公开(公告)号：US20230100744A1

公开(公告)日：2023-03-30

申请号：US17802352

申请日：2021-02-26

申请人： WINDMIL THERAPEUTICS, INC. ,  THE JOHNS HOPKINS UNIVERSITY

发明人： Ivan R. Borrello ,  Kimberly A. Noonan

IPC分类号： A61K35/17 ,  C07K14/575 ,  C07K14/705 ,  C12N5/0783 ,  C07K14/54

摘要： A method for enriching or isolating tumor specific MILs is described. This method includes the steps of preparing MILs from the bone marrow of a cancer patient; evaluating the MILs for gene expression, metabolic profile, or phenotype; and selecting and isolating the MILs that exhibit the gene expression, metabolic profile, or phenotype. Compositions containing the MILs and methods of treating cancer with the enriched MILs are also described.

公开(公告)号：US20180200367A1

公开(公告)日：2018-07-19

申请号：US15742684

申请日：2016-07-08

申请人： The Johns Hopkins University

发明人： Ivan M. Borrello ,  Susan Lee ,  Kimberly A. Noonan ,  Drew M. Pardoll

IPC分类号： A61K39/395 ,  C07K14/47 ,  C07K14/725 ,  C07K14/705

CPC分类号： A61K39/39558 ,  A61K35/17 ,  C07K14/4748 ,  C07K14/7051 ,  C07K14/70521 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/03 ,  C12N5/0635 ,  C12N5/0636 ,  C12N2510/00

摘要： In some embodiments, marrow-infiltrating lymphocytes (“MILs”) comprising a chimeric antigen receptor (“CAR”) are provided. In some aspects, the embodiments relate to a method for making a recombinant MIL, comprising obtaining bone marrow comprising MILs; and transfecting, transforming, or transducing the MILs with a nucleic acid encoding a chimeric antigen receptor. In some aspects, the embodiments relate to a method for treating a condition in a subject, comprising administering to the subject a MIL comprising a CAR.

公开(公告)号：US20170224691A1

公开(公告)日：2017-08-10

申请号：US15269305

申请日：2016-09-19

申请人： The Johns Hopkins University

发明人： Ivan M. Borrello ,  Paolo Serafini ,  Kimberly A. Noonan ,  Vincenzo Bronte

IPC分类号： A61K31/53 ,  G01N33/50 ,  A61K31/519 ,  A61K31/4985 ,  A61K45/06 ,  A61K39/00

CPC分类号： A61K31/53 ,  A61K31/4985 ,  A61K31/519 ,  A61K39/0011 ,  A61K45/06 ,  G01N33/5091 ,  G01N2800/52 ,  A61K2300/00

摘要： The invention features methods and compositions featuring a PDE5 inhibitor for treating or preventing immunological-mediated disease in a subject.

公开(公告)号：US20230016031A1

公开(公告)日：2023-01-19

申请号：US17945729

申请日：2022-09-15

申请人： The Johns Hopkins University

发明人： Ivan M. Borrello ,  Kimberly A. Noonan ,  Drew M. Pardoll ,  Valentina Hoyos Velez

IPC分类号： A61K39/395 ,  C07K14/725 ,  C07K14/705 ,  C07K14/47 ,  A61K35/17 ,  C12N5/0781 ,  C12N5/0783

摘要： In some embodiments, marrow-infiltrating lymphocytes (“MILs”) comprising a chimeric antigen receptor (“CAR”) are provided. In some aspects, the embodiments relate to a method for making a recombinant MIL, comprising obtaining bone marrow comprising MILs; and transfecting, transforming, or transducing the MILs with a nucleic acid encoding a chimeric antigen receptor. In some aspects, the embodiments relate to a method for treating a condition in a subject, comprising administering to the subject a MIL comprising a CAR.

公开(公告)号：US20190298768A1

公开(公告)日：2019-10-03

申请号：US16207864

申请日：2018-12-03

申请人： The Johns Hopkins University

发明人： Ivan M. Borrello ,  Kimberly A. Noonan

IPC分类号： A61K35/17 ,  C12N5/077 ,  C12N5/0783 ,  A61P35/00

摘要： In some aspects, the invention relates to compositions comprising marrow infiltrating lymphocytes (“MILs”). The MILs may be activated MILs. In some aspects, the invention relates to methods for activating MILs, comprising incubating MILs in an environment comprising less than 21% oxygen. In some aspects, the invention relates to methods for treating cancer in a subject, comprising administering to the subject a composition comprising activated MILs.

公开(公告)号：US10406178B2

公开(公告)日：2019-09-10

申请号：US15606766

申请日：2017-05-26

申请人： The Johns Hopkins University

发明人： Ivan M. Borrello ,  Kimberly A. Noonan

IPC分类号： A61K35/17 ,  C12N5/0783 ,  C12N5/077 ,  A61P35/00 ,  A61K35/12

摘要： In some aspects, the invention relates to compositions comprising marrow infiltrating lymphocytes (“MILs”). The MILs may be activated MILs. In some aspects, the invention relates to methods for activating MILs, comprising incubating MILs in an environment comprising less than 21% oxygen. In some aspects, the invention relates to methods for treating cancer in a subject, comprising administering to the subject a composition comprising activated MILs.

