ring; R6 indicates a heteroaromatic ring; and A indicates an oxygen atom or a sulfur atom) is produced by reacting an alkoxybenzene represented by the general formula (1): (wherein R1, R2, R3, R4, and R5 are defined above) with an isocyanate represented by the general formula (2): (wherein R6 and A are defined above; and n indicates 1 or 2) in the presence of a Lewis acid. Among isocyanates represented by the general formula (2) is a thiazolyl isocyanate represented by the general formula (5): (wherein R1 indicates a hydrogen atom, a saturated or unsaturated alkyl group having one to six carbon atoms, a haloalkyl group having one to six carbon atoms, an alkoxy group having one to six carbon atoms, an alkoxycarbonyl group, or an acyl group; R2 indicates a hydrogen atom, a halogen atom, a saturated or unsaturated alkyl group having one to six carbon atoms, a haloalkyl group having one to six carbon atoms, an alkoxycarbonyl group having one to six carbon atoms, an acyl group, or a substituted or unsubstituted phenyl group; both R1 and R2 do not indicate a hydrogen atom; and n indicates 1 or 2). This thiazolyl isocyanate is produced from two precursors: an aminothiazole and a carbonyl halide. The present invention can provide a method for producing a high yield of alkoxybenzamide with fewer steps, and can also provide thiazolyl isocyanates, which are useful in the production of ureas, carbamates, and aromatic amides having a thiazole ring.