公开(公告)号：US07378057B2

公开(公告)日：2008-05-27

申请号：US10394825

申请日：2003-03-21

申请人： Thomas Arter ,  Scott Schwallie ,  James Hassett ,  James Love ,  James D. Shaw ,  Donald J. Moran, Jr. ,  Robert T. Witkowski ,  Malinda L. McFeaters

发明人： Thomas Arter ,  Scott Schwallie ,  James Hassett ,  James Love ,  James D. Shaw ,  Donald J. Moran, Jr. ,  Robert T. Witkowski ,  Malinda L. McFeaters

IPC分类号： B01L3/02

CPC分类号： B01L3/08 ,  B01L2200/026 ,  B01L2200/142 ,  B01L2200/16 ,  G01N2035/00287 ,  Y10T436/2575

摘要： Evaporation control for a fluid supply, such as used in a clinical analyzer, can be provided by utilizing a straw-like insert which is disposed into a fluid-containing bottle. The straw provides an increased diffusion length or “stagnant” zone. The bottle can also be configured to effectively reduce evaporation in a similar manner by providing a narrowed diameter prior to the fluid retaining portion of the bottle and by increasing the distance between the top of the bottle and the top of the fluid surface.

摘要翻译： 用于流体供应的蒸发控制，例如在临床分析仪中使用的，可以通过利用设置在流体容纳瓶中的吸管状插入物来提供。 秸秆增加扩散长度或“停滞”区域。 瓶子还可以被配置为通过在瓶子的流体保持部分之前提供变窄的直径并且通过增加瓶子的顶部和流体表面的顶部之间的距离，以类似的方式有效地减少蒸发。

公开(公告)号：US20060154372A1

公开(公告)日：2006-07-13

申请号：US11302660

申请日：2005-12-14

申请人： Thomas Arter ,  Merrit Jacobs

发明人： Thomas Arter ,  Merrit Jacobs

IPC分类号： G01N35/00

CPC分类号： G01N35/00 ,  G01N2035/00633 ,  Y10T436/11

摘要： A method of providing motion to a sample during a reaction phase in an incubator of a clinical analyzer includes: providing an analyzer containing an incubator, wherein the incubator has one or more cells for containing sample and optionally one or more reagents; moving the incubator to position one or more cells to perform an operation, the operation includes dispensing a sample and optionally one or more reagents into each of the one or more cells; and additionally moving the incubator in such a manner that the number of motions of the one or more cells during the reaction phase for an assay does not substantially change as a function of the number of samples being analyzed in the incubator or the order of the sample in the incubator for the same assay. Also disclosed is a method for increasing precision for multiple assays in a clinical analyzer, which includes: providing an analyzer containing an incubator, wherein the incubator has two or more cells for containing sample to be assayed and optionally one or more reagents; moving the incubator to position the two or more cells to perform an operation, the operation includes dispensing a sample and optionally one or more reagents into each of the two or more cells; and additionally moving the sample prior to performing a measurement of the sample, such that samples receiving the step of additionally moving have greater precision than samples which do not receive the step of additionally moving.

摘要翻译： 在临床分析仪的培养箱中的反应阶段期间向样品提供运动的方法包括：提供含有培养箱的分析器，其中培养箱具有一个或多个用于容纳样品和任选的一种或多种试剂的细胞; 移动培养器以定位一个或多个细胞以进行操作，所述操作包括将样品和任选的一种或多种试剂分配到所述一个或多个细胞的每一个中; 并且另外移动培养箱，使得在用于测定的反应阶段期间的一个或多个细胞的运动次数基本上不随培养箱中分析的样品数量或样品的顺序而变化 在孵化器中进行相同的检测。 还公开了一种用于在临床分析仪中提高多个测定的精度的方法，其包括：提供包含培养箱的分析仪，其中培养箱具有两个或更多个用于包含要测定的样品的细胞和任选的一种或多种试剂; 移动培养器以定位两个或更多个细胞以进行操作，操作包括将样品和任选的一种或多种试剂分配到两个或更多个细胞中的每一个中; 并且在进行样本的测量之前另外移动样本，使得接收附加移动步骤的样本具有比不接收附加移动步骤的样本更高的精度。

公开(公告)号：US20050037504A1

公开(公告)日：2005-02-17

申请号：US10890588

申请日：2004-07-14

申请人： Theodore DiMagno ,  Thomas Arter

发明人： Theodore DiMagno ,  Thomas Arter

IPC分类号： G01N33/92 ,  B01D39/00 ,  C12Q1/60 ,  G01N33/52 ,  G01N33/543

CPC分类号： G01N33/526 ,  C12Q1/60

摘要： Disclosed is a method for quantifying cholesterol in high-density lipoproteins in a single step assay using a dry slide. The method for quantifying cholesterol in high-density lipoprotein comprises a first step of adding a sample onto a multi-layered dry slide wherein at least one of the layers contains phosphotungstic acid and another contains a high-density lipoprotein selective surfactant. The phosphotungstic acid precipitates non-high-density lipoproteins while the high-density lipoprotein selective surfactant only solubilizes high-density lipoproteins and does not solubilize non-HDL precipitated complexes. The cholesterol esterase then reacts with the solubilized HDL cholesterol esters to form cholesterol. Finally the cholesterol in the high-density lipoprotein is detected and quantified.

