公开(公告)号：US5869300A

公开(公告)日：1999-02-09

申请号：US974919

申请日：1997-11-20

申请人： Tatsuya Yoshioka ,  Toshimasa Ishii ,  Yoshio Kawahara ,  Yosuke Koyama ,  Eiko Shimizu

发明人： Tatsuya Yoshioka ,  Toshimasa Ishii ,  Yoshio Kawahara ,  Yosuke Koyama ,  Eiko Shimizu

IPC分类号： C12P13/14 ,  C12R1/13 ,  C12P13/18

CPC分类号： C12P13/14 ,  Y10S435/813 ,  Y10S435/84 ,  Y10S435/843

摘要： A method for producing L-glutamic acid, comprising inoculating a microorganism having an ability to produce L-glutamic acid, in a liquid medium containing a carbon source and a nitrogen source, conducting continuous L-glutamic acid fermentation in which both a carbon source and a nutrient having an effect of promoting bacterial growth are fed so as to make the microorganism grow, and then collecting L-glutamic acid produced and accumulated in a culture broth.

摘要翻译： 一种生产L-谷氨酸的方法，包括在含有碳源和氮源的液体培养基中接种具有产生L-谷氨酸能力的微生物，进行连续的L-谷氨酸发酵，其中碳源和 喂养具有促进细菌生长的作用的营养物，使微生物生长，然后收集培养液中产生并积累的L-谷氨酸。

公开(公告)号：US5912113A

公开(公告)日：1999-06-15

申请号：US905713

申请日：1997-08-04

申请人： Takashi Nakamura ,  Tatsuya Nakayama ,  Yosuke Koyama ,  Keishi Shimazaki ,  Harufumi Miwa ,  Minoru Tsuruta ,  Koji Tamura ,  Osamu Tosaka

发明人： Takashi Nakamura ,  Tatsuya Nakayama ,  Yosuke Koyama ,  Keishi Shimazaki ,  Harufumi Miwa ,  Minoru Tsuruta ,  Koji Tamura ,  Osamu Tosaka

IPC分类号： C12M1/36 ,  C12N1/20 ,  C12P1/00 ,  C12P13/06 ,  C12P13/08 ,  C12Q3/00 ,  C12N1/12 ,  C12N1/14

CPC分类号： C12M41/32 ,  C12M41/26 ,  C12M41/34 ,  C12M41/48 ,  C12N1/20 ,  C12P13/08 ,  Y10S435/822 ,  Y10S435/948

摘要： The present invention is a method for aerobically cultivating yeast or bacteria in a culture medium of fed-batch, continuous or cell-recycling continuous cultures, wherein the carbon source concentration in the culture medium is maintained at a constant low level of under g/l. The carbon source concentration is maintained by measuring the carbon consumption of a culture of the yeast or bacteria in a preliminary experiment. The rate is determined between the time the culture is started and a time when the carbon source is exhausted. A feeding time is then determined wherein the activity of the yeast or bacteria in the presence of the carbon source does not change and a volume of the carbon source to be used in a first feeding (So) is set as So=.nu..times.T. Then, in a main culture, a first feeding of a volume of the carbon source (So) is added for the time (T), and the exhaustion of the carbon source is detected as an increase in pH or an increase in concentration of oxygen dissolved in the culture medium. A second feeding of a volume of the carbon source is then added for the time (T), and the feeding rate is determined based on a period (.tau.) before which the second feeding is added as follows:if 10 min..gtoreq..tau., the feeding rate of the feed solution is set at 1.1.times..nu.;if 30 min..gtoreq..tau.>10 min., the feeding rate of the feed solution is set at .nu.;if 60 min..gtoreq..tau.>30 min., the feeding rate is set at 0.9.times..nu.; orif 120 min..gtoreq..tau.>60 min., the feeding rate is set at 0.8.times..nu.. By modifying the feeding rate in this way, the carbon source concentration in the cultivation vessel is kept at the constant low level of under 5 g/l.

