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1.发明授权Method for producing hydroxycarboxylic acid by enhancing synthesis of coenzyme 有权通过增强辅酶的合成生产羟基羧酸的方法公开(公告)号：US09133444B2
公开(公告)日：2015-09-15
申请号：US13618898
申请日：2012-09-14
CPC分类号： C12N9/1077 , C12P7/42 , C12Y204/02011
摘要： Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to produce nicotinamide adenine dinucleotide by deleting, mutating or substituting nadR gene in the microorganism or introducing a gene encoding nicotinic acid phosphoribosyltransferase.
摘要翻译： 通过使用通过在微生物中缺失，突变或取代nadR基因或引入编码烟酸磷酸核糖转移酶的基因来提高生产烟酰胺腺嘌呤二核苷酸能力的微生物来产生羟基羧酸。
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公开(公告)号：US20070065930A1
公开(公告)日：2007-03-22
申请号：US10573813
申请日：2004-09-17
申请人： Mitsufumi Wada , Toshihiro Oikawa , Daisuke Mochizuki , Junko Tokuda , Miyuki Kawashima , Tadashi Araki , Reiko Abe , Hitoki Miyake , Hitoshi Takahashi , Hideki Sawai , Takashi Mimitsuka , Takashi Morishige , Yosuke Higashi
发明人： Mitsufumi Wada , Toshihiro Oikawa , Daisuke Mochizuki , Junko Tokuda , Miyuki Kawashima , Tadashi Araki , Reiko Abe , Hitoki Miyake , Hitoshi Takahashi , Hideki Sawai , Takashi Mimitsuka , Takashi Morishige , Yosuke Higashi
CPC分类号： C12P7/56
摘要： A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of Escherichia coli-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector. A microorganism in which a dld gene is substantially inactivated or decreased is cultured to produce high quality D-lactic acid with reduced concentration of pyruvic acid.
摘要翻译： 提供高产率D-乳酸的制造方法，提供高纯度的高纯度D-副乳酸和副产物有机酸的生产方法。 在一个方面，培养其中丙酮酸甲酸裂合酶（pfl）的活性失活或降低，并且进一步增强大肠杆菌来源的NADH依赖性D-乳酸脱氢酶（ldhA）的活性的微生物，以有效地产生D- 乳酸。 关于增强ldhA活性的方法，通过在基因组上连接编码ldhA的基因与控制糖酵解途径参与蛋白质的表达的基因的启动子，核酸生物合成途径或氨基酸生物合成途径 与使用表达载体增强基因表达的方法相比，获得了合适的结果。 培养其中dld基因基本上失活或减少的微生物被培养以产生具有降低的丙酮酸浓度的高质量D-乳酸。
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公开(公告)号：US20130029392A1
公开(公告)日：2013-01-31
申请号：US13618898
申请日：2012-09-14
IPC分类号： C12P7/42
CPC分类号： C12N9/1077 , C12P7/42 , C12Y204/02011
摘要： Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to produce nicotinamide adenine dinucleotide by deleting, mutating or substituting nadR gene in the microorganism or introducing a gene encoding nicotinic acid phosphoribosyltransferase.
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公开(公告)号：US20090305368A1
公开(公告)日：2009-12-10
申请号：US12299679
申请日：2007-04-27
CPC分类号： C12P7/42 , C12N9/0006 , C12N9/0036
摘要： Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to regenerate oxidized-type nicotinamide adenine dinucleotide by being provided with an enhanced NADH dehydrogenase function by introducing a gene encoding NADH dehydrogenase into a microorganism.
摘要翻译： 通过使用通过将编码NADH脱氢酶的基因导入微生物而提供增强的NADH脱氢酶功能而提高氧化型烟酰胺腺嘌呤二核苷酸再生能力的微生物来制备羟基羧酸。
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公开(公告)号：US08748157B2
公开(公告)日：2014-06-10
申请号：US12299679
申请日：2007-04-27
CPC分类号： C12P7/42 , C12N9/0006 , C12N9/0036
摘要： Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to regenerate oxidized-type nicotinamide adenine dinucleotide by being provided with an enhanced NADH dehydrogenase function by introducing a gene encoding NADH dehydrogenase into a microorganism.
