公开(公告)号：US07075640B2

公开(公告)日：2006-07-11

申请号：US08984979

申请日：1997-12-04

申请人： Thomas M. Baer ,  David F. Head ,  John Toeppen

发明人： Thomas M. Baer ,  David F. Head ,  John Toeppen

IPC分类号： G01N21/01

CPC分类号： G01N1/2813 ,  G01N2001/028 ,  G01N2001/2833 ,  G01N2001/284

摘要： Systems and methods for acquiring laser capture microdissection samples are disclosed. An integral portion of a biological reaction vessel includes a transfer film carrier having a substrate surface; and a laser capture microdissection transfer film coupled to the substrate surface of the transfer film carrier. The systems and methods facilitate quick and accurate laser capture microdissection while simultaneously minimizing contamination.

摘要翻译： 公开了用于获取激光捕获显微解剖样品的系统和方法。 生物反应容器的整体部分包括具有基底表面的转移膜载体; 以及耦合到转印膜载体的基板表面的激光捕获显微切割转印膜。 系统和方法有助于快速准确的激光捕获显微切割，同时最大限度地减少污染。

公开(公告)号：US07221447B2

公开(公告)日：2007-05-22

申请号：US11276887

申请日：2006-03-17

申请人： Thomas M. Baer ,  David F. Head ,  John Toeppen

发明人： Thomas M. Baer ,  David F. Head ,  John Toeppen

IPC分类号： G01N21/01

CPC分类号： G01N1/2813 ,  G01N2001/028 ,  G01N2001/2833 ,  G01N2001/284

摘要： Systems and methods for acquiring laser capture microdissection samples are disclosed. A scattering media is located within a beam path defined by the laser capture microdissection microscope and the sample is imaged through the scattering media. A transfer film carrier includes a substrate surface and a laser capture microdissection transfer film coupled to the substrate surface of the transfer film carrier. The scattering media is optically coupled to a laser capture microdissection transfer film being located in or attached to the laser capture microdissection transfer film or transfer film carrier. The systems and methods provide improved sample imaging and facilitate quick and accurate laser capture microdissection while simultaneously minimizing contamination.

摘要翻译： 公开了用于获取激光捕获显微解剖样品的系统和方法。 散射介质位于由激光捕获显微切割显微镜限定的光束路径内，并且样品通过散射介质成像。 转印膜载体包括与转印膜载体的基板表面耦合的基板表面和激光捕获显微解剖转印膜。 散射介质光学耦合到位于激光捕获显微解剖转移膜或转移膜载体中或附着于激光捕获显微解剖转移膜或转移膜载体的激光捕获显微解剖转移膜。 这些系统和方法提供了改进的样品成像，并促进了快速准确的激光捕获显微解剖，同时最大限度地减少了污染。

公开(公告)号：US5985085A

公开(公告)日：1999-11-16

申请号：US984983

申请日：1997-12-04

申请人： Thomas M. Baer ,  David F. Head ,  John Toeppen

发明人： Thomas M. Baer ,  David F. Head ,  John Toeppen

IPC分类号： G01N1/02 ,  G01N1/28 ,  B32B31/26 ,  C12Q1/00

CPC分类号： G01N1/2813 ,  G01N2001/028 ,  G01N2001/2833 ,  G01N2001/284 ,  Y10T436/25375

摘要： Systems and methods for acquiring laser capture microdissection samples are disclosed. A method of making a laser capture microdissection consumable includes providing a transfer film carrier having a substrate surface; and fabricating a laser capture microdissection transfer film on said substrate surface, wherein forming includes hot vacuum baking said laser capture microdissection transfer film. The systems and methods facilitate quick and accurate laser capture microdissection while simultaneously minimizing contamination.

摘要翻译： 公开了用于获取激光捕获显微解剖样品的系统和方法。 制造激光捕获显微解剖消耗品的方法包括提供具有基底表面的转移膜载体; 以及在所述衬底表面上制造激光捕获显微切割转移膜，其中形成包括热真空烘烤所述激光捕获显微切割转移膜。 系统和方法有助于快速准确的激光捕获显微切割，同时最大限度地减少污染。

公开(公告)号：US20060148070A1

公开(公告)日：2006-07-06

申请号：US11276887

申请日：2006-03-17

申请人： THOMAS BAER ,  David Head ,  John Toeppen

发明人： THOMAS BAER ,  David Head ,  John Toeppen

IPC分类号： C12M1/34

CPC分类号： G01N1/2813 ,  G01N2001/028 ,  G01N2001/2833 ,  G01N2001/284

摘要： Systems and methods for acquiring laser capture microdissection samples are disclosed. A scattering media is located within a beam path defined by the laser capture microdissection microscope and the sample is imaged through the scattering media. A transfer film carrier includes a substrate surface and a laser capture microdissection transfer film coupled to the substrate surface of the transfer film carrier. The scattering media is optically coupled to a laser capture microdissection transfer film being located in or attached to the laser capture microdissection transfer film or transfer film carrier. The systems and methods provide improved sample imaging and facilitate quick and accurate laser capture microdissection while simultaneously minimizing contamination.

摘要翻译： 公开了用于获取激光捕获显微解剖样品的系统和方法。 散射介质位于由激光捕获显微切割显微镜限定的光束路径内，并且样品通过散射介质成像。 转印膜载体包括与转印膜载体的基板表面耦合的基板表面和激光捕获显微解剖转印膜。 散射介质光学耦合到位于激光捕获显微解剖转移膜或转印膜载体中或连接到激光捕获显微切割转移膜或转印膜载体上的激光捕获显微解剖转移膜。 这些系统和方法提供了改进的样品成像，并促进了快速准确的激光捕获显微解剖，同时最大限度地减少了污染。

公开(公告)号：US20050046795A1

公开(公告)日：2005-03-03

申请号：US10649314

申请日：2003-08-26

申请人： John Toeppen

发明人： John Toeppen

IPC分类号： G02B27/22 ,  H04N13/00 ,  G03B21/00

CPC分类号： G02B27/225 ,  H04N13/305 ,  H04N13/363

摘要： An autostereoscopic viewer is employed to produce aberration corrected images to simulate a virtual presence by employing pairs of projector optical components coupled with an image corrector plate and a field lens. Images are designed with magnifications and optical qualities and positioned at predetermined eyezones having controlled directional properties. The viewer's eyes are positioned in these eyezones. The size of these zones is related to the aperture of the projection lenses, the Fresnels' magnification, and the optical properties and position of the image corrector plate.

摘要翻译： 使用自动立体观察器来产生像差校正图像以通过使用与图像校正板和场透镜耦合的投影仪光学组件对来模拟虚拟存在。 图像设计具有放大率和光学质量，并且位于具有受控方向特性的预定眼区。 观众的眼睛位于这些眼区。 这些区域的尺寸与投影透镜的孔径，Fresnels的放大率以及图像校正板的光学性质和位置有关。