摘要翻译： 本发明是在补料分批，连续或细胞循环连续培养的培养基中有氧培养酵母或细菌的方法，其中培养基中的碳源浓度保持在低于g / l的恒定低水平 。 通过在初步实验中测量酵母或细菌的培养物的碳消耗来维持碳源浓度。 速率是在文化开始时间和碳源耗尽时间之间确定的。 然后确定进料时间，其中在碳源存在下酵母或细菌的活性不变，并且在第一次进料（So）中使用的碳源的体积被设定为So = nu xT。 然后，在主培养中，在时间（T）下加入体积的碳源（So）的第一次进料，并且随着pH的增加或氧浓度的增加检测碳源的耗尽 溶解在培养基中。 然后在时间（T）下加入体积的碳源的第二次进料，并且基于如下加入第二次进料的时间段（τ）确定进料速率：如果10分钟> / = tau，进料溶液的进料速率设定为1.1×nu; 如果30分钟，则进料溶液的进料速度设定为nu; 如果60分钟> 30分钟，进料速率设定为0.9x nu; 或者如果120分钟，则进料速率设定为0.8x nu。 通过以这种方式改变进料速率，培养容器中的碳源浓度保持在5g / l以下的恒定低水平。

公开(公告)号：US4529697A

公开(公告)日：1985-07-16

申请号：US475648

申请日：1983-03-15

申请人： Minoru Yoshimura ,  Yosuke Koyama ,  Koichi Goto ,  Sumio Inoue ,  Shigeho Ikeda ,  Hiroe Yoshii

发明人： Minoru Yoshimura ,  Yosuke Koyama ,  Koichi Goto ,  Sumio Inoue ,  Shigeho Ikeda ,  Hiroe Yoshii

IPC分类号： C12N9/02 ,  C12P13/14 ,  C12R1/13 ,  C12R1/15 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/0089 ,  C12P13/14 ,  Y10S435/84 ,  Y10S435/843

摘要： A process for producing L-glutamic acid by fermentation is disclosed, which process comprises culturing aerobically in a culture medium a mutant of the genus Brevibacterium or Corynebacterium which has an increased superoxide dismutase activity and is capable of producing L-glutamic acid in the culture medium and recovering the L-glutamic acid. The yield of L-glutamic acid can be increased by using the aforementioned mutants.

摘要翻译： 公开了通过发酵生产L-谷氨酸的方法，该方法包括在培养基中培养具有增加的超氧化物歧化酶活性并能够在培养基中产生L-谷氨酸的短杆菌属或棒状杆菌属的突变体 并回收L-谷氨酸。 通过使用上述突变体可以提高L-谷氨酸的产率。

公开(公告)号：US08741170B2

公开(公告)日：2014-06-03

申请号：US12572728

申请日：2009-10-02

申请人： Yasuhiko Fujii ,  Minoru Ohsugi ,  Yasushi Nishio ,  Yosuke Koyama ,  Shigeru Takaragi

发明人： Yasuhiko Fujii ,  Minoru Ohsugi ,  Yasushi Nishio ,  Yosuke Koyama ,  Shigeru Takaragi

IPC分类号： C04B35/26 ,  C04B35/64

CPC分类号： H01F1/01 ,  C01G49/0036 ,  C01P2002/52 ,  C01P2006/10 ,  C01P2006/12 ,  C01P2006/42 ,  C09C1/22 ,  H01F1/113 ,  H01F1/42 ,  H01F41/0273 ,  Y10T428/2982

摘要： The present invention relates to ferrite particles for bonded magnet, having a volume-average particle diameter of 2.1 to 2.7 μm and a particle diameter x90 of 4.3 to 5.4 μm wherein the x90 represents a particle diameter at which a cumulative percentage of particles under sieve (undersize particles) based on a volume thereof is 90%, when determined from a particle size distribution thereof measured by using a laser diffraction type particle size distribution measuring apparatus.

