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    NEMATODE-RESISTANT PLANTS, AND MODIFIED BACILLUS THURINGIENSIS CRY GENES AND PROTEINS
    2.
    发明申请
    NEMATODE-RESISTANT PLANTS, AND MODIFIED BACILLUS THURINGIENSIS CRY GENES AND PROTEINS 审中-公开
    抗虫植物和改良的BACILUS THURINGIENSIS CRY基因和蛋白质

    公开(公告)号：US20110239334A1

    公开(公告)日：2011-09-29

    申请号：US13060240

    申请日：2009-08-25

    申请人： Timothy D. Hey ,  Aaron T. Woosley ,  Kenneth E. Narva

    发明人： Timothy D. Hey ,  Aaron T. Woosley ,  Kenneth E. Narva

    IPC分类号： A01N37/18 ,  A01H5/00 ,  C12N15/32 ,  C07K14/325 ,  C12N5/04 ,  A01P5/00

    CPC分类号： C12N15/8285 ,  Y02A40/164

    摘要： The subject invention concerns plants protected from nematode feeding damage and improved versions of Cry proteins. Synthetic genes encoding Cry proteins are also part of the subject invention. Another embodiment of the subject invention includes plants transformed with the genes of the subject invention. In yet another embodiment the subject invention concerns Bt proteins for in-plant protection against crop damage by root knot nematode (RKN; Meloidogyne incognita) and soybean cyst nematode (SCN; Heterodera glycines).

    摘要翻译： 本发明涉及防止线虫喂养损伤和Cry蛋白质改良形式的植物。 编码Cry蛋白的合成基因也是本发明的一部分。 本发明的另一个实施方案包括用本发明的基因转化的植物。 在另一个实施方案中，本发明涉及用于通过根结线虫（RKN;不同根结线虫）和大豆胞囊线虫（SCN;异源甘氨酸）对作物损害的植物内保护的Bt蛋白质。

    COMBINATIONS INCLUDING CRY3AA AND CRY6AA PROTEINS TO PREVENT DEVELOPMENT OF RESISTANCE IN CORN ROOTWORMS (DIABROTICA SPP.)
    3.
    发明申请
    COMBINATIONS INCLUDING CRY3AA AND CRY6AA PROTEINS TO PREVENT DEVELOPMENT OF RESISTANCE IN CORN ROOTWORMS (DIABROTICA SPP.) 有权
    包括CRY3AA和CRY6AA蛋白的组合，以防止CORN ROOTWORMS（DIABROTICA SPP。）中的抗性发展

    公开(公告)号：US20130263331A1

    公开(公告)日：2013-10-03

    申请号：US13643052

    申请日：2011-04-22

    申请人： Kenneth E. Narva ,  Thomas Meade ,  Kristin J. Fencil ,  Huarong Li ,  Timothy D. Hey ,  Aaron T. Woosley ,  Monica B. Olson

    发明人： Kenneth E. Narva ,  Thomas Meade ,  Kristin J. Fencil ,  Huarong Li ,  Timothy D. Hey ,  Aaron T. Woosley ,  Monica B. Olson

    IPC分类号： C12N15/82 ,  B32B1/02

    CPC分类号： C12N15/8286 ,  A01G22/00 ,  A01H5/10 ,  A01N37/18 ,  A01N43/50 ,  B32B1/02 ,  B65D85/00 ,  C07K14/325 ,  Y02A40/162 ,  Y10T428/13

    摘要： The subject invention relates in part to Cry3Aa in combination with Cry6Aa. The subject invention relates in part to the surprising discovery that combinations of Cry3Aa and Cry6Aa are useful for preventing development of resistance (to either insecticidal protein system alone) by a corn rootworm (Diabrotica spp.) population. Included within the subject invention are plants producing these insecticidal Cry proteins, which are useful to mitigate concern that a corn rootworm population could develop that would be resistant to either of these insecticidal protein systems alone. Plants (and acreage planted with such plants) that produce these two insecticidal protein systems are included within the scope of the subject invention. The subject invention also relates in part to combinations of Cry3Aa and Cry6Aa proteins “triple-stacked” or “multi-stacked” with another insecticidal protein(s) such as a Cry6Aa protein or binary Cry34/35 proteins. Thus, such embodiments target rootworms with three modes of action. Transgenic plants, including corn, comprising a cry6Aa gene and a cry3Aa gene are included within the scope of the subject invention.

