Method for purification, modification and immobilization of recombinant protein
    1.
    发明申请
    Method for purification, modification and immobilization of recombinant protein 审中-公开
    纯化,修饰和固定重组蛋白的方法

    公开(公告)号:US20050112603A1

    公开(公告)日:2005-05-26

    申请号:US10798440

    申请日:2004-03-12

    申请人: Tai Kuo Han Lin

    发明人: Tai Kuo Han Lin

    CPC分类号: C07K1/22 C07K14/78 C12N15/62

    摘要: The present invention relates to a method for purifying, modifying and immobilizing recombinant protein. The method utilizes genetic engineering to tag a DNA sequence encoding a target protein with a specific tag and express the vector to obtain a recombinant protein. The recombinant protein is then purified and modified by an affinity column and modification reagent. After exchanging the recombinant protein with a decoupling reagent, the recombinant protein is immobilized onto a specific substrate. The method, combining steps of purification, modification and immobilization, provides convenience to using recombinant protein. The omission of the use of dialysis or molecular sieve leads to a shorter period dedicating for removing excessive reagents and the increase of efficiency of recombinant protein recovery.

    摘要翻译: 本发明涉及一种纯化,修饰和固定重组蛋白的方法。 该方法利用遗传工程来标记编码具有特异标签的靶蛋白的DNA序列,并表达载体以获得重组蛋白。 然后通过亲和柱和修饰试剂纯化和修饰重组蛋白。 在用去耦试剂更换重组蛋白后,重组蛋白被固定在特定的底物上。 该方法,结合纯化,修饰和固定步骤,为使用重组蛋白提供了便利。 省略使用透析或分子筛导致更短的时间专用于去除过量的试剂和提高重组蛋白质回收的效率。

    Method and device for image filtering
    2.
    发明授权
    Method and device for image filtering 有权
    图像滤波方法和装置

    公开(公告)号:US08488897B2

    公开(公告)日:2013-07-16

    申请号:US11954630

    申请日:2007-12-12

    IPC分类号: G06K9/40

    CPC分类号: H04N7/012 H04N7/0132

    摘要: The invention discloses a method and device for image filtering in the field of communication technologies, the method including: acquiring image data of a pixel dot of a current row to be filtered and a corresponding pixel dot of at least one adjacent row; obtaining contrast information containing a transparency value, of the pixel dot of the current row and the pixel dot of the adjacent row respectively by resolving the image data of the pixel dot of the current row and the pixel dot of the adjacent row; generating a filter coefficient according to the transparency value of the pixel dot of the current row and the transparency value of the corresponding pixel dot of the adjacent row; and performing filtering on the pixel dot of the current row with the filter coefficient. The embodiments of the invention effectively avoid image flickering due to a difference between contrasts by adopting the method of at-least-two-row vertical filtering, and by generating the filter coefficients according to the transparencies in filtering.

    摘要翻译: 本发明公开了一种在通信技术领域中图像滤波的方法和装置,该方法包括:获取要滤波的当前行的像素点的图像数据和至少一个相邻行的对应像素点; 通过解析当前行的像素点和相邻行的像素点的图像数据,分别获得包含当前行的像素点和相邻行的像素点的透明度值的对比度信息; 根据当前行的像素点的透明度值和相邻行的对应像素点的透明度值生成滤波器系数; 并用滤波器系数对当前行的像素点进行滤波。 本发明的实施例通过采用至少两行垂直滤波的方法,并且通过根据滤波器中的透明度生成滤波器系数,有效地避免了由于对比度之间的差异而导致的图像闪烁。

    Restoring corrupted audio signals
    3.
    发明授权
    Restoring corrupted audio signals 有权
    恢复损坏的音频信号

    公开(公告)号:US08335579B2

    公开(公告)日:2012-12-18

    申请号:US11916446

    申请日:2006-06-15

    申请人: Han Lin

    发明人: Han Lin

    IPC分类号: G06F17/00

    摘要: A method of restoring a corrupted audio signal includes the steps of inputting the corrupted audio signal in a first channel, inputting one or more further correlated audio signals in one or more further channels, and restoring the corrupted audio signal using a Multi-Channel Autoregressive (AR) Model that models the corrupted signal as a linear combination of scaled time shifted portions of the further signal(s) and the corrupted signal. Embodiments are described in which the method is used to improve received audio signals in DAB receivers and mobile telephones.

