Methods for Agrobacterium-mediated transformation
    4.
    发明授权
    Methods for Agrobacterium-mediated transformation 失效
    农杆菌介导转化方法

    公开(公告)号:US06822144B1

    公开(公告)日:2004-11-23

    申请号:US08963096

    申请日:1997-11-03

    IPC分类号: C12N1584

    CPC分类号: C12N15/8205

    摘要: The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.

    摘要翻译: 本发明涉及改善农杆菌介导的玉米胚转化的转化频率的方法。 使用农杆菌转化玉米的优选方法包括以下步骤:使来自玉米植物的至少一种未成熟胚与能够将至少一种基因转移至所述胚的农杆菌接触; 与土壤杆菌共培养胚胎; 在包含N6盐的培养基中培养胚胎,能够抑制农杆菌生长的抗生素,以及选择表达基因的胚胎的选择剂; 和再生表达该基因的植物。

    Agrobacterium mediated transformation of sorghum
    5.
    发明授权
    Agrobacterium mediated transformation of sorghum 失效
    农杆菌介导的高粱转化

    公开(公告)号:US06369298B1

    公开(公告)日:2002-04-09

    申请号:US09056418

    申请日:1998-04-07

    IPC分类号: A01H100

    CPC分类号: C12N15/8205

    摘要: Methods and compositions for the efficient transformation of sorghum is provided. The method involves infection with Agrobacterium, particularly those comprising a super-binary vector. In this manner, any gene of interest can be introduced into the sorghum plant. The transformed gene will be flanked by at least one T-DNA border and present in the transformed sorghum in low copy number. Transformed sorghum, cells, tissues, plants, and seed are also provided. The invention encompasses regenerated, fertile sorghum plants, transgenic seeds produced therefrom, T1 and subsequent generations.

    摘要翻译: 提供了高效转化高粱的方法和组成。 该方法涉及用农杆菌感染,特别是包含超二元载体的那些。 以这种方式,可以将任何感兴趣的基因引入高粱植物中。 转化的基因将侧接至少一个T-DNA边界并以低拷贝数存在于转化的高粱中。 还提供了转化的高粱,细胞,组织,植物和种子。 本发明包括再生的,可育的高粱植物,由其产生的转基因种子,T1和后代。

    Methods for agrobacterium-mediated transformation
    6.
    发明授权
    Methods for agrobacterium-mediated transformation 失效
    农杆菌介导转化方法

    公开(公告)号:US5981840A

    公开(公告)日:1999-11-09

    申请号:US788018

    申请日:1997-01-24

    CPC分类号: C12N15/8205

    摘要: The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.

    摘要翻译: 本发明涉及改善农杆菌介导的玉米胚转化的转化频率的方法。 使用农杆菌转化玉米的优选方法包括以下步骤:使来自玉米植物的至少一种未成熟胚与能够将至少一种基因转移至所述胚的农杆菌接触; 与土壤杆菌共培养胚胎; 在包含N6盐的培养基中培养胚胎,能够抑制农杆菌生长的抗生素,以及选择表达基因的胚胎的选择剂; 和再生表达该基因的植物。