-
公开(公告)号：US11999966B2
公开(公告)日：2024-06-04
申请号：US17081983
申请日：2020-10-27
Inventor： Karl Martin Hjort , Sergio Leal-Ortiz , Yuhong Cao , Chris Rehse , Andy Kah Ping Tay , Nicholas A. Melosh
CPC classification number: C12N15/87 , C12M23/02 , C12M23/42 , C12M35/00 , C12M35/02 , C12M35/04 , B82Y5/00 , B82Y40/00 , C12M23/16 , C12N5/0068 , C12N5/0075 , C12N2521/00 , C12N2535/00
Abstract: Nanostraws and to methods of utilizing them in order to deliver biologically relevant molecules such as DNA, RNA, proteins etc., into non-adherent cells such as immune cells, embryos, plant cells, bacteria, yeast etc. The methods described herein are repeatedly capable of delivering biologically relevant cargo into non-adherent cells, with high cell viability, dosage control, unaffected proliferation or cellular development, and with high efficiency. Among other uses, these new delivery methods will allow to scale pre-clinical cell reprogramming techniques to clinical applications.
-
公开(公告)号：US20190125668A1
公开(公告)日：2019-05-02
申请号：US16083758
申请日：2017-03-14
Applicant: The Regents of the University of California , The Board of Trustees of the Leland Stanford Junior University
Inventor： Cade B. Fox , Hariharasudhan Chirra Dinakar , Nicholas A. Melosh , Tejal A. Desai
Abstract: Microdevices containing a chamber bound on one side by a nanoporous membrane are provided. The nanoporous membrane may contain hollow nanotubes that extend through the nanoporous membrane, from one surface to the other, and extend beyond the surface of the nanoporous membrane opposite the surface interfacing with the chamber. The nanotubes may provide a fluidic conduit between an environment external to the microdevice and the chamber, which is otherwise substantially fluid-tight. Also provided are methods of making a microdevice and methods of using the microdevices.
-
公开(公告)号：US10815499B2
公开(公告)日：2020-10-27
申请号：US16038062
申请日：2018-07-17
Inventor： Karl Martin Hjort , Sergio Leal-Ortiz , Yuhong Cao , Chris Rehse , Andy Kah Ping Tay , Nicholas A. Melosh
Abstract: Nanostraws and to methods of utilizing them in order to deliver biologically relevant molecules such as DNA, RNA, proteins etc., into non-adherent cells such as immune cells, embryos, plant cells, bacteria, yeast etc. The methods described herein are repeatedly capable of delivering biologically relevant cargo into non-adherent cells, with high cell viability, dosage control, unaffected proliferation or cellular development, and with high efficiency. Among other uses, these new delivery methods will allow to scale pre-clinical cell reprogramming techniques to clinical applications.
-
公开(公告)号：US20220093357A1
公开(公告)日：2022-03-24
申请号：US17538280
申请日：2021-11-30
Inventor： Daniel Riley , Kunal Sahasrabuddhe , Igor Bargatin , Jared Schwede , Zhixun Shen , Nicholas A. Melosh
Abstract: The embodiments provide a thermionic emission device and a method for tuning a work function in a thermionic emission device is provided. The method includes illuminating an N type semiconductor material of a first member of a thermionic emission device, wherein a work function of the N type semiconductor material is lowered by the illuminating. The method includes collecting, on one of the first member or a second member of the thermionic emission device, electrons emitted from one of the first member or the second member.
-
公开(公告)号：US11020345B2
公开(公告)日：2021-06-01
申请号：US16083758
申请日：2017-03-14
Applicant: The Regents of the University of California , The Board of Trustees of the Leland Stanford Junior University
Inventor： Cade B. Fox , Hariharasudhan Chirra Dinakar , Nicholas A. Melosh , Tejal A. Desai
Abstract: Microdevices containing a chamber bound on one side by a nanoporous membrane are provided. The nanoporous membrane may contain hollow nanotubes that extend through the nanoporous membrane, from one surface to the other, and extend beyond the surface of the nanoporous membrane opposite the surface interfacing with the chamber. The nanotubes may provide a fluidic conduit between an environment external to the microdevice and the chamber, which is otherwise substantially fluid-tight. Also provided are methods of making a microdevice and methods of using the microdevices.
