公开(公告)号：US20190385832A1

公开(公告)日：2019-12-19

申请号：US16169900

申请日：2018-10-24

Applicant: Thermo Finnigan LLC

Inventor： Harald OSER ,  Qingyu SONG ,  Mikhail V. UGAROV

IPC: H01J49/42

Abstract: A method of operating a quadrupole mass filter is disclosed. A first set of RF and resolving DC voltages are applied to electrodes of a quadrupole mass filter to selectively transmit first ions having a first mass-to-charge ratio (m/z). A second set of RF and resolving DC voltages are applied to electrodes of the quadrupole mass filter to selectively transmit second ions having a second m/z. Detection of the second ions is initiated after completion of a settling time. The settling time is determined in accordance with the relationship: Eq. 1, where ts is the settling time, (m/z)1 is the first mass-to-charge ratio, (m/z)2 is the second mass-to-charge ratio and A, B and C are empirically derived coefficients.

公开(公告)号：US20170162371A1

公开(公告)日：2017-06-08

申请号：US14963123

申请日：2015-12-08

Applicant: Thermo Finnigan LLC

Inventor： Alan E. SCHOEN ,  Harald OSER

IPC: H01J49/00 ,  H01J49/42

CPC classification number: H01J49/005 ,  H01J49/0031 ,  H01J49/421

Abstract: A method for operating a mass spectrometer so as to detect or quantify analytes, comprises: (a) identifying a selected-reaction-monitoring (SRM) transition to be used for each respective analyte; (b) determining a time duration required for a fragmentation reaction corresponding to each identified transition to proceed to a threshold percentage of completion; and (c) for each analyte, performing the steps of (i) isolating ions corresponding to a precursor-ion mass-to-charge (m/z) ratio of the respective transition; (ii) fragmenting the respective isolated ions in one of two fragmentation cells or fragmentation cell portions; and (ii) mass analyzing for fragment ions corresponding to a product-ion m/z ratio of the respective transition, wherein, for each analyte, the fragmentation cell or fragmentation cell portion that is used for fragmenting the isolated ions is determined from the time duration determined for the respective analyte.

公开(公告)号：US20150364302A1

公开(公告)日：2015-12-17

申请号：US14742115

申请日：2015-06-17

Applicant: Thermo Finnigan LLC

Inventor： Oleg SILIVRA ,  Harald OSER ,  Joshua T. MAZE ,  Terry OLNEY ,  Alan E. SCHOEN

IPC: H01J49/00 ,  H01J49/42

CPC classification number: H01J49/005 ,  H01J49/4215 ,  H01J49/4225 ,  H01J49/429

Abstract: A collision cell has a plurality of rod electrodes arranged in opposed pairs around an axial centerline and a plurality of drag vanes arranged in the interstitial spaces between the rod electrodes. Operating the collision cell includes, applying a rod offset voltage to the rod electrodes, and varying an offset voltage applied to the drag vanes to identify a vane offset voltage with a maximum intensity for the transition. The method further includes varying a drag field by adjusting the voltages applied to drag vane terminals in opposite directions to identify a drag field value with a cross talk below a cross talk threshold, varying the vane offset voltage by adjusting the voltages applied to the drag vane terminals to maximize the intensity of the transition while preserving the drag field, and operating the collision cell at the vane offset voltage and drag field to monitor the transition.

Abstract translation: 碰撞单元具有围绕轴向中心线相对排列的多个杆电极以及布置在杆电极之间的间隙空间中的多个牵引叶片。 操作碰撞单元包括：向杆电极施加杆偏移电压，以及改变施加到牵引叶片的偏移电压，以识别具有用于转变的最大强度的叶片偏移电压。 该方法还包括通过调整施加到相反方向上的牵引叶片端子的电压来改变阻力场，以通过串扰低于串扰阈值来识别阻力场值，通过调节施加到牵引叶片的电压来改变叶片偏移电压 终端，以最大化过渡的强度，同时保持拖曳场，并且在叶片偏移电压和阻力场处操作碰撞单元以监视转换。

