公开(公告)号：US11634448B2

公开(公告)日：2023-04-25

申请号：US15624399

申请日：2017-06-15

申请人： The General Hospital Corporation

发明人： Harald C. Ott ,  Xi Ren ,  Jordan P. Bloom ,  Taufiek Konrad Rajab

IPC分类号： A61K31/655 ,  C12N5/06 ,  C07H13/04 ,  A61L27/38 ,  A61L27/54 ,  C12N5/07 ,  A61K35/22 ,  A61K35/34 ,  A61K35/36 ,  A61K35/407 ,  A61K35/42 ,  A61K35/44 ,  A61L27/36

摘要： The present application provides methods of functionalizing an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal or by culturing an organ or tissue in a bioreactor containing such nutrient. The present application also provides methods of selectively functionalizing extracellular matrix (ECM) of an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal. In some aspects, the present application provides a decellularized scaffold of a mammalian organ or tissue comprising an extracellular matrix, wherein the extracellular matrix of the decellularized scaffold is functionalized with a chemical group that is reactive in a bioorthogonal chemical reaction, such as an azide chemical group. The present application also provides biological prosthetic mesh and mammalian organs and tissues for transplantation prepared according to the methods of the application.

公开(公告)号：US20230287030A1

公开(公告)日：2023-09-14

申请号：US18118909

申请日：2023-03-08

申请人： The General Hospital Corporation

发明人： Harald C. Ott ,  Xi Ren ,  Jordan P. Bloom ,  Taufiek Konrad Rajab

IPC分类号： C07H13/04 ,  A61L27/38 ,  A61L27/54 ,  C12N5/07 ,  A61K31/655 ,  A61K35/22 ,  A61K35/34 ,  A61K35/36 ,  A61K35/407 ,  A61K35/42 ,  A61K35/44 ,  A61L27/36

CPC分类号： C07H13/04 ,  A61L27/3813 ,  A61L27/3826 ,  A61L27/383 ,  A61L27/3808 ,  A61L27/54 ,  C12N5/06 ,  A61K31/655 ,  A61K35/22 ,  A61K35/34 ,  A61K35/36 ,  A61K35/407 ,  A61K35/42 ,  A61K35/44 ,  A61L27/3633 ,  A61L2300/414 ,  A61L2300/256 ,  A61L2300/42 ,  A61L2300/406

摘要： The present application provides methods of functionalizing an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal or by culturing an organ or tissue in a bioreactor containing such nutrient. The present application also provides methods of selectively functionalizing extracellular matrix (ECM) of an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal. In some aspects, the present application provides a decellularized scaffold of a mammalian organ or tissue comprising an extracellular matrix, wherein the extracellular matrix of the decellularized scaffold is functionalized with a chemical group that is reactive in a bioorthogonal chemical reaction, such as an azide chemical group. The present application also provides biological prosthetic mesh and mammalian organs and tissues for transplantation prepared according to the methods of the application.