摘要翻译： 公开了一种使用干燥载玻片在单步测定中定量高密度脂蛋白中的胆固醇的方法。 用于定量高密度脂蛋白胆固醇的方法包括将样品添加到多层干燥载玻片上的第一步骤，其中至少一层包含磷钨酸，另一层含有高密度脂蛋白选择性表面活性剂。 磷钨酸沉淀非高密度脂蛋白，而高密度脂蛋白选择性表面活性剂仅溶解高密度脂蛋白，并且不溶解非HDL沉淀复合物。 胆固醇酯酶然后与溶解的HDL胆固醇酯反应形成胆固醇。 最后检测和量化高密度脂蛋白中的胆固醇。

公开(公告)号：US6046052A

公开(公告)日：2000-04-04

申请号：US65930

申请日：1998-04-24

申请人： Thomas Arter ,  David B. LaTart ,  John C. Mauck ,  Richard C. Sutton ,  Wayne Weber ,  Robert Winterkorn ,  James Schaeffer

发明人： Thomas Arter ,  David B. LaTart ,  John C. Mauck ,  Richard C. Sutton ,  Wayne Weber ,  Robert Winterkorn ,  James Schaeffer

IPC分类号： G01N21/78 ,  G01N33/52 ,  G01N33/68 ,  G01N33/48

CPC分类号： G01N33/6827 ,  G01N33/525 ,  G01N33/683 ,  G01N33/6839

摘要： A dry analytical element is disclosed which can be used to sensitively and rapidly detect and quantitate protein. The assays are carried out using a dye that reacts with protein and molybdate ion to produce a measurable change in the spectral absorption of the dye. Also disclosed are polymers which stabilize and enhance the accuracy of the assay, and compounds which reduce interference by bicarbonate.

摘要翻译： 公开了可用于敏感和快速检测和定量蛋白质的干燥分析元件。 使用与蛋白质和钼酸根离子反应的染料进行测定以产生染料的光谱吸收的可测量的变化。 还公开了稳定和提高测定精度的聚合物，以及减少碳酸氢盐干扰的化合物。

公开(公告)号：US20050032141A1

公开(公告)日：2005-02-10

申请号：US10890610

申请日：2004-07-14

申请人： Theodore DiMagno ,  Thomas Arter ,  Deborah Chambers ,  David Silva ,  Karen Vavra

发明人： Theodore DiMagno ,  Thomas Arter ,  Deborah Chambers ,  David Silva ,  Karen Vavra

IPC分类号： G01N33/52 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/44 ,  C12Q1/60 ,  G01N33/92

CPC分类号： G01N33/92

摘要： A dry analytical element is disclosed which can be used for the quantification of high-density lipoprotein cholesterol (HDLC). The element comprises a support having one or more reagent layers containing a first enzyme, a cholesterol ester hydrolase, to hydrolyze cholesterol esters, a second enzyme, cholesterol oxidase, to release hydrogen peroxide from cholesterol, and a third enzyme, horseradish peroxidase, to oxidize a leuco dye that is read at 670 nm. The element also contains phosphotungstic acid, a non-high-density lipoprotein precipitant, and a high-density lipoprotein selective surfactant, which together confer HDLC selectivity on the assay. Also disclosed are polymers that improve assay precision and eliminate interference from hemolyzed patient samples.

摘要翻译： 公开了可用于定量高密度脂蛋白胆固醇（HDLC）的干燥分析元件。 该元件包括具有一个或多个试剂层的载体，其含有第一种酶，胆固醇酯水解酶，水解胆固醇酯，第二种酶，胆固醇氧化酶，以从胆固醇中释放过氧化氢，以及第三种酶，辣根过氧化物酶氧化 在670nm读取的无色染料。 该元件还含有磷钨酸，非高密度脂蛋白沉淀剂和高密度脂蛋白选择性表面活性剂，它们共同为测定提供HDLC选择性。 还公开了提高测定精度并消除来自溶血患者样品的干扰的聚合物。

公开(公告)号：US20050074828A1

公开(公告)日：2005-04-07

申请号：US10890613

申请日：2004-07-14

申请人： Theodore DiMagno ,  Thomas Arter

发明人： Theodore DiMagno ,  Thomas Arter

IPC分类号： C12Q1/60 ,  G01N33/92

CPC分类号： G01N33/92

摘要： The present invention relates to a dry analytical element useful for the determination and quantification of high-density lipoprotein cholesterol (HDLC) that uses yeast lipase from Candida rugosa, a lipase enzyme source that has cholesterol esterase activity, which preferentially reacts with the cholesterol esters of HDL over cholesterol esters of other lipoproteins.

摘要翻译： 本发明涉及用于测定和定量使用具有胆固醇酯酶活性的脂肪酶源的假丝酵母（Candida rugosa）的酵母脂肪酶的高密度脂蛋白胆固醇（HDLC）的干燥分析元件，其优先与胆固醇酯类 HDL超过其他脂蛋白的胆固醇酯。