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    Method of amplifying nucleic acid by electromagnetic induction heating and reaction container and reaction device to be used therein
    1.
    发明申请
    Method of amplifying nucleic acid by electromagnetic induction heating and reaction container and reaction device to be used therein 审中-公开
    通过电磁感应加热和反应容器放大核酸的方法及其中使用的反应装置

    公开(公告)号：US20050158725A1

    公开(公告)日：2005-07-21

    申请号：US10510819

    申请日：2003-09-24

    申请人： Tetsuo Yukimasa ,  Nobuhiko Ozaki ,  Masaya Nakatani ,  Hidenobu Yaku ,  Hiroaki Oka ,  Kimi Fukahi

    发明人： Tetsuo Yukimasa ,  Nobuhiko Ozaki ,  Masaya Nakatani ,  Hidenobu Yaku ,  Hiroaki Oka ,  Kimi Fukahi

    IPC分类号： C07H21/00 ,  C12Q1/68 ,  C12M1/34

    CPC分类号： C07H21/00 ,  C12Q1/6844

    摘要： The present invention provides a reaction container, a reaction device, and a method of amplifying a nucleic acid, which are suitable for locally heating a sample which contains a nucleic acid, enable rapid control of temperature changes in the sample, provide easy fabrication, and are low cost. The invention provides a reaction container used for a nucleic acid amplification reaction, which includes a sample holding portion formed from a cavity in the reaction container; and a heating element for heating a sample which contains a nucleic acid and which is held in the sample holding portion, wherein the heating element is made of a conductive material and arranged at a location where the sample or the sample and the periphery of the cavity is (are) to be locally heated, the conductive material generating heat by electromagnetic induction.

    摘要翻译： 本发明提供一种反应容器，反应装置和扩增核酸的方法，其适用于局部加热含有核酸的样品，能够快速控制样品中的温度变化，提供容易的制造，以及 是低成本的。 本发明提供了用于核酸扩增反应的反应容器，其包括由反应容器中的空腔形成的样品保持部分; 以及加热元件，用于加热含有核酸并被保持在样品保持部分中的样品，其中所述加热元件由导电材料制成并且布置在样品或样品和空腔周边的位置 被局部加热，导电材料通过电磁感应产生热量。

    Blood processing apparatus and blood introducing method
    3.
    发明授权
    Blood processing apparatus and blood introducing method 有权
    血液处理装置及血液导入方法

    公开(公告)号：US07368083B2

    公开(公告)日：2008-05-06

    申请号：US11494749

    申请日：2006-07-28

    申请人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Tetsuo Yukimasa ,  Hidenobu Yaku ,  Maki Yotsuhashi ,  Yukari Hataoka

    发明人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Tetsuo Yukimasa ,  Hidenobu Yaku ,  Maki Yotsuhashi ,  Yukari Hataoka

    IPC分类号： G01N33/00

    CPC分类号： G01N35/00069 ,  Y10T436/111666 ,  Y10T436/2575

    摘要： The present invention provides a blood processing apparatus that can introduce a metered quantity of a blood sample into a chamber and does not require an opening linked to the chamber to be closed after the introduction of the blood sample. An introducing chamber, an injection port, and an air port are formed in a rotatable platform of a blood processing apparatus. The injection port links the introducing chamber with the outside of the rotatable platform. A blood sample is introduced from the injection port into the introducing chamber. A blood coagulant is held on the port wall surface of the air port and/or around a portion of the surface of the rotatable platform where the air port is open.

    摘要翻译： 本发明提供一种血液处理装置，其能够将计量量的血液样品引入腔室中，并且在引入血液样本之后不需要连接到待关闭的腔室的开口。 在血液处理装置的可旋转平台中形成有引入室，注射口和空气口。 注入口将引入室与可旋转平台的外部连接。 血液样品从注射口引入引入室。 血液凝结剂被保持在空气端口的端口壁表面上和/或在空气端口打开的可旋转平台的表面的一部分周围。

    Blood processing apparatus and blood introducing method
    5.
    发明申请
    Blood processing apparatus and blood introducing method 有权
    血液处理装置及血液导入方法

    公开(公告)号：US20060263247A1

    公开(公告)日：2006-11-23

    申请号：US11494749

    申请日：2006-07-28

    申请人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Tetsuo Yukimasa ,  Hidenobu Yaku ,  Maki Yotsuhashi ,  Yukari Hataoka

    发明人： Nobuhiko Ozaki ,  Hiroaki Oka ,  Tetsuo Yukimasa ,  Hidenobu Yaku ,  Maki Yotsuhashi ,  Yukari Hataoka

    IPC分类号： G01N33/00

    CPC分类号： G01N35/00069 ,  Y10T436/111666 ,  Y10T436/2575

    摘要： The present invention provides a blood processing apparatus that can introduce a metered quantity of a blood sample into a chamber and does not require an opening linked to the chamber to be closed after the introduction of the blood sample. An introducing chamber, an injection port, and an air port are formed in a rotatable platform of a blood processing apparatus. The injection port links the introducing chamber with the outside of the rotatable platform. A blood sample is introduced from the injection port into the introducing chamber. A blood coagulant is held on the port wall surface of the air port and/or around a portion of the surface of the rotatable platform where the air port is open.

