公开(公告)号：US20060008857A1

公开(公告)日：2006-01-12

申请号：US10530886

申请日：2003-10-09

申请人： Tamaki Oda ,  Hideki Endoh ,  Yoshitaka Ueda ,  Kazunori Inabe

发明人： Tamaki Oda ,  Hideki Endoh ,  Yoshitaka Ueda ,  Kazunori Inabe

IPC分类号： G01N33/574 ,  C07K16/30

CPC分类号： C07K14/4705 ,  C07K14/47

摘要： A novel polypeptide which is useful in screening of an agent for improving insulin resistance and an agent for improving glucose metabolism, a polynucleotide encoding the polypeptide, an expression vector comprising the polynucleotide, and a cell transformed with the expression vector are disclosed. The polypeptide is a protein expressed in skeletal muscle. When the protein is overexpressed, incorporation of sugar into a cell is inhibited. A method for screening of an agent for improving insulin resistance and/or an agent for improving glucose metabolism in which the polypeptide is used, and a method for producing a pharmaceutical composition for insulin resistance and/or glucose metabolism improvement comprising a substance obtained according to the method for screening as an active ingredient are also disclosed.

公开(公告)号：US07141650B2

公开(公告)日：2006-11-28

申请号：US10530886

申请日：2003-10-09

申请人： Tamaki Oda ,  Hideki Endoh ,  Yoshitaka Ueda ,  Kazunori Inabe

发明人： Tamaki Oda ,  Hideki Endoh ,  Yoshitaka Ueda ,  Kazunori Inabe

IPC分类号： A61K14/00

CPC分类号： C07K14/4705 ,  C07K14/47

摘要： A novel polypeptide which is useful in screening of an agent for improving insulin resistance and an agent for improving glucose metabolism, a polynucleotide encoding the polypeptide, an expression vector comprising the polynucleotide, and a cell transformed with the expression vector are disclosed. The polypeptide is a protein expressed in skeletal muscle. When the protein is overexpressed, incorporation of sugar into a cell is inhibited.A method for screening of an agent for improving insulin resistance and/or an agent for improving glucose metabolism in which the polypeptide is used, and a method for producing a pharmaceutical composition for insulin resistance and/or glucose metabolism improvement comprising a substance obtained according to the method for screening as an active ingredient are also disclosed.

摘要翻译： 公开了可用于筛选改善胰岛素抵抗的药剂和改善葡萄糖代谢的药剂的新型多肽，编码多肽的多核苷酸，包含多核苷酸的表达载体和用表达载体转化的细胞。 多肽是在骨骼肌中表达的蛋白质。 当蛋白质过表达时，将糖掺入细胞被抑制。 筛选用于改善胰岛素抵抗的药剂的方法和/或其中使用多肽的改善葡萄糖代谢的药剂，以及制备用于胰岛素抵抗和/或葡萄糖代谢改善的药物组合物的方法，包括根据 还公开了作为活性成分的筛选方法。

公开(公告)号：US20080171353A1

公开(公告)日：2008-07-17

申请号：US11834900

申请日：2007-08-07

申请人： Tomoyasu SUGIYAMA ,  Noriyuki Morikawa ,  Ai Wakamatsu ,  Tamaki Oda ,  Ryotaro Irie ,  Takao Isogai ,  Yasuhiko Masuho

发明人： Tomoyasu SUGIYAMA ,  Noriyuki Morikawa ,  Ai Wakamatsu ,  Tamaki Oda ,  Ryotaro Irie ,  Takao Isogai ,  Yasuhiko Masuho

IPC分类号： C12Q1/02

CPC分类号： C07K14/705 ,  G01N2333/4719 ,  G01N2500/00

摘要： The clone PLACE6002312 having a structure characteristic of G protein-coupled receptor was isolated from a human placental full-length cDNA library prepared by the oligo-capping method. Database search revealed that PLACE6002312 had the highest homology to HISTAMINE H2 RECEPTOR. Analysis for the expression of PLACE6002312 gene in human tissues revealed that the gene was expressed in various tissues, such as heart, liver, and ovary. In addition, histamine was found to be one of ligands for PLACE 6002312 by ligand-binding assay. Furthermore, a full-length cDNA for the mouse homolog of PLACE6002312 was isolated and the deduced amino acid sequence was revealed to comprise a structure characteristic of G protein-coupled receptor. PLACE6002312 can be used to screen for agonists and antagonists useful as pharmaceuticals and to diagnose various diseases caused by the abnormal activity or expression of the polypeptide.

摘要翻译： 从通过寡聚体封端法制备的人胎盘全长cDNA文库中分离出具有G蛋白偶联受体结构特征的克隆PLACE6002312。 数据库检索显示PLACE6002312与HISTAMINE H2受体的同源性最高。 对人类组织中PLACE6002312基因表达的分析显示，该基因在各种组织中表达，如心脏，肝脏和卵巢。 此外，通过配体结合测定，发现组胺是PLACE 6002312的配体之一。 此外，分离出PLACE6002312的小鼠同源物的全长cDNA，并推测出氨基酸序列包含G蛋白偶联受体的结构特征。 PLACE6002312可用于筛选可用作药物的激动剂和拮抗剂，并诊断由多肽的异常活性或表达引起的各种疾病。