公开(公告)号：US5294569A

公开(公告)日：1994-03-15

申请号：US9764

申请日：1993-01-27

申请人： Tomoh Masaki ,  Masashi Yanagisawa ,  Akihiro Inoue

发明人： Tomoh Masaki ,  Masashi Yanagisawa ,  Akihiro Inoue

IPC分类号： A61K38/00 ,  C07K14/575 ,  C12N1/21 ,  C12N15/12 ,  C12N15/16 ,  C07H15/12 ,  C12N1/22 ,  C12P21/06

CPC分类号： C07K14/57536 ,  A61K38/00 ,  Y10S930/26

摘要： Disclosed are (1) a DNA sequence containing a segment coding for endothelin-2 or human endothelin-3, (2) a precursor protein and a mature peptide of endothelin-2 or human endothelin-3, (3) a transformant carrying a DNA sequence containing a DNA segment coding for endothelin-2 or human endothelin-3 and (4) a method for preparing mature endothelin-2 or endothelin-3 which comprises culturing the transformant described in (3), producing and accumulating a protein in a culture medium, and collecting the same.Cells transfected or transformed with the DNA allow large amounts of endothelin-2 or endothelin-3 to be produced, which causes the advantageous production of endothelin-2 or endothelin-3.Endothelin-2 and endothelin-3 can be utilized as hypotension therapeutic agents or local vasoconstrictors to animals including humans.

摘要翻译： 公开了（1）含有编码内皮素-2或人内皮素-3的区段的DNA序列，（2）内皮素-2或人内皮素-3的前体蛋白质和成熟肽，（3）携带DNA的转化体 包含编码内皮素-2或人内皮素-3的DNA区段的序列和（4）制备成熟内皮素-2或内皮素-3的方法，其包括培养（3）中所述的转化体，在培养物中产生和积累蛋白质 中等，收集相同。 用DNA转染或转化的细胞允许产生大量的内皮素-2或内皮素-3，这导致有利地产生内皮素-2或内皮素-3。 内皮素-2和内皮素-3可用作包括人在内的动物的低血压治疗剂或局部血管收缩剂。

公开(公告)号：US5231166A

公开(公告)日：1993-07-27

申请号：US422132

申请日：1989-10-16

申请人： Tomoh Masaki ,  Masashi Yanagisawa ,  Akihiro Inoue

发明人： Tomoh Masaki ,  Masashi Yanagisawa ,  Akihiro Inoue

IPC分类号： A61K38/00 ,  C07K14/575 ,  C12N1/21 ,  C12N15/12 ,  C12N15/16

CPC分类号： C07K14/57536 ,  A61K38/00 ,  Y10S930/26

摘要： Endothelin-2 and a big type of endothelin-2 proteins are described. The proteins contain the amino acid sequence: Cys Ser Cys Ser Ser Trp Leu Asp Lys Glu Cys Val Tyr Phe Cys His Leu Asp Ile Ile Trp.

公开(公告)号：US5811263A

公开(公告)日：1998-09-22

申请号：US645771

申请日：1996-05-14

申请人： Tomoh Masaki ,  Masashi Yanagisawa ,  Akihiro Inoue

发明人： Tomoh Masaki ,  Masashi Yanagisawa ,  Akihiro Inoue

IPC分类号： A61K38/00 ,  C07K14/575 ,  C12N1/21 ,  C12N15/12 ,  C12N15/16 ,  C12N5/10 ,  C12N15/63

CPC分类号： C07K14/57536 ,  A61K38/00 ,  Y10S930/26

摘要： A cloned DNA molecule is disclosed. The DNA molecule encodes a human endothelin-3 protein having the amino acid sequence: Glu-Gly-Ala-Pro-Glu-His-His-Arg-Ser-Arg-Arg-Cys-Thr-Cys-Phe-Thr-Tyr-Lys-Asp-Lys-Glu-Cys-Val-Tyr-Tyr-Cys-His-Leu-Asp-Ile-Ile-Trp-Ile-Asn-Thr-Pro-Glu or any portion of said amino acid sequence containing Cys-Thr-Cys-Phe-Thr-Tyr-Lys-Asp-Lys-Glu-Cys-Val-Tyr-Tyr-Cys-His-Leu-Asp-Ile-Ile-Trp. Also disclosed is a method of producing mature endothelin-3 protein. In the method, the host cell is cultured, allowing mature endothelin-3 to accumulate in the culture medium, and the mature endothelin-3 protein is separated from the culture medium.

