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1.发明申请Method for purification, modification and immobilization of recombinant protein 审中-公开纯化，修饰和固定重组蛋白的方法公开(公告)号：US20050112603A1
公开(公告)日：2005-05-26
申请号：US10798440
申请日：2004-03-12
Abstract: The present invention relates to a method for purifying, modifying and immobilizing recombinant protein. The method utilizes genetic engineering to tag a DNA sequence encoding a target protein with a specific tag and express the vector to obtain a recombinant protein. The recombinant protein is then purified and modified by an affinity column and modification reagent. After exchanging the recombinant protein with a decoupling reagent, the recombinant protein is immobilized onto a specific substrate. The method, combining steps of purification, modification and immobilization, provides convenience to using recombinant protein. The omission of the use of dialysis or molecular sieve leads to a shorter period dedicating for removing excessive reagents and the increase of efficiency of recombinant protein recovery.
Abstract translation: 本发明涉及一种纯化，修饰和固定重组蛋白的方法。 该方法利用遗传工程来标记编码具有特异标签的靶蛋白的DNA序列，并表达载体以获得重组蛋白。 然后通过亲和柱和修饰试剂纯化和修饰重组蛋白。 在用去耦试剂更换重组蛋白后，重组蛋白被固定在特定的底物上。 该方法，结合纯化，修饰和固定步骤，为使用重组蛋白提供了便利。 省略使用透析或分子筛导致更短的时间专用于去除过量的试剂和提高重组蛋白质回收的效率。
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公开(公告)号：US20050011797A1
公开(公告)日：2005-01-20
申请号：US10868822
申请日：2004-06-17
CPC classification number: B42D15/045
Abstract: A card with an attachment structure for a disc comprises a card and an attachment structure, in which the attachment structure is disposed on said card. The attachment structure is used to attach a disc on the surface of the card so as to form the card and the disc into one body. Thereby, a user can show his idea and feeling through words on the card and images or music in the video/audio disc.
Abstract translation: 具有用于盘的附接结构的卡包括卡和附接结构，其中附接结构设置在所述卡上。 附接结构用于将卡片的表面附着在盘上，以便将卡和盘形成一体。 因此，用户可以通过卡上的单词和视频/音频盘中的图像或音乐来显示他的想法和感觉。
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