公开(公告)号：US20180071399A1

公开(公告)日：2018-03-15

申请号：US15694114

申请日：2017-09-01

申请人： The Government of the United States of America, as represented by the Secretary of the Navy

发明人： Igor L. Medintz ,  James B. Delehanty ,  Joyce Breger ,  Markus Muttenthaler ,  Philip E. Dawson

IPC分类号： A61K47/64 ,  A61K47/69 ,  A61K47/52 ,  A61K31/704

CPC分类号： A61K47/641 ,  A61K31/704 ,  A61K38/08 ,  A61K47/52 ,  A61K47/62 ,  A61K47/645 ,  A61K47/6923 ,  A61K47/6929

摘要： Nanoparticles (and optionally a cargo such as a drug) can be delivered to cells by attaching just a single dendritic peptide to the nanoparticle. The dendritic peptide includes a polyhisitidine motif and a hinge and a spacer connecting the polyhistidine to a lysine-based dendritic wedge displaying at least two copies of a cell-penetrating peptide motif.

公开(公告)号：US10705092B2

公开(公告)日：2020-07-07

申请号：US15882259

申请日：2018-01-29

申请人： The Government of the United States of America, as represented by the Secretary of the Navy

发明人： James B. Delehanty ,  Michael H. Stewart ,  Okhil Nag ,  Jeffrey R. Deschamps ,  Kimihiro Susumu ,  Eunkeu Oh ,  Lauren D. Field ,  Alexander L. Efros ,  Alan L. Huston ,  Igor L. Medintz ,  Philip E. Dawson

IPC分类号： G01N33/58

摘要： A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be C60 fullerene. Emission correlates with cellular membrane potential.

公开(公告)号：US20180326097A1

公开(公告)日：2018-11-15

申请号：US16031094

申请日：2018-07-10

申请人： The Government of the United States of America, as represented by the Secretary of the Navy

发明人： James B. Delehanty ,  Michael H. Stewart ,  Okhil Nag ,  Jeffrey R. Deschamps ,  Kimihiro Susumu ,  Eunkeu Oh ,  Lauren D. Field ,  Alexander L. Efros ,  Alan L. Huston ,  Igor L. Medintz ,  Philip E. Dawson ,  Nashaat Rasheed ,  Parag V. Chitnis ,  John R. Cressman

IPC分类号： A61K49/22 ,  G01N33/58

CPC分类号： A61K49/222 ,  A61K49/221 ,  G01N33/542 ,  G01N33/588

摘要： A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be C60 fullerene. Photoacoustic emission from the construct correlates with cellular membrane potential.

公开(公告)号：US10183080B2

公开(公告)日：2019-01-22

申请号：US15694114

申请日：2017-09-01

申请人： The Government of the United States of America, as represented by the Secretary of the Navy

发明人： Igor L. Medintz ,  James B. Delehanty ,  Joyce Breger ,  Markus Muttenthaler ,  Philip E. Dawson

IPC分类号： A61K38/08 ,  A61K47/64 ,  A61K47/69 ,  A61K31/704 ,  A61K47/52 ,  A61K47/62

摘要： Nanoparticles (and optionally a cargo such as a drug) can be delivered to cells by attaching just a single dendritic peptide to the nanoparticle. The dendritic peptide includes a polyhisitidine motif and a hinge and a spacer connecting the polyhistidine to a lysine-based dendritic wedge displaying at least two copies of a cell-penetrating peptide motif.

公开(公告)号：US11287430B2

公开(公告)日：2022-03-29

申请号：US16894920

申请日：2020-06-08

申请人： The Government of the United States of America, as represented by the Secretary of the Navy

发明人： James B. Delehanty ,  Michael H. Stewart ,  Okhil Nag ,  Jeffrey R. Deschamps ,  Kimihiro Susumu ,  Eunkeu Oh ,  Lauren D. Field ,  Alexander L. Efros ,  Alan L. Huston ,  Igor L. Medintz ,  Philip E. Dawson

IPC分类号： G01N33/58

摘要： A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be C60 fullerene. Emission correlates with cellular membrane potential.

公开(公告)号：US10780185B2

公开(公告)日：2020-09-22

申请号：US16031094

申请日：2018-07-10

申请人： The Government of the United States of America, as represented by the Secretary of the Navy

发明人： James B. Delehanty ,  Michael H. Stewart ,  Okhil Nag ,  Jeffrey R. Deschamps ,  Kimihiro Susumu ,  Eunkeu Oh ,  Lauren D. Field ,  Alexander L. Efros ,  Alan L. Huston ,  Igor L. Medintz ,  Philip E. Dawson ,  Nashaat Rasheed ,  Parag V. Chitnis ,  John R. Cressman

IPC分类号： A61K49/22 ,  G01N33/58 ,  G01N33/542

摘要： A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be C60 fullerene. Photoacoustic emission from the construct correlates with cellular membrane potential.

公开(公告)号：US20180217153A1

公开(公告)日：2018-08-02

申请号：US15882259

申请日：2018-01-29

申请人： The Government of the United States of America, as represented by the Secretary of the Navy

发明人： James B. Delehanty ,  Michael H. Stewart ,  Okhil Nag ,  Jeffrey R. Deschamps ,  Kimihiro Susumu ,  Eunkeu Oh ,  Lauren D. Field ,  Alexander L. Efros ,  Alan L. Huston ,  Igor L. Medintz ,  Philip E. Dawson

IPC分类号： G01N33/58

摘要： A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be C60 fullerene. Emission correlates with cellular membrane potential.