摘要翻译： 通过使用通过将编码NADH脱氢酶的基因导入微生物而提供增强的NADH脱氢酶功能而提高氧化型烟酰胺腺嘌呤二核苷酸再生能力的微生物来制备羟基羧酸。
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6.发明申请METHOD FOR PRODUCING HYDROXYCARBOXYLIC ACID BY ENHANCING SYNTHESIS OF COENZYME 审中-公开通过增强聚苯乙烯的合成生产羟基羧酸的方法公开(公告)号：US20090221045A1
公开(公告)日：2009-09-03
申请号：US12299646
申请日：2007-04-27
CPC分类号： C12N9/1077 , C12P7/42 , C12Y204/02011
摘要： Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to produce nicotinamide adenine dinucleotide by deleting, mutating or substituting nadR gene in the microorganism or introducing a gene encoding nicotinic acid phosphoribosyltransferase.
摘要翻译： 通过使用通过在微生物中缺失，突变或取代nadR基因或引入编码烟酸磷酸核糖转移酶的基因来提高生产烟酰胺腺嘌呤二核苷酸能力的微生物来制备羟基羧酸。
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公开(公告)号：US08728780B2
公开(公告)日：2014-05-20
申请号：US11587806
申请日：2005-04-19
申请人： Mitsufumi Wada , Daisuke Mochizuki , Hitoshi Takahashi , Takashi Morishige , Katsuyuki Takahashi
发明人： Mitsufumi Wada , Daisuke Mochizuki , Hitoshi Takahashi , Takashi Morishige , Katsuyuki Takahashi
IPC分类号： C12P7/42
CPC分类号： C12P7/42
摘要： An object of the invention is to produce a microorganism which yields high amounts in a short time of a hydroxycarboxylic acid reduced in impurity content. This invention further provides a process for producing a hydroxycarboxylic acid, including glycolic acid, using the microorganism. This process enables a hydroxycarboxylic acid having high purity to be supplied at low cost.
摘要翻译： 本发明的目的是产生微生物，其在短时间内在杂质含量降低的羟基羧酸中产生大量的量。 本发明还提供使用该微生物生产羟基羧酸（包括乙醇酸）的方法。 该方法能够以低成本提供具有高纯度的羟基羧酸。
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8.发明申请BACTERIUM CAPABLE OF PRODUCING LACTIC ACID, AND METHOD FOR PRODUCING LACTIC ACID 有权生产酸乳酸的细菌和生产酸乳酸的方法公开(公告)号：US20110171703A1
公开(公告)日：2011-07-14
申请号：US13063923
申请日：2009-09-11
申请人： Mitsufumi Wada , Katsuyuki Takahashi , Takashi Morishige , Daisuke Miyazawa , Hitoshi Takahashi , Daisuke Mochizuki , Tadashi Araki
发明人： Mitsufumi Wada , Katsuyuki Takahashi , Takashi Morishige , Daisuke Miyazawa , Hitoshi Takahashi , Daisuke Mochizuki , Tadashi Araki
CPC分类号： C12P7/56 , C12N9/0006 , C12N9/0069 , C12P41/002
摘要： The present invention provides: a lactic acid-producing Escherichia coli including an enzymatic activity of at least one NAD-dependent lactate dehydrogenase and an enzymatic activity of at least one NAD-independent lactate oxidoreductase, both of which are enhanced so as to decompose one of D-lactic acid or L-lactic acid and to produce the other one of D-lactic acid or L-lactic acid; and a lactic acid production method using the lactic acid-producing Escherichia coli.
