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公开(公告)号：US11629332B2
公开(公告)日：2023-04-18
申请号：US15943619
申请日：2018-04-02
申请人： Terumo BCT, Inc.
发明人： Mark E. Jones , Brian J. Nankervis
IPC分类号： C12N5/0783 , C12M1/00 , C12M1/12 , C12M1/36 , C12M1/42
摘要： Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.
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公开(公告)号：US09617506B2
公开(公告)日：2017-04-11
申请号：US14542276
申请日：2014-11-14
申请人： Terumo BCT, Inc.
发明人： Mark E. Jones , Nathan D. Frank
CPC分类号： C12M29/18 , C12M23/50 , C12M25/10 , C12M25/12 , C12M27/10 , C12M29/10 , C12M29/16 , C12M41/48 , C12N5/0663
摘要： Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.
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3.发明申请公开(公告)号：US20140051167A1
公开(公告)日：2014-02-20
申请号：US13971652
申请日：2013-08-20
申请人： Terumo BCT, Inc.
IPC分类号： C12M1/00
摘要： One or more embodiments are described directed to a method and system for concentrating components of a fluid circulated through a cell growth chamber such as a cell growth chamber. Accordingly, embodiments include methods and systems that utilize a tangential flow filter to concentrate components of a fluid that in embodiments includes expanded cells. In embodiments, a concentrated fluid component and a concentrated cell component are generated by flowing the fluid with expanded cells across a tangential flow filter. The concentrated cell component may be recirculated to the tangential flow filter to reach some desired concentration of cells. The concentrated fluid component may be collected to utilize cellular-produced constituents in the concentrated fluid component.
摘要翻译： 对一个或多个实施方案进行描述，涉及用于浓缩循环通过诸如细胞生长室的细胞生长室的流体的组分的方法和系统。 因此，实施方案包括使用切向流过滤器来浓缩流体的组分的方法和系统，其在实施方案中包括扩展的细胞。 在实施方案中，通过使具有膨胀的细胞的流体流过切向流过滤器来产生浓缩的流体组分和浓缩的细胞组分。 浓缩的细胞组分可以再循环到切向流过滤器以达到某些所需浓度的细胞。 可以收集浓缩流体组分以利用浓缩流体组分中的细胞产生的成分。
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公开(公告)号：US20220306978A1
公开(公告)日：2022-09-29
申请号：US17702658
申请日：2022-03-23
申请人： Terumo BCT, Inc.
发明人： Mark E. Jones , Dalip Sethi , Dennis J. Hlavinka , Thomas J. Felt
摘要： Implementations are described that relate to methods and systems for growing cells in a hollow fiber bioreactor. In implementations, the cells may be exposed to a number of growth factors including a combination of recombinant growth factors. In other implementations, the cells may be grown in co-culture with other cells, e.g., hMSC's. In implementations, the cells may include CD34+ cells. A coated membrane includes a membrane having a first coating configured to promote cellular adhesion to the membrane and a second coating that includes a soluble protein moiety.
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公开(公告)号：US10557112B2
公开(公告)日：2020-02-11
申请号：US15482168
申请日：2017-04-07
申请人： Terumo BCT, Inc.
发明人： Nathan D. Frank , Mark E. Jones
摘要： Described are embodiments for expanding cells in a bioreactor. In embodiments, methods and systems are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.
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公开(公告)号：US11624046B2
公开(公告)日：2023-04-11
申请号：US16112498
申请日：2018-08-24
申请人： Terumo BCT, Inc.
摘要： Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), helper, naïve, memory, or effector, for example.
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公开(公告)号：US11608486B2
公开(公告)日：2023-03-21
申请号：US15852689
申请日：2017-12-22
申请人： Terumo BCT, Inc.
摘要： Embodiments for loading and expanding particular cell types are described. Embodiments may include the use of hollow fiber membranes with particular characteristic such as hollow fibers with inner diameters that provide mechanical stimulus (e.g., radius of curvature greater than a dimension of a cell). In addition, embodiments may provide for manipulation of flow rates and other features that also provide mechanical stimuli and promote or enhance the growth of particular types of cells.
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公开(公告)号：US09695393B2
公开(公告)日：2017-07-04
申请号：US13971652
申请日：2013-08-20
申请人： Terumo BCT, Inc.
摘要： One or more embodiments are described directed to a method and system for concentrating components of a fluid circulated through a cell growth chamber such as a cell growth chamber. Accordingly, embodiments include methods and systems that utilize a tangential flow filter to concentrate components of a fluid that in embodiments includes expanded cells. In embodiments, a concentrated fluid component and a concentrated cell component are generated by flowing the fluid with expanded cells across a tangential flow filter. The concentrated cell component may be recirculated to the tangential flow filter to reach some desired concentration of cells. The concentrated fluid component may be collected to utilize cellular-produced constituents in the concentrated fluid component.
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