摘要翻译： 公开了克隆的DNA分子。 DNA分子编码具有以下氨基酸序列的人内皮素-3蛋白：Glu-Gly-Ala-Pro-Glu-His-His-Arg-Ser-Arg-Arg-Cys-Thr-Cys-Phe-Thr-Tyr- Lys-Asp-Lys-Glu-Cys-Val-Tyr-Tyr-Cys-His-Leu-Asp-Ile-Ile-Trp-Ile-Asn-Thr-Pro-Glu或含有Cys- Thr-Cys-Phe-Thr-Tyr-Lys-Asp-Lys-Glu-Cys-Val-Tyr-Tyr-Cys-His-Leu-Asp-Ile-Ile-Trp。 还公开了生产成熟内皮素-3蛋白的方法。 在该方法中，培养宿主细胞，允许成熟的内皮素-3在培养基中积累，并将成熟的内皮素-3蛋白从培养基中分离。

公开(公告)号：US5548061A

公开(公告)日：1996-08-20

申请号：US163030

申请日：1993-12-06

申请人： Tomoh Masaki ,  Masashi Yanagisawa ,  Akihiro Inoue

发明人： Tomoh Masaki ,  Masashi Yanagisawa ,  Akihiro Inoue

IPC分类号： A61K38/00 ,  C07K14/575 ,  C12N1/21 ,  C12N15/12 ,  C12N15/16 ,  C07K14/00 ,  A61K38/16

CPC分类号： C07K14/57536 ,  A61K38/00 ,  Y10S930/26

摘要： Disclosed are (1) a DNA sequence containing a DNA segment coding for endothelin-2 or human endothelin-3, (2) a precursor protein and a mature peptide of endothelin-2 or human endothelin-3, (3) a transformant carrying a DNA sequence containing a DNA segment coding for endothelin-2 or human endothelin-3 and (4) a method for preparing mature endothelin-2 or endothelin-3 which comprises culturing the transformant described in (3), producing and accumulating a protein in a culture medium, and collecting the same. Cells transfected or transformed with the DNA allow large amounts of endothelin-2 or endothelin-3 to be produced, which causes the advantageous production of endothelin-2 or endothelin-3. Endothelin-2 and endothelin-3 can be utilized as hypotension therapeutic agents or local vasoconstrictors to animals including humans.

摘要翻译： 公开了（1）含有编码内皮素-2或人内皮素-3的DNA区段的DNA序列，（2）前体蛋白质和内皮素-2或人内皮素-3的成熟肽，（3）携带 含有编码内皮素-2或人内皮素-3的DNA区段的DNA序列和（4）制备成熟内皮素-2或内皮素-3的方法，其包括培养（3）中所述的转化体，产生和积累蛋白质 培养基，并收集。 用DNA转染或转化的细胞允许产生大量的内皮素-2或内皮素-3，这导致有利地产生内皮素-2或内皮素-3。 内皮素-2和内皮素-3可用作包括人在内的动物的低血压治疗剂或局部血管收缩剂。

公开(公告)号：US4981950A

公开(公告)日：1991-01-01

申请号：US249429

申请日：1988-09-26

申请人： Tomoh Masaki ,  Katsutoshi Goto ,  Sadao Kimura ,  Youji Mitsui ,  Yoshio Yazaki ,  Masashi Yanagisawa ,  Hiroki Kurihara

发明人： Tomoh Masaki ,  Katsutoshi Goto ,  Sadao Kimura ,  Youji Mitsui ,  Yoshio Yazaki ,  Masashi Yanagisawa ,  Hiroki Kurihara

IPC分类号： A61K38/00 ,  C07K14/575

CPC分类号： C07K14/57536 ,  A61K38/00

摘要： Disclosed is a vasoconstrictor peptide called Endothelin having a molecular weight of 2,500.+-.300. Endothelin contains 21 amino acid residues, wherein four cysteines form two sets of S--S bond.In order to obtain the vasoconstrictor factors of the present invention, it is preferable to culture the endothelial cells collected from the vascular inner walls in a serum-free medium.The vasoconstrictor peptides of the present invention can be utilized as hypotension therapeutic agents or local vasoconstrictors to animals including humans.

公开(公告)号：US5844133A

公开(公告)日：1998-12-01

申请号：US915223

申请日：1997-08-20

申请人： Kazuya Goto ,  Masashi Yanagisawa ,  Toshihiko Gomi

发明人： Kazuya Goto ,  Masashi Yanagisawa ,  Toshihiko Gomi

IPC分类号： E21B19/08 ,  E21B19/084 ,  E21B44/00

CPC分类号： E21B44/00

摘要： A drilling control apparatus for automatically controlling the drilling operation of a rock drill includes a control unit for storing known criteria drilling patterns for various rock properties. A specific drilling pattern is selected based upon a collaring procedure for operation of the rock drill. A detector continually monitors variables such as rotational pressure, feed pressure and flushing pressure during the drilling process to determine a change in condition of the rock for determination of automatic selection of another more appropriate drilling pattern.

摘要翻译： 用于自动控制凿岩机钻孔操作的钻孔控制装置包括用于存储用于各种岩石特性的已知标准钻孔图案的控制单元。 基于钻凿操作的碰撞过程来选择特定钻孔图案。 检测器在钻井过程中连续监测诸如旋转压力，进料压力和冲洗压力之类的变量，以确定岩石状态的变化，以确定另一种更合适的钻孔模式的自动选择。

公开(公告)号：US08258163B2

公开(公告)日：2012-09-04

申请号：US12478753

申请日：2009-06-04

申请人： Masashi Yanagisawa

发明人： Masashi Yanagisawa

IPC分类号： A61K31/4168

CPC分类号： A61K31/4168 ,  A61K31/496 ,  A61K31/506 ,  A61K31/5377

摘要： Methods and compositions for agonizing a type-2 orexin receptor (OX2R) in a cell determined to be in need thereof, including the general method of (a) administering to a subject a cyclic guanidinyl OX2R agonist and (b) detecting a resultant enhanced wakefulness or increased resistance to diet-induced accumulation of body fat, or abbreviated recovery from general anesthesia or jet lag.

