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公开(公告)号：US20100235942A1
公开(公告)日：2010-09-16
申请号：US12729072
申请日：2010-03-22
申请人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
发明人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
CPC分类号： C12N15/8207 , C12N15/8205 , C12N15/8206 , C12N15/8209
摘要： The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.
摘要翻译： 本发明涉及一种用于产生转化玉米植物的新型转化系统。 特别地，本发明涉及一种在高温下的快速选择系统，其允许更快和更有效的转化。
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公开(公告)号：US08759609B2
公开(公告)日：2014-06-24
申请号：US12729072
申请日：2010-03-22
申请人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
发明人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
IPC分类号： C07K14/415 , A01H5/10 , A01H1/02
CPC分类号： C12N15/8207 , C12N15/8205 , C12N15/8206 , C12N15/8209
摘要： The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.
摘要翻译： 本发明涉及一种用于产生转化玉米植物的新型转化系统。 特别地，本发明涉及一种在高温下的快速选择系统，其允许更快和更有效的转化。
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公开(公告)号：US07682829B2
公开(公告)日：2010-03-23
申请号：US10709826
申请日：2004-06-01
申请人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
发明人： Tishu Cai , Shubha Subbarao , Yuechun Wan , Richard A. Yingling , Michael T. Mann , Anita Otal , David D. Songstad
CPC分类号： C12N15/8207 , C12N15/8205 , C12N15/8206 , C12N15/8209
摘要： The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.
摘要翻译： 本发明涉及一种用于产生转化玉米植物的新型转化系统。 特别地，本发明涉及一种在高温下的快速选择系统，其允许更快和更有效的转化。
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公开(公告)号：US08513016B2
公开(公告)日：2013-08-20
申请号：US13355312
申请日：2012-01-20
申请人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
发明人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
IPC分类号： C12N5/00
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要： A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
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公开(公告)号：US08124411B2
公开(公告)日：2012-02-28
申请号：US11848554
申请日：2007-08-31
申请人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
发明人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要： A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
摘要翻译： 证明了在单个容器中生产稳定转化的玉米植物的方法。 这种方法允许转换过程的自动化，并减少与传统植物组织培养系统相关的劳动力，材料和人体工学成本。
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公开(公告)号：US20080118981A1
公开(公告)日：2008-05-22
申请号：US11848554
申请日：2007-08-31
申请人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
发明人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要： A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
摘要翻译： 证明了在单个容器中生产稳定转化的玉米植物的方法。 这种方法允许转换过程的自动化，并减少与传统植物组织培养系统相关的劳动力，材料和人体工学成本。
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公开(公告)号：US20120180166A1
公开(公告)日：2012-07-12
申请号：US13355312
申请日：2012-01-20
申请人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
发明人： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要： A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
摘要翻译： 证明了在单个容器中生产稳定转化的玉米植物的方法。 这种方法允许转换过程的自动化，并减少与传统植物组织培养系统相关的劳动力，材料和人体工学成本。
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公开(公告)号：US07205459B1
公开(公告)日：2007-04-17
申请号：US09606808
申请日：2000-06-28
申请人： James R. Larkins , David D. Songstad , William L. Petersen , Hongyi Zhang , Michael T. Mann , T. Michael Spencer , Nancy G. Willetts
发明人： James R. Larkins , David D. Songstad , William L. Petersen , Hongyi Zhang , Michael T. Mann , T. Michael Spencer , Nancy G. Willetts
CPC分类号： A01H5/10 , A01H4/005 , C12N15/8205 , C12N15/8207
摘要： According to the invention, there is provided an inbred corn plant designated LIZL5. This invention thus relates to the plants, seeds and tissue cultures of the inbred corn plant LIZL5, and to methods for producing a corn plant produced by crossing the inbred corn plant LIZL5 with itself or with another corn plant, such as another inbred. This invention further relates to corn seeds and plants produced by crossing the inbred plant LIZL5 with another corn plant, such as another inbred, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of the inbred corn plant LIZL5, and also to the SSR and genetic isozyme typing profiles of inbred corn plant LIZL5. This invention further relates to methods of transforming the inbred LIZL5 and cells thereof, transformed plants produced by these methods, progeny transgenic plants, and seed derived therefrom.
摘要翻译： 根据本发明，提供了称为LIZL5的近交玉米植物。 因此，本发明涉及近交玉米植物LIZL5的植物，种子和组织培养物，以及通过将近交玉米植物LIZL5与其他玉米植物如另一近交系交叉产生的玉米植物的生产方法。 本发明还涉及通过将近交植物LIZL5与另一玉米植物（例如另一近交系）杂交并与相关物种杂交而产生的玉米种子和植物。 本发明还涉及近交玉米植物LIZL5的近交和杂交遗传互补，以及近交玉米植物LIZL5的SSR和遗传同工酶分型。 本发明还涉及转化近交LIZL5及其细胞，通过这些方法产生的转化植物，后代转基因植物和从其衍生的种子的方法。
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9.发明授权Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物公开(公告)号：US07705215B1
公开(公告)日：2010-04-27
申请号：US08113561
申请日：1993-08-25
申请人： Thomas R. Adams , Paul C. Anderson , Sheryl A. Chambers , Paul S. Chomet , Richard J. Daines , Christopher E. Flick , Kimberly Glassman , William J. Gordon-Kamm , Albert P. Kausch , Lucille B. Laccetti , Peggy G. Lemaux , Catherine J. Mackey , Mary Lou Mangano , Michael T. Mann , James V. O'Brien , Emil M. Orozco , Peter Orr , Thomas B. Rice , T. Michael Spencer , William G. Start , Michael A. Stephens , Clayton S. Vetsch , David A. Walters , Donald S. Walters , Nancy G. Willetts , Susan J. Zachwieja
发明人： Thomas R. Adams , Paul C. Anderson , Sheryl A. Chambers , Paul S. Chomet , Richard J. Daines , Christopher E. Flick , Kimberly Glassman , William J. Gordon-Kamm , Albert P. Kausch , Lucille B. Laccetti , Peggy G. Lemaux , Catherine J. Mackey , Mary Lou Mangano , Michael T. Mann , James V. O'Brien , Emil M. Orozco , Peter Orr , Thomas B. Rice , T. Michael Spencer , William G. Start , Michael A. Stephens , Clayton S. Vetsch , David A. Walters , Donald S. Walters , Nancy G. Willetts , Susan J. Zachwieja
CPC分类号： C12N15/8241 , A01H4/00 , A01H4/001 , C07K14/415 , C12N5/0025 , C12N5/04 , C12N9/0006 , C12N9/88 , C12N15/8207 , C12N15/8209 , C12N15/87
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。
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公开(公告)号：US20110271410A1
公开(公告)日：2011-11-03
申请号：US13104841
申请日：2011-05-10
申请人： Whitney Adams , Brandon Davis , Lubomyr Kucher , Brenda Lowe , Michael Spencer , Michael T. Mann
发明人： Whitney Adams , Brandon Davis , Lubomyr Kucher , Brenda Lowe , Michael Spencer , Michael T. Mann
CPC分类号： A01H4/003 , A01G7/00 , A01H4/001 , A01H4/008 , A01H5/10 , C12N15/82 , C12N15/8201 , C12N15/8205
摘要： The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.
摘要翻译： 本发明公开并要求用于快速机械分离适于转化或组织培养的单子叶植物组织的方法和装置。 本发明包括用于基本上分离靶植物组织以用作可转化外植体的机械装置，以及转基因植物和植物组织的繁殖。
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