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公开(公告)号：US09188594B2
公开(公告)日：2015-11-17
申请号：US12535396
申请日：2009-08-04
IPC分类号： G01N33/543 , G01N33/68
CPC分类号： G01N33/54366 , G01N33/54373 , G01N33/6863 , G01N33/6866 , G01N33/6869 , G01N2333/54 , G01N2333/555 , G01N2333/98
摘要： The present invention relates to a device and method for determining the presence of a specific compound in solution. The device includes a nanosensor having an electrically conducting pathway between at least a first and second contact. The device also includes a first receptor, suitable for binding a specific compound in the solution, attached to the nanosensor, and a second receptor also suitable for binding the specific compound while the specific compound is bound to the first receptor. The second receptor is attached to an enzyme added to the solution. When the solution having the second receptor is added to the device, and a second compound that is a substrate for the enzyme is subsequently added to the solution, a measured difference in an electrical property in the device before and after the application of the second compound is indicative of the presence of the specific compound in the solution.
摘要翻译： 本发明涉及一种用于测定溶液中特定化合物的存在的装置和方法。 该装置包括在至少第一和第二接触之间具有导电路径的纳米传感器。 该装置还包括适于结合附着于纳米传感器的溶液中的特定化合物的第一受体，以及也适于结合特定化合物而第二受体，而特异性化合物与第一受体结合。 第二受体与添加到溶液中的酶连接。 当将具有第二受体的溶液加入到装置中，并且随后将另外作为酶底物的第二化合物加入到溶液中时，测量第二化合物施用前后装置的电性能差异 表明该溶液中特定化合物的存在。
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公开(公告)号：US09599614B2
公开(公告)日：2017-03-21
申请号：US14005056
申请日：2012-03-14
申请人： Tarek M. Fahmy , Aleksandar Vacic , Mark A. Reed
发明人： Tarek M. Fahmy , Aleksandar Vacic , Mark A. Reed
IPC分类号： G01N27/414 , G01N33/543
CPC分类号： G01N33/54373 , G01N27/4145 , G01N27/4146 , G01N33/5438
摘要： The present invention relates to uniform nanostructure biosensors and methods of calibrating the response of nanostructure biosensors. The invention overcomes device to device variability that has made quantitative detection difficult. The described biosensors have uniform characteristics that allow for more reliable comparison across devices. The methods of the invention comprise normalizing the initial current rate, as measured by the nanostructure biosensor following the addition of an analyte, to device characteristics of the biosensor. The device characteristics of the biosensor which can be used to normalize the response include baseline current and transconductance. Calibration of responses allows for the generation of calibration curves for use in all devices to quantitatively detect an analyte, without the need for individual device calibration.
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公开(公告)号：US20100184104A1
公开(公告)日：2010-07-22
申请号：US12535396
申请日：2009-08-04
申请人： Tarek M. FAHMY , Eric D. Stern , Mark A. Reed , Aleksandar Vacic
发明人： Tarek M. FAHMY , Eric D. Stern , Mark A. Reed , Aleksandar Vacic
CPC分类号： G01N33/54366 , G01N33/54373 , G01N33/6863 , G01N33/6866 , G01N33/6869 , G01N2333/54 , G01N2333/555 , G01N2333/98
摘要： The present invention relates to a device and method for determining the presence of a specific compound in solution. The device includes a nanosensor having an electrically conducting pathway between at least a first and second contact. The device also includes a first receptor, suitable for binding a specific compound in the solution, attached to the nanosensor, and a second receptor also suitable for binding the specific compound while the specific compound is bound to the first receptor. The second receptor is attached to an enzyme added to the solution. When the solution having the second receptor is added to the device, and a second compound that is a substrate for the enzyme is subsequently added to the solution, a measured difference in an electrical property in the device before and after the application of the second compound is indicative of the presence of the specific compound in the solution.
摘要翻译： 本发明涉及一种用于测定溶液中特定化合物的存在的装置和方法。 该装置包括在至少第一和第二接触之间具有导电路径的纳米传感器。 该装置还包括适于结合附着于纳米传感器的溶液中的特定化合物的第一受体，以及也适于结合特定化合物而第二受体，而特异性化合物与第一受体结合。 第二受体与添加到溶液中的酶连接。 当将具有第二受体的溶液加入到装置中，并且随后将另外作为酶底物的第二化合物加入到溶液中时，测量第二化合物施用前后装置的电性能差异 表明该溶液中特定化合物的存在。
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