摘要翻译： 本发明涉及体积平均粒径为2.1〜2.7μm，粒径×90为4.3〜5.4μm的粘结磁体用铁氧体颗粒，其中，x90表示筛子下颗粒的累积百分数 当通过使用激光衍射型粒度分布测量装置测量的粒度分布确定时，基于其体积的小尺寸颗粒为90％。

公开(公告)号：US20100065771A1

公开(公告)日：2010-03-18

申请号：US12572728

申请日：2009-10-02

申请人： Yasuhiko FUJII ,  Minoru Ohsugi ,  Yasushi Nishio ,  Yosuke Koyama ,  Shigeru Takaragi

发明人： Yasuhiko FUJII ,  Minoru Ohsugi ,  Yasushi Nishio ,  Yosuke Koyama ,  Shigeru Takaragi

IPC分类号： H01F1/26 ,  B32B5/16

CPC分类号： H01F1/01 ,  C01G49/0036 ,  C01P2002/52 ,  C01P2006/10 ,  C01P2006/12 ,  C01P2006/42 ,  C09C1/22 ,  H01F1/113 ,  H01F1/42 ,  H01F41/0273 ,  Y10T428/2982

摘要： The present invention relates to ferrite particles for bonded magnet, having a volume-average particle diameter of 2.1 to 2.7 μm and a particle diameter x90 of 4.3 to 5.4 μm wherein the x90 represents a particle diameter at which a cumulative percentage of particles under sieve (undersize particles) based on a volume thereof is 90%, when determined from a particle size distribution thereof measured by using a laser diffraction type particle size distribution measuring apparatus.

摘要翻译： 本发明涉及体积平均粒径为2.1〜2.7μm，粒径×90为4.3〜5.4μm的粘结磁体用铁氧体颗粒，其中，x90表示筛子下颗粒的累积百分数 当通过使用激光衍射型粒度分布测量装置测量的粒度分布确定时，基于其体积的小尺寸颗粒为90％。

公开(公告)号：US6025169A

公开(公告)日：2000-02-15

申请号：US192565

申请日：1998-11-17

申请人： Takashi Nakamura ,  Tatsuya Nakayama ,  Yosuke Koyama ,  Keishi Shimazaki ,  Harufumi Miwa ,  Minoru Tsuruta ,  Koji Tamura ,  Osamu Tosaka

发明人： Takashi Nakamura ,  Tatsuya Nakayama ,  Yosuke Koyama ,  Keishi Shimazaki ,  Harufumi Miwa ,  Minoru Tsuruta ,  Koji Tamura ,  Osamu Tosaka

IPC分类号： C12M1/36 ,  C12N1/20 ,  C12P1/00 ,  C12P13/06 ,  C12P13/08

CPC分类号： C12M41/32 ,  C12M41/26 ,  C12M41/34 ,  C12M41/48 ,  C12N1/20 ,  C12P13/08 ,  Y10S435/822 ,  Y10S435/948

摘要： The present invention relates to a method for controlling carbon source concentration in the aerobic cultivation of a microorganism. The substrate carbon source remains at a low level in a cultivation vessel during the culture feeding in aerobic fed-batch, continuous or cell-recycling continuous cultures. This is accomplished by monitoring the increase in pH or dissolved oxygen content in the culture medium and adding the feed solution intermittently into a cultivation vessel at a calculated feed rate using a feed control device controlled by a computer.The present invention further relates to a process for producing L-lysine by fermentation having the advantages over the prior methods, those being improved productivity, higher concentrations of accumulated product, and increased yields of L-lysine.

摘要翻译： 本发明涉及一种控制微生物需氧培养中碳源浓度的方法。 在需氧补料分批，连续或细胞循环连续培养的培养进料期间，底物碳源在培养容器中保持在低水平。 这是通过监测培养基中pH或溶解氧含量的增加并使用由计算机控制的进料控制装置以计算的进料速率间歇地将进料溶液加入到培养容器中来实现的。 本发明还涉及通过发酵生产L-赖氨酸的方法，其具有优于现有方法的优点，即提高生产率，较高浓度的积累产物和增加的L-赖氨酸的产率。