    摘要翻译： 本发明部分地涉及Cry3Aa与Cry6Aa的组合。 本发明部分地涉及令人惊奇的发现，即Cry3Aa和Cry6Aa的组合可用于防止由玉米根虫（Diabrotica spp。）种群发展抗性（仅针对任一杀虫蛋白质系统）。 本发明中包括的是产生这些杀虫Cry蛋白质的植物，其可用于减轻玉米根虫群体可能发展的担忧，这些玉米根虫群可以单独抗这些杀虫蛋白系统。 产生这两种杀虫蛋白质系统的植物（和种植有这种植物的种植面包括在本发明的范围内）。 本发明还部分涉及Cry3Aa和Cry6Aa蛋白质“三叠层”或“多堆叠”与另一种杀虫蛋白如Cry6Aa蛋白或二元Cry34 / 35蛋白的组合。 因此，这样的实施例以三种作用模式来针对根虫。 包含cry6Aa基因和cry3Aa基因的包含玉米的转基因植物包括在本发明的范围内。

    Toxins active against pests
    7.
    发明授权
    Toxins active against pests 有权
    毒性有毒物

    公开(公告)号：US06752992B2

    公开(公告)日：2004-06-22

    申请号：US10099285

    申请日：2002-03-15

    申请人： H. Ernest Schnepf ,  Carol Wicker ,  Kenneth E. Narva ,  Michele Walz ,  Brian A. Stockhoff ,  Judy Muller-Cohn

    发明人： H. Ernest Schnepf ,  Carol Wicker ,  Kenneth E. Narva ,  Michele Walz ,  Brian A. Stockhoff ,  Judy Muller-Cohn

    IPC分类号： A61K3907

    CPC分类号： C12N15/8286 ,  C07K14/325 ,  C07K2319/00 ,  C12N2799/026 ,  Y02A40/162

    摘要： The subject invention concerns materials and methods useful in the control of non-mammalian pests and, particularly, plant pests. In a specific embodiment, the subject invention provides new Bacillus thuringiensis toxins useful for the control of lepidopterans. The subject invention further provides nucleotide sequences which encode the toxins of the subject invention. The nucleotide sequences of the subject invention can be used to transform hosts, such as plants, to express the pesticidal toxins of the subject invention. The subject invention further concerns novel nucleotide primers for the identification of genes encoding toxins active against pests. The primers are useful in PCR techniques to produce gene fragments which are characteristic of genes encoding these toxins. The primers are also useful as nucleotide probes to detect the toxin-encoding genes.

    摘要翻译： 本发明涉及可用于控制非哺乳动物害虫，特别是植物害虫的材料和方法。 在具体实施方案中，本发明提供了可用于控制鳞翅目昆虫的新的苏云金芽孢杆菌毒素。 本发明进一步提供编码本发明毒素的核苷酸序列。 本发明的核苷酸序列可用于转化宿主如植物，以表达本发明的杀虫毒素。 本发明还涉及用于鉴定编码针对害虫的毒素的基因的新型核苷酸引物。 引物可用于PCR技术以产生编码这些毒素的基因特征的基因片段。 引物也可用作检测毒素编码基因的核苷酸探针。

    Bacillus thuringiensis toxins and genes for controlling coleopteran pests
    8.
    发明授权
    Bacillus thuringiensis toxins and genes for controlling coleopteran pests 失效
    苏云金芽孢杆菌毒素和防治鞘翅目害虫的基因

    公开(公告)号：US06710027B2

    公开(公告)日：2004-03-23

    申请号：US09991582

    申请日：2001-11-16

    申请人： Gregory A. Bradfisch ,  Judy Muller-Cohn ,  Kenneth E. Narva ,  Jenny M. Fu ,  Mark Thompson

    发明人： Gregory A. Bradfisch ,  Judy Muller-Cohn ,  Kenneth E. Narva ,  Jenny M. Fu ,  Mark Thompson

    IPC分类号： A01N3718

    CPC分类号： C07K14/325

    摘要： The subject invention concerns materials and methods useful in the control of pests and, particularly, plant pests. More specifically, the subject invention concerns novel genes and pesticidal toxins referred to as 86A1(b) and 52A1(b). In preferred embodiments, the subject toxins are used for controlling flea beetles of the genus Phyllotreta. Using the genes described herein, the transformation of plants can be accomplished using techniques known to those skilled in the art. In addition, the subject invention provides toxin genes optimized for expression in plants.

    摘要翻译： 本发明涉及可用于防治害虫，特别是植物害虫的材料和方法。 更具体地，本发明涉及被称为86A1（b）和52A1（b）的新基因和杀虫毒素。 在优选的实施方案中，本发明的毒素用于控制Phyllotreta属的跳蚤甲虫。 使用本文所述的基因，可以使用本领域技术人员已知的技术来实现植物的转化。 此外，本发明提供了优化用于在植物中表达的毒素基因。