NOVEL METHOD OF DESIGNING DNA PROBE CHIP FOR ROOM TEMPERATURE HYBRIDIZATION AND THE DNA PROBE CHIP
    9.
    发明申请
    NOVEL METHOD OF DESIGNING DNA PROBE CHIP FOR ROOM TEMPERATURE HYBRIDIZATION AND THE DNA PROBE CHIP 审中-公开
    设计用于室温杂交的DNA探针芯片的新方法和DNA探针芯片

    公开(公告)号:US20090203005A1

    公开(公告)日:2009-08-13

    申请号:US12267421

    申请日:2008-11-07

    IPC分类号: C12Q1/68 C12P19/34

    摘要: The present invention relates to a method of designing DNA probe chip for room-temperature hybridization in order to solve the solvent evaporation problem occurring when carrying out said hybridization at a high temperature of 40° C.˜50° C. or higher, wherein the method is designed to allow genotyping through hybridizing at a room temperature of 20° C.˜30° C. The method of designing DNA probe chip comprises designing DNA probe to start at −10˜+5 position that is between −10 position which is overlapped 10 sequences with primer and +5 position which is 5 sequences far from the 3′-terminal of primer, based on 0 position which is 3′-terminal of primer.

    摘要翻译: 本发明涉及设计用于室温杂交的DNA探针芯片的方法,以解决在40℃〜50℃以上的高温下进行杂交时发生的溶剂蒸发问题,其中, 设计方法是通过在20°C〜30°C的室温下进行杂交进行基因分型。设计DNA探针芯片的方法包括设计DNA探针,在-10〜+ 5位置开始,位于-10位置之间 基于引物3'末端的0位,重叠10个具有引物和+5位的序列,其为远离引物3'末端的5个序列。

    Gasoline direct injection engine
    10.
    发明授权
    Gasoline direct injection engine 有权
    汽油直喷发动机

    公开(公告)号:US08701626B2

    公开(公告)日:2014-04-22

    申请号:US12869650

    申请日:2010-08-26

    IPC分类号: F02B19/14

    摘要: A gasoline direct injection engine that has an injector directly injecting fuel into a combustion chamber, a spark plug, an intake valve, and an exhaust valve and generates power by reciprocating a piston in a cylinder, may include a cavity formed on a piston head and returning some flow of the fuel injected from the injector to the intake valve, and a channel formed around the cavity.

    摘要翻译: 一种具有将燃料直接喷射到燃烧室,火花塞,进气阀和排气阀中的喷射器并通过使气缸中的活塞往复运动而产生动力的汽油直喷发动机可以包括形成在活塞头上的空腔, 将从喷射器喷射的燃料的一些流量返回到进气阀,以及在腔体周围形成的通道。