摘要翻译： 包括通常的方法（a）向受试者施用环状胍基OX2R激动剂和（b）检测所得到的增强的觉醒性的方法和组合物，所述方法和组合物用于激活确定为需要的细胞中的2型食欲蛋白受体（OX2R） 或增加对饮食诱导的身体脂肪积累的抵抗力，或缩小从全身麻醉或射精滞后的恢复。

公开(公告)号：US06309854B1

公开(公告)日：2001-10-30

申请号：US08939093

申请日：1997-09-26

申请人： Derk J. Bergsma ,  David P. Brooks ,  Miklos Gellai ,  Shelagh Wilson ,  Masashi Yanagisawa

发明人： Derk J. Bergsma ,  David P. Brooks ,  Miklos Gellai ,  Shelagh Wilson ,  Masashi Yanagisawa

IPC分类号： C07H2104

CPC分类号： C07K14/575 ,  A61K38/00 ,  C07K16/26

摘要： Polypeptides of HFGAN72 receptor ligands and polynucleotides encoding the polypeptides are provided. Methods of using these polypeptides to diagnose diseases relating to the under- or over-expression of HFGAN72 receptor ligands are also provided. In addition, methods of identifying agonists or antagonists of the interaction of HFGAN72 receptor ligands with the HFGAN72 receptor are provided. Methods of treatment by administering the identified agonists or antagonists to patients in need thereof are further disclosed.

摘要翻译： 提供HFGAN72受体配体的多肽和编码多肽的多核苷酸。 还提供了使用这些多肽诊断与HFGAN72受体配体的低表达或过度表达有关的疾病的方法。 另外，提供了识别HFGAN72受体配体与HFGAN72受体相互作用的激动剂或拮抗剂的方法。 还公开了通过将所鉴定的激动剂或拮抗剂给予需要的患者的治疗方法。

公开(公告)号：US06200360B1

公开(公告)日：2001-03-13

申请号：US09289954

申请日：1999-04-13

申请人： Tomoyasu Imai ,  Hiroaki Asano ,  Hayashi Kodama ,  Masato Kitajima ,  Masashi Yanagisawa

发明人： Tomoyasu Imai ,  Hiroaki Asano ,  Hayashi Kodama ,  Masato Kitajima ,  Masashi Yanagisawa

IPC分类号： B24D300

CPC分类号： B24D18/0009 ,  B24D3/06 ,  B24D3/10 ,  B24D18/0018 ,  Y10T428/24372

摘要： An abrasive tool includes an electroformed layer having superabrasive grains electroplated on an outer surface of the electroformed layer, and a plurality of dimples arranged on the outer surface of the electroformed layer using a mold with projections made of gel adhesive. The concentration of the abrasive gains is regulated by changing the number of the dimples (i.e., changing a dimple-area-rate). The gel adhesive preferably has a viscosity of 500,000 cP or smaller. The dimple-area-rate is preferably from 7 to 70%.

摘要翻译： 研磨工具包括电铸层，其电镀在电铸层的外表面上，并且使用具有由凝胶粘合剂制成的凸起的模具布置在电铸层的外表面上的多个凹坑。 通过改变凹坑的数量（即，改变凹坑面积率）来调节磨料增益的浓度。 凝胶粘合剂优选具有500,000cP以下的粘度。 凹坑面积率优选为7至70％。

公开(公告)号：US5736376A

公开(公告)日：1998-04-07

申请号：US574763

申请日：1995-12-19

申请人： Masashi Yanagisawa

发明人： Masashi Yanagisawa

IPC分类号： A61K39/00 ,  C12N9/64 ,  C12N9/48 ,  C07H21/04 ,  C12P19/34 ,  C12P21/06

CPC分类号： C12N9/6497 ,  C12Y304/24071 ,  A61K39/00 ,  Y10S435/814

摘要： Disclosed is a nucleic acid segment encoding endothelin converting enzyme-2 (ECE-2), that produces mature ET-1 from big ET-1 both in vitro and in transfected cells. Also disclosed is a polypeptide composition comprising partially purified ECE-2 and methods of using ECE-2 to screen candidate substances as inhibitors of ECE-2. Methods of producing recombinant ECE-2 are also disclosed.

摘要翻译： 公开了一种编码内皮素转化酶-2（ECE-2）的核酸片段，其在体外和转染的细胞中都能从大的ET-1产生成熟的ET-1。 还公开了包含部分纯化的ECE-2的多肽组合物和使用ECE-2筛选候选物质作为ECE-2抑制剂的方法。 还公开了重组ECE-2的制备方法。