公开(公告)号：US20200227244A1

公开(公告)日：2020-07-16

申请号：US16831685

申请日：2020-03-26

Applicant: Thermo Finnigan LLC

Inventor： Bennett S. KALAFUT ,  Harald OSER

IPC: H01J49/00 ,  H01J49/42

Abstract: A mass spectrometer includes a collision cell and a system controller. The collision cell includes a plurality of rod pairs configured to generate pseudopotential well through the application of radio frequency potentials to the rod pairs. The collision cell configured to generate a target fragment from a parent ion by colliding the parent ion with one or more gas molecules. The system controller is configured to set a radio frequency amplitude of the radio frequency potentials to a default amplitude; monitor the production of a target fragment ion while adjusting the collision energy; set the collision energy to optimize the production of the target fragment ion; apply a linear full range ramp to the radio frequency amplitude to determine an optimal radio frequency amplitude; and set the radio frequency amplitude to the optimal radio frequency amplitude for the parent ion, target fragment ion pair.

公开(公告)号：US20190074168A1

公开(公告)日：2019-03-07

申请号：US16181280

申请日：2018-11-05

Applicant: Thermo Finnigan LLC

Inventor： Bennett S. KALAFUT ,  Harald OSER

IPC: H01J49/00 ,  H01J49/42

Abstract: A mass spectrometer includes a collision cell and a system controller. The collision cell includes a plurality of rod pairs configured to generate pseudopotential well through the application of radio frequency potentials to the rod pairs. The collision cell configured to generate a target fragment from a parent ion by colliding the parent ion with one or more gas molecules. The system controller is configured to set a radio frequency amplitude of the radio frequency potentials to a default amplitude; monitor the production of a target fragment ion while adjusting the collision energy; set the collision energy to optimize the production of the target fragment ion; apply a linear full range ramp to the radio frequency amplitude to determine an optimal radio frequency amplitude; and set the radio frequency amplitude to the optimal radio frequency amplitude for the parent ion, target fragment ion pair.

公开(公告)号：US20150179419A1

公开(公告)日：2015-06-25

申请号：US14138589

申请日：2013-12-23

Applicant: Thermo Finnigan LLC

Inventor： Oleg SILIVRA ,  Harald OSER ,  Terry N. OLNEY

IPC: H01J49/00 ,  H01J49/42

CPC classification number: H01J49/0031 ,  H01J49/0036 ,  H01J49/004

Abstract: A mass spectrometry method comprises: introducing a first portion of a sample of ions including precursor ions comprising a first precursor-ion mass-to-charge (m/z) ratio into a first mass analyzer; transmitting the precursor ions from the first mass analyzer to a reaction or fragmentation cell such that a first population of product ions are continuously accumulated therein over a first accumulation time duration; initiating release of the accumulated first population of product ions from the reaction or fragmentation cell; continuously transmitting the released first population of product ions from the reaction cell to a second mass analyzer; transmitting a portion of the released first population of product ions comprising a first product-ion m/z ratio from the second mass analyzer to a detector; and detecting a varying quantity of the product ions having the first product-ion m/z ratio for a predetermined data-acquisition time period after the initiation of the release.

Abstract translation: 一种质谱法包括：将包含第一前体 - 离子质量 - 电荷（m / z）比率的前体离子的离子样品的第一部分引入第一质量分析器; 将所述前体离子从所述第一质量分析器传输到反应或破碎单元，使得第一种产物离子在第一累积持续时间内连续地累积在其中; 开始从反应或碎裂细胞释放累积的第一批产物离子; 将释放的第一批产物离子从反应池连续传输到第二质量分析器; 将包含第一产物离子m / z比率的所述释放的第一产物离子群的一部分从所述第二质量分析器传输到检测器; 以及在释放开始之后，在预定的数据采集时间段内检测具有第一乘积离子m / z比的产物离子的变化量。

公开(公告)号：US20230360899A1

公开(公告)日：2023-11-09

申请号：US18351270

申请日：2023-07-12

Applicant: Thermo Finnigan LLC

Inventor： Paul I. HENDRICKS ,  Harald OSER

IPC: H01J49/00 ,  H01J49/42 ,  H01J49/06

CPC classification number: H01J49/005 ,  H01J49/063 ,  H01J49/4225 ,  H01J49/426

Abstract: A multipole ion guide includes a plurality of electrodes disposed about a longitudinal axis of the device so as to define an ion transmission volume for transmitting ions along a length of the device between opposite inlet and outlet ends. An electronic controller is operably connected to an RF power source and to at least some of the electrodes and is configured to apply at least an RF potential to the electrodes. During use the electrodes generate an RF-only field along a first portion of the device and an axial DC field along a second portion of the device. Ions are focused radially inward toward the longitudinal axis of the device by the RF-only field within the first portion of the device prior to and/or subsequent to experiencing the axial DC field within the second portion of the device.

