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    METHOD FOR PRODUCING LACTIC ACID FROM PLANT-DERIVED RAW MATERIAL, AND LACTIC-ACID-PRODUCING BACTERIUM
    3.
    发明申请
    METHOD FOR PRODUCING LACTIC ACID FROM PLANT-DERIVED RAW MATERIAL, AND LACTIC-ACID-PRODUCING BACTERIUM 有权
    从植物衍生的原料生产柠檬酸的方法和生产酸乳杆菌的方法

    公开(公告)号：US20110171704A1

    公开(公告)日：2011-07-14

    申请号：US13063929

    申请日：2009-09-11

    申请人： Takashi Morishige ,  Katsuyuki Takahashi ,  Hitoshi Takahashi ,  Mitsufumi Wada ,  Daisuke Mochizuki ,  Daisuke Miyazawa ,  Tadashi Araki

    发明人： Takashi Morishige ,  Katsuyuki Takahashi ,  Hitoshi Takahashi ,  Mitsufumi Wada ,  Daisuke Mochizuki ,  Daisuke Miyazawa ,  Tadashi Araki

    IPC分类号： C12P7/56 ,  C12N1/21

    CPC分类号： C12P7/56 ,  C12N1/20 ,  C12N9/1205 ,  C12N9/2451

    摘要： The present invention provides: a lactic acid-producing Escherichia coli comprising at least one gene of a sucrose non-PTS gene group, including at least a sucrose hydrolase gene, provided that a combination of a repressor protein (cscR), a sucrose hydrolase (cscA), a fructokinase (cscK) and a sucrose permease (cscB) and a combination of a sucrose hydrolase (cscA), a fructokinase (cscK) and a sucrose permease (cscB) are excluded, wherein the lactic acid-producing Escherichia coli comprises a lactic acid production enhancing system provided by genetic recombination; and a lactic acid production method including producing lactic acid from a plant-derived sucrose-containing raw material by using the lactic acid-producing Escherichia coli.

    摘要翻译： 本发明提供：产生乳酸的大肠杆菌，其包含至少一个蔗糖非PTS基因基因的至少一个基因，至少包含蔗糖水解酶基因，前提是阻遏蛋白（cscR），蔗糖水解酶（ cscA），果糖激酶（cscK）和蔗糖通透酶（cscB）以及蔗糖水解酶（cscA），果糖激酶（cscK）和蔗糖通透酶（cscB）的组合，其中产生乳酸的大肠杆菌包含 通过遗传重组提供的乳酸生产增强系统; 以及乳酸生产方法，其包括通过使用产生乳酸的大肠杆菌从植物来源的含蔗糖的原料生产乳酸。

    Biocatalyst for production of d-lactic acid (as amended)
    5.
    发明申请
    Biocatalyst for production of d-lactic acid (as amended) 有权
    用于生产d-乳酸的生物催化剂（经修订）

    公开(公告)号：US20070065930A1

    公开(公告)日：2007-03-22

    申请号：US10573813

    申请日：2004-09-17

    申请人： Mitsufumi Wada ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

    发明人： Mitsufumi Wada ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

    IPC分类号： C12P7/56 ,  C12N1/21

    CPC分类号： C12P7/56

    摘要： A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of Escherichia coli-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector. A microorganism in which a dld gene is substantially inactivated or decreased is cultured to produce high quality D-lactic acid with reduced concentration of pyruvic acid.

    摘要翻译： 提供高产率D-乳酸的制造方法，提供高纯度的高纯度D-副乳酸和副产物有机酸的生产方法。 在一个方面，培养其中丙酮酸甲酸裂合酶（pfl）的活性失活或降低，并且进一步增强大肠杆菌来源的NADH依赖性D-乳酸脱氢酶（ldhA）的活性的微生物，以有效地产生D- 乳酸。 关于增强ldhA活性的方法，通过在基因组上连接编码ldhA的基因与控制糖酵解途径参与蛋白质的表达的基因的启动子，核酸生物合成途径或氨基酸生物合成途径 与使用表达载体增强基因表达的方法相比，获得了合适的结果。 培养其中dld基因基本上失活或减少的微生物被培养以产生具有降低的丙酮酸浓度的高质量D-乳酸。

    Process for Producng Hydroxycarboxylic Acid
    8.
    发明申请
    Process for Producng Hydroxycarboxylic Acid 有权
    生产羟基羧酸的方法

    公开(公告)号：US20080032370A1

    公开(公告)日：2008-02-07

    申请号：US11587806

    申请日：2005-04-19

    申请人： Mitsufumi Wada ,  Daisuke Mochizuki ,  Hitoshi Takahashi ,  Takashi Morishige ,  Katsuyuki Takahashi

    发明人： Mitsufumi Wada ,  Daisuke Mochizuki ,  Hitoshi Takahashi ,  Takashi Morishige ,  Katsuyuki Takahashi

    IPC分类号： C12P7/42 ,  C07C59/00

    CPC分类号： C12P7/42

    摘要： Hydroxycarboxylic acids including glycolic acid are promising compounds for use as a raw material for polymers or an intermediate for medicines. However, the processes for the production of hydroxycarboxylic acids which are presently known have a problem that none of them can supply an inexpensive high-purity hydroxycarboxylic acid. An object of the invention is to produce a microorganism which highly yields in a short time a hydroxycarboxylic acid reduced in impurity content and to provide a process for producing a hydroxycarboxylic acid including glycolic acid using the microorganism. A microorganism is produced, which has the imparted or enhanced activity of an enzyme catalyzing a reaction from ethylene glycol to glycolaldehyde. Using this microorganism, a hydroxycarboxylic acid including glycolic acid which is reduced in impurity content is highly produced in a short time. The production process enables a hydroxycarboxylic acid having high purity to be supplied at low cost.

    摘要翻译： 包括乙醇酸在内的羟基羧酸是用作聚合物或药物中间体的原料的有希望的化合物。 然而，目前已知的用于生产羟基羧酸的方法具有这样的问题，即它们都不能提供廉价的高纯度羟基羧酸。 本发明的目的是生产一种微生物，其在短时间内高度地产生杂质含量降低的羟基羧酸，并提供使用微生物生产包括乙醇酸的羟基羧酸的方法。 产生微生物，其具有赋予或增强的催化从乙二醇到乙二醇的反应的酶的活性。 使用该微生物，在短时间内高度地产生包含杂质含量降低的乙醇酸的羟基羧酸。 制造方法能够以低成本供给高纯度的羟基羧酸。