公开(公告)号：US09963751B2

公开(公告)日：2018-05-08

申请号：US15355643

申请日：2016-11-18

申请人： THE PENN STATE RESEARCH FOUNDATION

发明人： Jianming Hu

IPC分类号： A61K39/00 ,  C07K14/005 ,  C07K14/03 ,  C07K14/125 ,  C07K14/18 ,  C12Q1/70 ,  C12Q1/68

CPC分类号： C12Q1/70 ,  C12Q1/6816 ,  C12Q1/706 ,  C12Q2525/307

摘要： Provided are compositions and methods for identifying test agents as candidate for use in reducing hepatitis B virus (HBV). The methods involve a) introducing at least one test agent into mammalian cells, the cells having one or more plasmids that encode an HBV genome, wherein the genome includes a segment encoding a mutated HBV core (HBc), wherein the HBc mutation is L60A, I126A, K96A or a combination thereof. The test agent is allowed to be in contact with the mammalian cells for a period of time. Subsequently, amounts of HBV cccDNA in the mammalian cells are determined. The method can be performed in vitro or in vivo. A reduction in cccDNA relative to a control indicates the test agent in the test container is a candidate for reducing HBV in an individual. Cell cultures divided into reaction containers that each contain a distinct test agent are also included.

公开(公告)号：US20160348188A1

公开(公告)日：2016-12-01

申请号：US15155503

申请日：2016-05-16

申请人： The Penn State Research Foundation

发明人： Jianming Hu

IPC分类号： C12Q1/70 ,  C12N7/00 ,  G01N33/569 ,  C07K14/005

CPC分类号： C12Q1/706 ,  C07K14/005 ,  C12N7/00 ,  C12N2730/10122 ,  G01N33/56983 ,  G01N33/5762 ,  G01N2333/005 ,  G01N2500/20

摘要： Cell-free translation and assembly systems allow for HBV capsid assembly under cell-free conditions that also mimic the physiological salt and protein concentrations. These hepatitis virus capsid assembly systems utilize the C-terminal domain (CTD) and N-terminal domain (NTD) of the hepatitis capsid protein in capsid assembly. Uses of the system include the identification of potential new therapeutic strategies and screening of potential therapeutic agents which target these domains and modulate capsid assembly and/or disassembly and other functions of these domains in HBV replication and pathogenesis.

摘要翻译： 无细胞翻译和组装系统允许在无细胞条件下也可模拟生理盐和蛋白质浓度的HBV衣壳装配。 这些肝炎病毒衣壳装配系统利用衣壳装配中的肝炎衣壳蛋白的C-末端结构域（CTD）和N-末端结构域（NTD）。 系统的使用包括鉴定潜在的新治疗策略和筛选靶向这些结构域的潜在治疗剂，并调节这些结构域在HBV复制和发病机制中的衣壳组装和/或拆解等功能。

公开(公告)号：US20170145521A1

公开(公告)日：2017-05-25

申请号：US15355643

申请日：2016-11-18

申请人： THE PENN STATE RESEARCH FOUNDATION

发明人： Jianming Hu

IPC分类号： C12Q1/70

CPC分类号： C12Q1/70 ,  C12Q1/6816 ,  C12Q1/706 ,  C12Q2525/307

摘要： Provided are compositions and methods for identifying test agents as candidate for use in reducing hepatitis B virus (HBV). The methods involve a) introducing at least one test agent into mammalian cells, the cells having one or more plasmids that encode an HBV genome, wherein the genome includes a segment encoding a mutated HBV core (HBc), wherein the HBc mutation is L60A, I126A, K96A or a combination thereof. The test agent is allowed to be in contact with the mammalian cells for a period of time. Subsequently, amounts of HBV cccDNA in the mammalian cells are determined. The method can be performed in vitro or in vivo. A reduction in cccDNA relative to a control indicates the test agent in the test container is a candidate for reducing HBV in an individual. Cell cultures divided into reaction containers that each contain a distinct test agent are also included.

公开(公告)号：US10196701B2

公开(公告)日：2019-02-05

申请号：US15155503

申请日：2016-05-16

申请人： The Penn State Research Foundation

发明人： Jianming Hu

IPC分类号： C12Q1/70 ,  C07K14/005 ,  C12N7/00 ,  G01N33/569 ,  G01N33/576

摘要： Cell-free translation and assembly systems allow for HBV capsid assembly under cell-free conditions that also mimic the physiological salt and protein concentrations. These hepatitis virus capsid assembly systems utilize the C-terminal domain (CTD) and N-terminal domain (NTD) of the hepatitis capsid protein in capsid assembly. Uses of the system include the identification of potential new therapeutic strategies and screening of potential therapeutic agents which target these domains and modulate capsid assembly and/or disassembly and other functions of these domains in HBV replication and pathogenesis.