公开(公告)号：US20240352486A1

公开(公告)日：2024-10-24

申请号：US18754912

申请日：2024-06-26

Applicant: The Trustees of Princeton University

Inventor： Cliff BRANGWYNNE ,  Yongdae SHIN ,  Dan BRACHA

IPC: C12N15/90 ,  C07K14/47 ,  C12N9/22

CPC classification number: C12N15/90 ,  C07K14/47 ,  C12N9/22

Abstract: Disclosed is a system and method for the controlled clustering of gene regulatory proteins at the specific target genomic loci. The technology enables formation of liquid-like droplets closely interfaced with genome organization. This technology can be utilized for perturbing endogenous nuclear structures as well as nucleating synthetic membrane-less assemblies. The technology can also be utilized for inducing or detecting mechanical stresses within the genome.

公开(公告)号：US20240302350A1

公开(公告)日：2024-09-12

申请号：US18273816

申请日：2022-01-21

Applicant: The Trustees of Princeton University

Inventor： Clifford P. BRANGWYNNE ,  Chanelle JUMPER ,  Paul ACKERMAN ,  Dan BRACHA

IPC: G01N33/50 ,  G01N33/68

CPC classification number: G01N33/5038 ,  G01N33/6845

Abstract: Provided herein are methods of identifying an inhibitory activity of an exogeneous agent that disrupts binding of a target peptide to a biomolecule using phase separation as in cellulo read-out. Provided herein also are kits for the methods, pharmaceutical compositions comprising the agents screened by the methods, methods of preparing the pharmaceutical composition, and methods of treating a disease or condition in a subject in need thereof by administering the pharmaceutical composition.

公开(公告)号：US20210047659A1

公开(公告)日：2021-02-18

申请号：US16964052

申请日：2019-01-23

Applicant: The Trustees of Princeton University

Inventor： Cliff BRANGWYNNE ,  Yongdae SHIN ,  Dan BRACHA

IPC: C12N15/90 ,  C12N9/22 ,  C07K14/47

Abstract: Disclosed is a system and method for the controlled clustering of gene regulatory proteins at the specific target genomic loci. The technology enables formation of liquid-like droplets closely interfaced with genome organization. This technology can be utilized for perturbing endogenous nuclear structures as well as nucleating synthetic membrane-less assemblies. The technology can also be utilized for inducing or detecting mechanical stresses within the genome.