    摘要翻译: 恢复损坏的音频信号的方法包括以下步骤:在第一通道中输入损坏的音频信号,在一个或多个另外的通道中输入一个或多个另外相关的音频信号,以及使用多声道自回归( AR)将损坏的信号建模为进一步信号的缩放的时间偏移部分和被破坏的信号的线性组合的模型。 描述了使用该方法来改善DAB接收机和移动电话中的接收音频信号的实施例。

    System for computer user interface
    4.
    发明授权
    System for computer user interface 有权
    计算机用户界面系统

    公开(公告)号:US06703999B1

    公开(公告)日:2004-03-09

    申请号:US09709721

    申请日:2000-11-13

    IPC分类号: G09G508

    摘要: A computer user interface for use in a transport vehicle (10), which defines a virtual control panel in the upper open area of the vehicle steering wheel (12). The user enters inputs by action in appropriate sections of the virtual control panel area. A computer controlled display (20) illustrates a map to the user of locations of sections in the virtual control panel area, and information that can be input by user action with particular sections. A sensor (22) monitors the virtual control panel area, and produces electronic information corresponding to user action in the virtual control panel area. Software logic in a computer (46) analyzes the electronic information to determine if there has been user action and the location thereof, for designating an action as a particular user input. Based upon the designation, the computer produces an appropriate output, such as operating or adjusting vehicle systems.

    摘要翻译: 一种在运输车辆(10)中使用的计算机用户界面,其在车辆方向盘(12)的上部敞开区域中限定虚拟控制面板。 用户通过动作在虚拟控制面板区域的适当部分输入输入。 计算机控制显示器(20)示出了向用户映射虚拟控制面板区域中的部分的位置,以及可以通过用户对特定部分的动作输入的信息。 传感器(22)监视虚拟控制面板区域,并产生与虚拟控制面板区域中的用户动作对应的电子信息。 计算机(46)中的软件逻辑分析电子信息以确定是否存在用户动作及其位置,用于指定作为特定用户输入的动作。 根据指定,计算机产生适当的输出,例如操作或调整车辆系统。

    Method of fabricating self-aligned contact
    5.
    发明授权
    Method of fabricating self-aligned contact 失效
    自对准接触的方法

    公开(公告)号:US6107175A

    公开(公告)日:2000-08-22

    申请号:US27844

    申请日:1998-02-23

    CPC分类号: H01L21/76897 H01L21/28525

    摘要: A method of a method of fabricating a contact. A substrate having a plurality of gates and a plurality of lightly doped source/drain regions is provided. A dielectric layer is formed and patterned to form a self-align contact window to expose a first lightly doped source/drain region of said lightly doped source/drain regions, and to form a first spacer on a side wall of a first gate of said gates simultaneously. An ion implantation is performed by using the first spacer as a mask, so that a first heavily doped source/drain region is formed in the first lightly doped source/drain region. A doped poly-silicon layer is formed over the substrate, and a metal silicide layer is formed on the doped poly-silicon layer. The doped poly-silicon and the metal silicide layer are patterned to form a self-align contact.

    摘要翻译: 一种制造触点的方法的方法。 提供具有多个栅极和多个轻掺杂源极/漏极区域的衬底。 形成介电层并图案化以形成自对准接触窗口,以暴露所述轻掺杂源极/漏极区域的第一轻掺杂源极/漏极区域,并在所述第一栅极的侧壁上形成第一间隔物 同时门。 通过使用第一间隔件作为掩模来执行离子注入,使得在第一轻掺杂源极/漏极区域中形成第一重掺杂源极/漏极区域。 在衬底上形成掺杂多晶硅层,并且在掺杂多晶硅层上形成金属硅化物层。 将掺杂的多晶硅和金属硅化物层图案化以形成自对准接触。

    Drone
    6.
    外观设计
    Drone 有权

    公开(公告)号:USD1048209S1

    公开(公告)日:2024-10-22

    申请号:US29930471

    申请日:2024-02-29

    申请人: Han Lin

    设计人: Han Lin

    摘要: FIG. 1 is a top perspective view of a drone, showing my new design;
    FIG. 2 is a bottom perspective view thereof;
    FIG. 3 is a front view thereof;
    FIG. 4 is a rear view thereof;
    FIG. 5 is a left side view thereof;
    FIG. 6 is a right side view thereof;
    FIG. 7 is a top view thereof; and,
    FIG. 8 is a bottom view thereof.
    The broken lines in the drawings illustrate the portions of the drone, which form no part of the claimed design.

    Camping trailer
    7.
    外观设计

    公开(公告)号:USD991613S1

    公开(公告)日:2023-07-04

    申请号:US29873606

    申请日:2023-04-04

    申请人: Han Lin

    设计人: Han Lin

    摘要: FIG. 1 is a front, left and top perspective view of a camping trailer, showing my new design;
    FIG. 2 is a rear, right and bottom perspective view thereof;
    FIG. 3 is a front view thereof;
    FIG. 4 is a rear view thereof;
    FIG. 5 is a left side view thereof;
    FIG. 6 is a right side view thereof;
    FIG. 7 is a top plan view thereof;
    FIG. 8 is a bottom plan view thereof;
    FIG. 9 is an enlarged view of portion of FIG. 1 thereof;
    FIG. 10 is an enlarged view of portion of FIG. 1 thereof;
    FIG. 11 is an enlarged view of portion of FIG. 1 thereof; and,
    FIG. 12 is a perspective view in the using configuration of FIG. 1 thereof.
    The dot-dash broken lines depict the boundaries of the enlargements that form no part of the claimed design,

    Restoring Corrupted Audio Signals
    8.
    发明申请
    Restoring Corrupted Audio Signals 有权
    恢复损坏的音频信号

