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公开(公告)号:US20130040302A1
公开(公告)日:2013-02-14
申请号:US13546365
申请日:2012-07-11
CPC分类号: C12N5/0696 , C12N15/907 , C12N2501/15 , C12N2501/235 , C12N2501/415 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2501/608 , C12N2501/727 , C12N2506/1307
摘要: Methods for producing engineered induced pluripotent stem (iPS) cells are provided comprising introducing a first nucleic acid into somatic cells for integration into their genome and reprogramming the cells to produce engineered iPS cells having the nucleic acid integrated into their genome. For example, in certain aspects the cells are reprogrammed by introduction of a genetic element that expresses one or more reprogramming factor and culturing of the cells under conditions sufficient to produce reprogrammed cells.
摘要翻译: 提供了用于产生工程化诱导多能干细胞(iPS)细胞的方法,包括将第一核酸引入体细胞用于整合到其基因组中并重新编程细胞以产生具有整合入其基因组中的核酸的工程化iPS细胞。 例如,在某些方面,通过引入表达一种或多种重编程因子的遗传元件并在足以产生重编程细胞的条件下培养细胞来重新编程细胞。