公开(公告)号：US20050158725A1

公开(公告)日：2005-07-21

申请号：US10510819

申请日：2003-09-24

申请人： Tetsuo Yukimasa ,  Nobuhiko Ozaki ,  Masaya Nakatani ,  Hidenobu Yaku ,  Hiroaki Oka ,  Kimi Fukahi

发明人： Tetsuo Yukimasa ,  Nobuhiko Ozaki ,  Masaya Nakatani ,  Hidenobu Yaku ,  Hiroaki Oka ,  Kimi Fukahi

IPC分类号： C07H21/00 ,  C12Q1/68 ,  C12M1/34

CPC分类号： C07H21/00 ,  C12Q1/6844

摘要： The present invention provides a reaction container, a reaction device, and a method of amplifying a nucleic acid, which are suitable for locally heating a sample which contains a nucleic acid, enable rapid control of temperature changes in the sample, provide easy fabrication, and are low cost. The invention provides a reaction container used for a nucleic acid amplification reaction, which includes a sample holding portion formed from a cavity in the reaction container; and a heating element for heating a sample which contains a nucleic acid and which is held in the sample holding portion, wherein the heating element is made of a conductive material and arranged at a location where the sample or the sample and the periphery of the cavity is (are) to be locally heated, the conductive material generating heat by electromagnetic induction.

摘要翻译： 本发明提供一种反应容器，反应装置和扩增核酸的方法，其适用于局部加热含有核酸的样品，能够快速控制样品中的温度变化，提供容易的制造，以及 是低成本的。 本发明提供了用于核酸扩增反应的反应容器，其包括由反应容器中的空腔形成的样品保持部分; 以及加热元件，用于加热含有核酸并被保持在样品保持部分中的样品，其中所述加热元件由导电材料制成并且布置在样品或样品和空腔周边的位置 被局部加热，导电材料通过电磁感应产生热量。

公开(公告)号：US07267986B2

公开(公告)日：2007-09-11

申请号：US10699848

申请日：2003-11-04

申请人： Tetsuo Yukimasa ,  Hidenobu Yaku ,  Kimi Fukahi

发明人： Tetsuo Yukimasa ,  Hidenobu Yaku ,  Kimi Fukahi

IPC分类号： G01N33/00

CPC分类号： C12Q1/6827 ,  C12Q1/6858 ,  G01N33/84 ,  C12Q2535/125 ,  C12Q2565/301

摘要： According to the present invention, an inorganic phosphoric acid is detected by a method which includes: subjecting a sample to a measurement system containing glyceraldehyde-3-phosphate, oxidized nicotinamide adenine dinucleotide or oxidized nicotinamide adenine dinucleotide phosphate, glyceraldehyde phosphate dehydrogenase, and an electron mediator; and measuring a current value in the measurement system. In the method, a pyrophosphate is quantitatively measured with high sensitivity and at a high speed through converting the pyrophosphate in a sample into an inorganic phosphoric acid. Such a measurement of a pyrophosphate allows for quantitative determination of the pyrophosphate which is produced concurrent with the extension of a DNA, thereby enabling the detection of the presence of a targeted nucleic acid, and typing of a base in a SNP site of a targeted DNA.

摘要翻译： 根据本发明，通过以下方法检测无机磷酸：将样品置于含有甘油醛-3-磷酸酯，氧化烟酰胺腺嘌呤二核苷酸或氧化烟酰胺腺嘌呤二核苷酸磷酸酯的测定体系，甘油醛磷酸脱氢酶，电子 调解员 并测量测量系统中的当前值。 在该方法中，通过将样品中的焦磷酸盐转化为无机磷酸，以高灵敏度和高速定量测定焦磷酸盐。 焦磷酸盐的这种测量允许定量测定与DNA延伸同时产生的焦磷酸盐，从而能够检测目标核酸的存在，以及靶向DNA的SNP位点中碱基的分型 。