公开(公告)号：US20180254174A1

公开(公告)日：2018-09-06

申请号：US15446756

申请日：2017-03-01

Applicant: Thermo Finnigan LLC

Inventor： Bennett S. KALAFUT ,  Harald OSER

IPC: H01J49/00 ,  H01J49/42

CPC classification number: H01J49/005 ,  H01J49/0009 ,  H01J49/0031 ,  H01J49/421

Abstract: A mass spectrometer includes a collision cell and a system controller. The collision cell includes a plurality of rod pairs configured to generate pseudopotential well through the application of radio frequency potentials to the rod pairs. The collision cell configured to generate a target fragment from a parent ion by colliding the parent ion with one or more gas molecules. The system controller is configured to set a radio frequency amplitude of the radio frequency potentials to a default amplitude; monitor the production of a target fragment ion while adjusting the collision energy; set the collision energy to optimize the production of the target fragment ion; apply a linear full range ramp to the radio frequency amplitude to determine an optimal radio frequency amplitude; and set the radio frequency amplitude to the optimal radio frequency amplitude for the parent ion, target fragment ion pair.

公开(公告)号：US20180102239A1

公开(公告)日：2018-04-12

申请号：US15836101

申请日：2017-12-08

Applicant: Thermo Finnigan LLC

Inventor： Alan E. SCHOEN ,  Harald OSER

IPC: H01J49/00 ,  H01J49/42

CPC classification number: H01J49/005 ,  H01J49/0031 ,  H01J49/421

Abstract: A mass spectrometer system comprises: (a) an ion source; (b) a mass filter; (c) a mass analyzer; (d) a partitioned ion fragmentation cell configured to receive ions from the mass filter and to outlet fragment ions to the mass analyzer comprising: (d1) a set of multipole rod electrodes; a housing enclosing the set of multipole rod electrodes and comprising an ion inlet and an ion outlet; (d2) a set of partitions within the housing separating the housing interior into a plurality of compartments; and (d3) a plurality of gas inlets, each gas inlet fluidically coupled to a source of a collision gas and to a respective compartment and having a respective inlet shutoff valve; and (e) a controller electrically coupled to each inlet shutoff valve and configured to independently control the pressure of collision gas within each compartment.

公开(公告)号：US20160133448A1

公开(公告)日：2016-05-12

申请号：US14535937

申请日：2014-11-07

Applicant: Thermo Finnigan LLC

Inventor： Oleg SILIVRA ,  Harald OSER ,  Joshua T. MAZE

IPC: H01J49/00 ,  H01J49/02

CPC classification number: H01J49/0009 ,  H01J43/04 ,  H01J49/025

Abstract: Method for operating a mass spectrometer includes supplying a quantity of ions to an ion detector. The ion detector can include a conversion dynode operating in a first polarity and an electron multiplier. The method further includes adjusting the gain of the electron multiplier to determine a first set of calibration parameters, and calculating a second set of calibration parameters for the electron multiplier from the first set of calibration parameters. The second set of calibration parameters are for a second polarity of the conversion dynode. The method can further include configuring the ion detector to operate at the second polarity based on the second set of calibration parameters, and supplying ions of the second polarity to the mass spectrometer, and detecting an ion at a particular mass to charge ratio using the ion detector.

Abstract translation: 用于操作质谱仪的方法包括向离子检测器提供一定量的离子。 离子检测器可以包括以第一极性操作的转换倍增电极和电子倍增器。 该方法还包括调整电子倍增器的增益以确定第一组校准参数，以及从第一组校准参数计算电子倍增器的第二组校准参数。 第二组校准参数用于转换倍增电极的第二极性。 该方法还可以包括基于第二组校准参数将离子检测器配置为以第二极性操作，并将第二极性的离子提供给质谱仪，并使用离子检测特定质荷比的离子 探测器。