公开(公告)号：US20190324033A1

公开(公告)日：2019-10-24

申请号：US15958931

申请日：2018-04-20

申请人： TOKITAE LLC ,  Cornell University

发明人： Luis F. Alonzo ,  Anne-Laure M. Le Ny ,  Kevin Paul Flood Nichols ,  Spencer Garing ,  Damian Madan ,  Sam Rasmussen Nugen ,  Troy Hinkley

IPC分类号： G01N33/569 ,  C12N15/73 ,  G01N33/543 ,  C12N7/02 ,  C12Q1/10 ,  C12Q1/66

摘要： Generally, this disclosure relates to expression constructs that encode a reporter enzyme-affinity binding tag fusion protein that is produced after the construct is inserted into bacteriophage and the bacteriophage infects bacteria. In some embodiments, the fusion protein is captured and produces a detectable signal. Signal intensity may correlate with the number of bacterial cells in a fluid sample. Methods of detecting bacteria using the expression constructs, and microfluidic devices for detecting bacteria using the expression constructs are also disclosed.

公开(公告)号：US20190217293A1

公开(公告)日：2019-07-18

申请号：US15870370

申请日：2018-01-12

申请人： Tokitae LLC

发明人： Luis F. Alonzo ,  Spencer Garing ,  Anne-Laure M. Le Ny ,  Kevin Paul Flood Nichols ,  Sam Rasmussen Nugen ,  John R. Williford

IPC分类号： B01L3/00 ,  B01D39/18 ,  G01N1/40 ,  C12Q1/04

CPC分类号： B01L3/502753 ,  B01D39/18 ,  B01D2239/1216 ,  B01L3/502738 ,  B01L2300/0681 ,  C12Q1/04 ,  G01N1/4077 ,  G01N2001/4088

摘要： A microfluidic device for concentrating and detecting bacteria in liquids, and related methods are described. The device includes a first filter chamber for capturing bacteria and performing incubations of the bacteria with one or more reagents, and a second filter chamber for capturing and concentrating a detectable material, with little or no binding of detectable material by the first filter. In an aspect, bacteria are incubated with growth media and engineered phage that cause the bacteria to produce an enzyme. In an aspect, the enzyme is capture in the second filter chamber and exposed to a substrate to produce a detectable signal.