    摘要翻译： 本发明提供一种血液处理装置，其能够将计量量的血液样品引入腔室中，并且在引入血液样本之后不需要连接到待关闭的腔室的开口。 在血液处理装置的可旋转平台中形成有引入室，注射口和空气口。 注入口将引入室与可旋转平台的外部连接。 血液样品从注射口引入引入室。 血液凝结剂被保持在空气端口的端口壁表面上和/或在空气端口打开的可旋转平台的表面的一部分周围。

    Method of detecting inorganic phosphoric acid, pyrophosphate and nucleic acid, and method of typing SNP sequence of DNA
    6.
    发明授权
    Method of detecting inorganic phosphoric acid, pyrophosphate and nucleic acid, and method of typing SNP sequence of DNA 有权
    检测无机磷酸，焦磷酸盐和核酸的方法，以及分型DNA的SNP序列的方法

    公开(公告)号：US07267986B2

    公开(公告)日：2007-09-11

    申请号：US10699848

    申请日：2003-11-04

    申请人： Tetsuo Yukimasa ,  Hidenobu Yaku ,  Kimi Fukahi

    发明人： Tetsuo Yukimasa ,  Hidenobu Yaku ,  Kimi Fukahi

    IPC分类号： G01N33/00

    CPC分类号： C12Q1/6827 ,  C12Q1/6858 ,  G01N33/84 ,  C12Q2535/125 ,  C12Q2565/301

    摘要： According to the present invention, an inorganic phosphoric acid is detected by a method which includes: subjecting a sample to a measurement system containing glyceraldehyde-3-phosphate, oxidized nicotinamide adenine dinucleotide or oxidized nicotinamide adenine dinucleotide phosphate, glyceraldehyde phosphate dehydrogenase, and an electron mediator; and measuring a current value in the measurement system. In the method, a pyrophosphate is quantitatively measured with high sensitivity and at a high speed through converting the pyrophosphate in a sample into an inorganic phosphoric acid. Such a measurement of a pyrophosphate allows for quantitative determination of the pyrophosphate which is produced concurrent with the extension of a DNA, thereby enabling the detection of the presence of a targeted nucleic acid, and typing of a base in a SNP site of a targeted DNA.

    摘要翻译： 根据本发明，通过以下方法检测无机磷酸：将样品置于含有甘油醛-3-磷酸酯，氧化烟酰胺腺嘌呤二核苷酸或氧化烟酰胺腺嘌呤二核苷酸磷酸酯的测定体系，甘油醛磷酸脱氢酶，电子 调解员 并测量测量系统中的当前值。 在该方法中，通过将样品中的焦磷酸盐转化为无机磷酸，以高灵敏度和高速定量测定焦磷酸盐。 焦磷酸盐的这种测量允许定量测定与DNA延伸同时产生的焦磷酸盐，从而能够检测目标核酸的存在，以及靶向DNA的SNP位点中碱基的分型 。

    Discrimination method of target base in DNA, and allele specific primer used in the method of the same
    7.
    发明授权
    Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
    DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物

    公开(公告)号：US07396648B2

    公开(公告)日：2008-07-08

    申请号：US11607907

    申请日：2006-12-04

    申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6827 ,  Y10T436/143333

    摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is G. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of T or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

    摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基为G时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 一个基座间隔开的是G.根据本发明，3'端基被设计为对应于SNP的基极; 从3'端的第二个基地是T或G; 从3'的第三个基地是T或G的任何一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

    Discrimination method of target base in DNA, and allele specific primer used in the method of the same
    9.
    发明申请
    Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
    DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物

    公开(公告)号：US20070281341A1

    公开(公告)日：2007-12-06

    申请号：US11633584

    申请日：2006-12-05

    申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/6858 ,  C12Q1/6869 ,  C12Q2535/125

    摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is C.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of A, T or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

    摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基为G时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 其中一个基座间隔开C.根据本发明，3'端基被设计为对应于SNP的基底; 从3'端的第二个基地是T或G; 3'端的第3个碱基为A，T或C之一; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

    Discrimination method of target base in DNA, and allele specific primer used in the method of the same
    10.
    发明授权
    Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
    DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物

    公开(公告)号：US07297498B2

    公开(公告)日：2007-11-20

    申请号：US11488635

    申请日：2006-07-19

    申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6858 ,  C12Q2535/125 ,  C12Q2525/185

    摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is C.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second and the third bases from the 3′ end to be 5′-AT-3′, 5′-TA-3′, 5′-TT-3′ or 5′-CT-3′; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

    摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基是C时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 其中一个基座间隔开C.根据本发明，3'端基被设计为对应于SNP的基底; 3'末端的第2和第3个碱基为5'-AT-3'，5'-TA-3'，5'-TT-3'或5'-CT-3' 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。