-
Patent Agency Ranking
公开(公告)号：US20200376245A1
公开(公告)日：2020-12-03
申请号：US16891652
申请日：2020-06-03
Inventor： Abdulmalik M Obaid , Sergio Leal-Ortiz , Nicholas A. Melosh
Abstract: A high-throughput method, device and system technology is provided capable of unconstrained penetration into virtually any cell type. This technology is completely agnostic to the cargo type or size (DNA, RNA or protein), is ultra-robust due to use of stiff metals, and has a direct path to scalabillity. This technology will serve as an effective method of intracellular delivery. In addition, this device is reusable and capable with working with all cell types, regardless of cell stiffness, and is potentially capable of penetrating into the nucleus of a cell. An intra-cellularly delivery device with an elongated structure with an ultra-sharp tip of less than 10 nanometers enables this technology whereby intracellular access is gained with little to no observable deformation of the cell walls. This dramatically increases the likelihood of cell survival and successful delivery.
-
公开(公告)号：US11685897B2
公开(公告)日：2023-06-27
申请号：US16215503
申请日：2018-12-10
Inventor： Jules J. Vandersarl , Alexander M. Xu , Nicholas A. Melosh , Noureddine Tayebi
CPC classification number: C12N5/0075 , B82Y5/00 , B82Y40/00 , C12N5/0068 , C12N2521/00 , C12N2533/10 , C12N2533/30 , C12N2535/00
Abstract: In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to methods of making a structure including nanotubes, a structure including nanotubes, methods of delivering a fluid to a cell, methods of removing a fluid to a cell, methods of accessing intracellular space, and the like.
-
公开(公告)号：US11530378B2
公开(公告)日：2022-12-20
申请号：US16302364
申请日：2017-06-09
Inventor： Ryan T. Swoboda , Yuhong Cao , Sergio Leal-Ortiz , Stefanie Rothkoetter , Nicholas A. Melosh
Abstract: Described herein are nanostraw well insert apparatuses (e.g., devices and systems) that include nanotubes extending through and out of a membrane so that a material can pass through the membrane from a fluid reservoir depot and into a cell grown onto the nanotubes when electrical energy (e.g., electroporation energy) is applied. In particular, the device, systems and methods described herein may be adapted for cell growth viability and transfection efficiency (e.g., >70%). These apparatuses may be readily integratable into cell culturing processes for improved transfection efficiency, intracellular transport, and cell viability.
-
公开(公告)号：US11205554B1
公开(公告)日：2021-12-21
申请号：US14333431
申请日：2014-07-16
Inventor： Daniel Riley , Kunal Sahasrabuddhe , Igor Bargatin , Jared W. Schwede , Zhi-Xun Shen , Nicholas A. Melosh
Abstract: The embodiments provide a thermionic emission device and a method for tuning a work function in a thermionic emission device is provided. The method includes illuminating an N type semiconductor material of a first member of a thermionic emission device, wherein a work function of the N type semiconductor material is lowered by the illuminating. The method includes collecting, on one of the first member or a second member of the thermionic emission device, electrons emitted from one of the first member or the second member.
-
10.发明授权公开(公告)号：US11149266B2
公开(公告)日：2021-10-19
申请号：US16332684
申请日：2017-09-13
Inventor： Nicholas A. Melosh , Yuhong Cao , Karl Martin Hjort , Amanda Jonsson
IPC: C12M1/26 , C12N15/10 , C12M1/42 , C12M1/34 , C12N13/00 , C12Q1/24 , B82Y5/00 , B82Y15/00 , B82Y40/00
Abstract: Methods and apparatuses to non-destructively and periodically sample a small quantity of intracellular proteins and mRNA from the same single cell or cells for an extended period of time. Specifically, describe herein are non-perturbative methods for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and nucleic acids from a variety of cell types using systems including nanostraws.
-
-
-
-
-
-
-
-
-
Search Results
11
records
(took 0.047 seconds)