公开(公告)号：US20150320798A1

公开(公告)日：2015-11-12

申请号：US14647358

申请日：2013-11-26

申请人： The Johns Hopkins University

发明人： Ivan M. Borrello ,  Kimberly A. Noonan ,  Leonido Luznik

IPC分类号： A61K35/17 ,  A61K31/675

CPC分类号： A61K35/17 ,  A61K31/675 ,  A61K35/28 ,  A61K35/51 ,  A61K39/0011 ,  A61K2039/5158

摘要： The present invention relates to the field of cancer therapy. More specifically, the present invention provides methods and compositions useful for augmenting anti-tumor immunity. In one embodiment, a method for treating or preventing post-allogeneic transplant relapse in a subject who has received post-transplant cyclophosphamide treatment comprises the steps of (a) obtaining a bone marrow sample from the subject; (b) expanding the marrow infiltrating lymphocytes (MILs) present in the sample; and (c) administering the MILs to the subject. In a specific embodiment, the method significantly reduces the likelihood of developing GVHD.

摘要翻译： 本发明涉及癌症治疗领域。 更具体地，本发明提供了可用于增强抗肿瘤免疫性的方法和组合物。 在一个实施方案中，用于治疗或预防接受移植后环磷酰胺治疗的受试者的同种异体移植物复发的方法包括以下步骤：（a）从受试者获得骨髓样品; （b）扩大样品中存在的骨髓浸润淋巴细胞（MIL）; 和（c）向受试者施用MIL。 在具体实施方案中，该方法显着降低了发生GVHD的可能性。

公开(公告)号：US20230210990A1

公开(公告)日：2023-07-06

申请号：US18072164

申请日：2022-11-30

申请人： The Johns Hopkins University

发明人： Ivan M. Borrello ,  Kimberly A. Noonan ,  Drew M. Pardoll ,  Valentina Hoyos Velez

IPC分类号： A61K39/395 ,  C07K14/725 ,  C07K14/705 ,  C07K14/47 ,  A61K35/17 ,  C12N5/0781 ,  C12N5/0783

CPC分类号： A61K39/39558 ,  C07K14/7051 ,  C07K14/70521 ,  C07K14/4748 ,  A61K35/17 ,  C12N5/0635 ,  C12N5/0636 ,  C07K2319/02 ,  C07K2319/03 ,  C12N2510/00 ,  C07K2317/622

摘要： In some embodiments, marrow-infiltrating lymphocytes (“MILs”) comprising a chimeric antigen receptor (“CAR”) are provided. In some aspects, the embodiments relate to a MIL obtained from bone marrow comprising a chimeric antigen receptor. In some aspects, the embodiments relate to a method for treating a condition in a subject, comprising administering to the subject a MIL comprising a CAR.

公开(公告)号：US11478548B2

公开(公告)日：2022-10-25

申请号：US15742684

申请日：2016-07-08

申请人： The Johns Hopkins University

发明人： Ivan M. Borrello ,  Kimberly A. Noonan ,  Drew M. Pardoll ,  Valentina Hoyos Velez

IPC分类号： A61K39/395 ,  C07K14/725 ,  C07K14/705 ,  C07K14/47 ,  A61K35/17 ,  C12N5/0781 ,  C12N5/0783

摘要： In some embodiments, marrow-infiltrating lymphocytes (“MILs”) comprising a chimeric antigen receptor (“CAR”) are provided. In some aspects, the embodiments relate to a method for making a recombinant MIL, comprising obtaining bone marrow comprising MILs; and transfecting, transforming, or transducing the MILs with a nucleic acid encoding a chimeric antigen receptor. In some aspects, the embodiments relate to a method for treating a condition in a subject, comprising administering to the subject a MIL comprising a CAR.

公开(公告)号：US20170266232A1

公开(公告)日：2017-09-21

申请号：US15606951

申请日：2017-05-26

申请人： The Johns Hopkins University

发明人： Ivan M. Borrello ,  Kimberly A. Noonan

IPC分类号： A61K35/17

CPC分类号： A61K35/17 ,  A61K2035/124 ,  A61P35/00 ,  C12N5/0636 ,  C12N5/0669 ,  C12N2500/02 ,  C12N2501/2302 ,  C12N2501/998

摘要： In some aspects, the invention relates to compositions comprising marrow infiltrating lymphocytes (“MILs”). The MILs may be activated MILs. In some aspects, the invention relates to methods for activating MILs, comprising incubating MILs in an environment comprising less than 21% oxygen. In some aspects, the invention relates to methods for treating cancer in a subject, comprising administering to the subject a composition comprising activated MILs.