公开(公告)号：US20190113457A1

公开(公告)日：2019-04-18

申请号：US16091068

申请日：2017-07-20

申请人： TAKARA BIO USA, INC.

发明人： Patricio A. Espinoza Vallejos ,  Syed A. Husain ,  Chun-Wah LIN ,  Hermann HUBSCHLE

IPC分类号： G01N21/64 ,  B01L3/00 ,  C12Q1/6844 ,  G06K9/00

摘要： Provided are methods, devices, assemblies, and systems for dispensing into the wells of a multi-well device and imaging such wells from multiple Z-planes. Multi-Z imaging of the present methods and systems may allow for the detection of wells of a multi-well device that contain a desired number of cells. Also provided are methods, devices, assemblies, and systems for processing cell-containing wells of a multi-well device identified through the use of multi-Z imaging.

公开(公告)号：US20240173712A1

公开(公告)日：2024-05-30

申请号：US18519715

申请日：2023-11-27

申请人： Takara Bio USA, Inc.

发明人： Bradley L. Griswold ,  Mike Slater ,  Patricio A. Espinoza Vallejos ,  Tiffany M. Ding ,  Zhiliang Wan

IPC分类号： B01L3/02

CPC分类号： B01L3/0227 ,  B01L3/0237 ,  B01L2200/0642 ,  B01L2200/0689 ,  B01L2400/0478

摘要： Liquid dispensing devices are provided. Aspects of the devices include an actuator comprising a movable shaft; an elongated body, e.g., a cylinder, comprising a central channel, a proximal end and a distal end, wherein the distal end comprises an orifice; and a plunger comprising a proximal end and a distal end, wherein: the proximal end is mechanically coupled to the movable shaft, and at least a portion of the distal end of the plunger is moveably positioned within the central channel. Also provided are systems that incorporate the liquid handling device, which may be present as an array of liquid handling devices, as well as methods of using the devices and systems, e.g., in liquid dispensing applications.

公开(公告)号：US11460405B2

公开(公告)日：2022-10-04

申请号：US16091068

申请日：2017-07-20

申请人： TAKARA BIO USA, INC.

发明人： Patricio A. Espinoza Vallejos ,  Syed A. Husain ,  Chun-Wah Lin ,  Hermann Hubschle

IPC分类号： G01N21/64 ,  B01J19/00 ,  B01L7/00 ,  G01N15/14 ,  G01N21/25 ,  G06T11/00 ,  G06T7/00 ,  G06V20/69 ,  B01L3/00 ,  C12Q1/6844

摘要： Provided are methods, devices, assemblies, and systems for dispensing into the wells of a multi-well device and imaging such wells from multiple Z-planes. Multi-Z imaging of the present methods and systems may allow for the detection of wells of a multi-well device that contain a desired number of cells. Also provided are methods, devices, assemblies, and systems for processing cell-containing wells of a multi-well device identified through the use of multi-Z imaging.

公开(公告)号：US20170320038A1

公开(公告)日：2017-11-09

申请号：US15661528

申请日：2017-07-27

申请人： Takara Bio USA, Inc.

发明人： Syed A. Husain ,  Bradley L. Griswold ,  Michael Slater ,  Patricio A. Espinoza Vallejos ,  Jude Dunne ,  Glenn Hein ,  Maithreyan Srinivasan

IPC分类号： B01J19/00 ,  B01L3/00 ,  G01N1/40

CPC分类号： B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00709 ,  B01J2219/00718 ,  B01L3/502761 ,  B01L3/50857 ,  B01L2200/025 ,  B01L2200/0668 ,  B01L2300/0861 ,  B01L2300/0893 ,  B01L2300/165 ,  B01L2400/049 ,  G01N1/405

摘要： The present invention provides methods, systems, assemblies, and articles for capturing single cells with a capture chip. In certain embodiments, the capture chip comprises a substrate comprising a plurality of cell-sized dimples or wells that each allow a single cell to be captured from a cell suspension. In some embodiments, the dimples or wells of the capture chip align with the holes or wells of a multi-well through-hole chip, and/or a multi-well chip, such that the cell, or the contents of the single cell, may be transferred to a corresponding well of the multi-well chip. In particular embodiments, the bottom of each dimple or well of the capture chip has a positive electrical charge sufficient to attract cells from a cell suspension flowing over the dimples or wells.