    公开(公告)号:US20080187153A1

    公开(公告)日:2008-08-07

    申请号:US11916446

    申请日:2006-03-17

    申请人: Han Lin

    发明人: Han Lin

    IPC分类号: H04B1/10 H04B1/62 H04B14/00

    摘要: A method of restoring a corrupted audio signal comprises the steps of; inputting the corrupted audio signal in a first channel (30-32), inputting one or more further correlated audio signals in one or more further channels (33-35), and restoring the corrupted audio signal (36) using a Multi-Channel Autoregressive (AR) Model that models the corrupted signal as a linear combination of scaled time shifted portions of the further signal (s) and the corrupted signal. Embodiments are described in which the method is used to improve received audio signals in DAB receivers and mobile telephones.

    摘要翻译: 恢复损坏的音频信号的方法包括以下步骤: 在第一通道(30-32)中输入损坏的音频信号,在一个或多个另外的通道(33-35)中输入一个或多个进一步相关的音频信号,以及使用多通道自回归的方式恢复损坏的音频信号 (AR)模型,其将损坏的信号建模为进一步信号的缩放的时间偏移部分和损坏的信号的线性组合。 描述了使用该方法来改善DAB接收机和移动电话中的接收音频信号的实施例。

    APPARATUS AND METHOD OF FORMING METAL OXIDE SEMICONDUCTOR FIELD-EFFECT TRANSISTOR WITH ATOMIC LAYER DEPOSITED GATE DIELECTRIC
    9.
    发明申请
    APPARATUS AND METHOD OF FORMING METAL OXIDE SEMICONDUCTOR FIELD-EFFECT TRANSISTOR WITH ATOMIC LAYER DEPOSITED GATE DIELECTRIC 审中-公开
    用原子层沉积栅介质形成金属氧化物半导体场效应晶体管的装置和方法

    公开(公告)号:US20080048216A1

    公开(公告)日:2008-02-28

    申请号:US11753993

    申请日:2007-05-25

    申请人: Peide Ye Yi Xuan Han Lin

    发明人: Peide Ye Yi Xuan Han Lin

    IPC分类号: H01L21/335 H01L29/78

    CPC分类号: H01L29/517 H01L21/28264

    摘要: A method for forming a metal oxide semiconductor field-effect transistor (MOSFET) includes forming a III-V compound semiconductor on a substrate with the III-V compound semiconductor being doped with a first dopant type. The method further includes doping a first and second region of the III-V compound semiconductor with a second dopant type to form a drain and a source of the MOSFET. The method further includes forming a gate dielectric on the III-V compound semiconductor through atomic layer deposition. The method further includes applying a metal onto the dielectric to form a gate of the MOSFET. A MOSFET is also disclosed herein.

    摘要翻译: 一种用于形成金属氧化物半导体场效应晶体管(MOSFET)的方法包括在III-V族化合物半导体掺杂有第一掺杂剂型的基板上形成III-V族化合物半导体。 该方法还包括用第二掺杂剂掺杂III-V族化合物半导体的第一和第二区域以形成MOSFET的漏极和源极。 该方法还包括通过原子层沉积在III-V族化合物半导体上形成栅极电介质。 该方法还包括将金属施加到电介质上以形成MOSFET的栅极。 这里也公开了一种MOSFET。

    Scooter
    10.
    发明授权
    Scooter 失效
    摩托车

    公开(公告)号:US06343798B1

    公开(公告)日:2002-02-05

    申请号:US09677755

    申请日:2000-10-03

    申请人: Shou Mao Chen Han Lin

    发明人: Shou Mao Chen Han Lin

    IPC分类号: B62M100

    CPC分类号: B62K15/006 B62K3/002 B62M1/00

    摘要: A scooter including a footboard, a rear wheel, a front wheel rod fastened to the front end of the footboard in conjunction with a folding member, a handlebar fastened to the top end of the front wheel rod, a wheel frame fastened to the bottom end of the front wheel rod, and a front wheel member fastened pivotally to the wheel frame and formed of three front wheels to stabilize the scooter in motion. Two of the three front wheels are in contact with the ground surface, with the remaining one front wheel being pivoted over the two front wheels such that the remaining one front wheel is capable of swiveling to come in contact with the ground surface at the time when one of the two front wheels encounters an obstacle. The folding member enables the front wheel rod to swivel toward the footboard so as to facilitate the storage or transportation of the scooter.

    摘要翻译: 一种踏板车,包括脚踏板,后轮,与折叠构件相结合地固定在脚踏板前端的前轮杆,固定在前轮杆顶端的把手,固定在底端的轮架 和前轮构件,该前轮构件枢转地固定到车轮框架并由三个前轮形成以使踏板车运动稳定。 三个前轮中的两个与地面接触,其余一个前轮在两个前轮上枢转,使得剩余的一个前轮能够旋转以与地面接触, 两个前轮之一遇到障碍物。 折叠构件使得前轮杆能够朝向脚踏板转动,以便于踏板车的储存或运输。