摘要翻译： 本发明提供：具有至少一种NAD依赖性乳酸脱氢酶的酶活性和至少一种不依赖于NAD的乳酸氧化还原酶的酶活性的产生乳酸的大肠杆菌，两者都被增强以分解其中之一 D-乳酸或L-乳酸，并产生D-乳酸或L-乳酸中的另一种; 以及使用产乳酸的大肠杆菌的乳酸的制造方法。
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9.发明授权公开(公告)号：US08679800B2
公开(公告)日：2014-03-25
申请号：US13063929
申请日：2009-09-11
申请人： Takashi Morishige , Katsuyuki Takahashi , Hitoshi Takahashi , Mitsufumi Wada , Daisuke Mochizuki , Daisuke Miyazawa , Tadashi Araki
发明人： Takashi Morishige , Katsuyuki Takahashi , Hitoshi Takahashi , Mitsufumi Wada , Daisuke Mochizuki , Daisuke Miyazawa , Tadashi Araki
CPC分类号： C12P7/56 , C12N1/20 , C12N9/1205 , C12N9/2451
摘要： The present invention provides: a lactic acid-producing Escherichia coli comprising at least one gene of a sucrose non-PTS gene group, including at least a sucrose hydrolase gene, provided that a combination of a repressor protein (cscR), a sucrose hydrolase (cscA), a fructokinase (cscK) and a sucrose permease (cscB) and a combination of a sucrose hydrolase (cscA), a fructokinase (cscK) and a sucrose permease (cscB) are excluded, wherein the lactic acid-producing Escherichia coli comprises a lactic acid production enhancing system provided by genetic recombination; and a lactic acid production method including producing lactic acid from a plant-derived sucrose-containing raw material by using the lactic acid-producing Escherichia coli.
摘要翻译： 本发明提供：产生乳酸的大肠杆菌，其包含至少一个蔗糖非PTS基因组的至少一个基因，至少包含蔗糖水解酶基因，前提是阻遏蛋白（cscR），蔗糖水解酶（ cscA），果糖激酶（cscK）和蔗糖通透酶（cscB）以及蔗糖水解酶（cscA），果糖激酶（cscK）和蔗糖通透酶（cscB）的组合，其中产生乳酸的大肠杆菌包含 通过遗传重组提供的乳酸生产增强系统; 以及乳酸生产方法，其包括通过使用产生乳酸的大肠杆菌从植物来源的含蔗糖的原料生产乳酸。
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10.发明申请公开(公告)号：US20100311135A1
公开(公告)日：2010-12-09
申请号：US12452564
申请日：2008-07-04
申请人： Nozomi Takebayashi , Mitsufumi Wada , Daisuke Mochizuki , Fuminobu Yoshimi , Seiichi Watanabe , Hitoshi Takahashi , Takashi Morishige
发明人： Nozomi Takebayashi , Mitsufumi Wada , Daisuke Mochizuki , Fuminobu Yoshimi , Seiichi Watanabe , Hitoshi Takahashi , Takashi Morishige
CPC分类号： C12P7/04 , C12M21/12 , C12M47/10 , C12M47/20 , C12N9/0006 , C12N9/1029 , C12N9/13 , C12N9/88
摘要： The invention provides: an isopropyl alcohol-producing bacterium which has an acetoacetate decarboxylase activity, an isopropyl alcohol dehydrogenase activity, a CoA transferase activity and a thiolase activity having been imparted thereto and is capable of producing isopropyl alcohol from a plant-derived material; a method of producing isopropyl alcohol whereby isopropyl alcohol is produced from a plant-derived material by using this isopropyl alcohol-producing bacterium; and an apparatus therefor.
摘要翻译： 本发明提供：具有乙酰乙酸脱羧酶活性，异丙醇脱氢酶活性，CoA转移酶活性和硫硫酸酶活性的异丙醇生产细菌，并且能够从植物来源的材料生产异丙醇; 通过使用该异丙醇生产菌从植物来源的材料制造异丙醇的方法; 及其装置。
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